L-a-Hydroxyglutaric acid disodium salt
(Synonyms: S-2-羟基戊二酸,(S)-2-Hydroxyglutaric acid disodium) 目录号 : GC11149
An α-hydroxy acid
Cas No.:63512-50-5
Sample solution is provided at 25 µL, 10mM.
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Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
IC50: N/A
L-α-Hydroxyglutaric acid disodium salt is a salt form of L-α-Hydroxyglutaric acid. L-α-Hydroxyglutaric acid accumulates as a result of a rare defect in L-2-HG dehydogenase, resulting in the metabolic disorder L-α-hydroxyglutaric aciduria.
In vitro: It has been reported that L-α-Hydroxyglutaric acid disodium salt could be used as the collection buffer for increasing the recovery of hypoxia-inducible factor-1 α, which was a marker of hypoxia in human tumors [1]. In another previous study, L-α-Hydroxyglutaric acid disodium salt was also used in the radiolabeled conversion assay to evaluate its effect on the TET family of methyl hydroxylases [2].
In vivo: In the 30-day-old rats, L-α-Hydroxyglutaric acid was found to be able to significantly increase chemiluminescence, PCF and TBA-RS measurements and decrease the TAR values in cerebellum markedly. In addition, the L-α-Hydroxyglutaric acid-induced increase of TBA-RS was significantly attenuated by melatonin and also by the combinations of ascorbic acid and soluble alpha-tocopherol (Trolox) and of superoxide dismutase plus catalase but not by the inhibitor of nitric oxide synthase Nomega-nitro-L-arginine methyl ester (L-NAME), , superoxide dismutase or creatine or catalase alone in either cerebral structure [3].
Clinical trial: Up to now, L-a-Hydroxyglutaric acid disodium salt is still in the preclinical development stage.
References:
[1] Park SR,Kinders RJ,Khin S,et al. Validation of a hypoxia-inducible factor-1 alpha specimen collection procedure and quantitative enzyme-linked immunosorbent assay in solid tumor tissues. Anal Biochem.2014 Aug 15;459:1-11.
[2] Evans B,Griner E. Registered report: Oncometabolite 2-hydroxyglutarate is a competitive inhibitor of α-ketoglutarate-dependent dioxygenases. Elife.2015 Jul 31;4:e07420.
[3] Latini A,Scussiato K,Rosa RB,Leipnitz G,Llesuy S,Belló-Klein A,Dutra-Filho CS,Wajner M. Induction of oxidative stress by L-2-hydroxyglutaric acid in rat brain. J Neurosci Res.2003 Oct 1;74(1):103-10.
| Cas No. | 63512-50-5 | SDF | |
| 别名 | S-2-羟基戊二酸,(S)-2-Hydroxyglutaric acid disodium | ||
| 化学名 | sodium (S)-2-hydroxypentanedioate | ||
| Canonical SMILES | O=C([O-])[C@@H](O)CCC([O-])=O.[Na+].[Na+] | ||
| 分子式 | C5H6Na2O5 | 分子量 | 192.08 |
| 溶解度 | ≥ 19.2mg/mL in Water | 储存条件 | Store at -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 5.2062 mL | 26.0308 mL | 52.0616 mL |
| 5 mM | 1.0412 mL | 5.2062 mL | 10.4123 mL |
| 10 mM | 0.5206 mL | 2.6031 mL | 5.2062 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。