L-Alloisoleucine ((3R)-LS-Isoleucine)
(Synonyms: L-别异亮氨酸; (3R)-LS-Isoleucine; L-Allo-isoleucine) 目录号 : GC30624
A branched-chain amino acid
Cas No.:1509-34-8
Sample solution is provided at 25 µL, 10mM.
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Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
L-Alloisoleucine is a branched-chain amino acid and diastereomer of L-isoleucine.1 It is formed from L-isoleucine by transamination.2 Plasma L-alloisoleucine levels are increased in patients with maple syrup urine disease (MSUD), an inborn error of metabolism characterized by a deficiency in the branched-chain α-ketoacid dehydrogenase complex, the complex that catalyzes the degradation of branched-chain amino acids, leading to the accumulation of branched-chain amino acids in the plasma and urine, a maple syrup-like odor in the urine, and neurological impairments.1
1.Schadewaldt, P., Bodner-Leidecker, A., Hammen, H.W., et al.Significance of ?-alloisoleucine in plasma for diagnosis of maple syrup urine diseaseClin. Chem.45(10)1734-1740(1999) 2.Schadewaldt, P., Bodner-Leidecker, A., Hammen, H.W., et al.Formation of L-alloisoleucine in vivo: An L-[13C]isoleucine study in manPediatr. Res.47(2)271-277(2000)
| 制备储备液 | |||
 |
1 mg | 5 mg | 10 mg |
| 1 mM | 7.6237 mL | 38.1185 mL | 76.2369 mL |
| 5 mM | 1.5247 mL | 7.6237 mL | 15.2474 mL |
| 10 mM | 0.7624 mL | 3.8118 mL | 7.6237 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。