L-Ascorbic Acid 2-phosphate (magnesium salt)
(Synonyms: 维生素C磷酸酯镁; 2-Phospho-L-ascorbic acid magnesium) 目录号 : GC19511
L-Ascorbic Acid 2-phosphate (magnesium salt) (AA2P)是一种稳定的VC(维生素C)衍生物。
Cas No.:113170-55-1
Sample solution is provided at 25 µL, 10mM.
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- Purity: >99.00%
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- Datasheet
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Cell experiment [1]: |
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Cell lines |
SH-SY5Y cells |
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Preparation method |
SH-SY5Y cells were first prepared with 100-500 μM L-Ascorbic Acid 2-phosphate in 1×PBS and treated with 1 μM MeHg for 24 h. |
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Reaction Conditions |
100-500μM; 24 h |
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Applications |
L-Ascorbic Acid 2-phosphate (magnesium salt) decreases MeHg-induced ROS accumulation in cells in a dose-dependent manner. |
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Animal experiment [2]: |
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Animal models |
C57BL/6 mice |
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Preparation method |
Mice back skins were shaved at 7 weeks of age (telogen stage) and then vehicle or L-Ascorbic Acid 2-phosphate at 250 mM were applied by iontophoresis. The unique iontophoresis system was adapted, which provides a constant electric current of 0.1-1.0 mA/cm2 areas. Lontophoresis was performed for 15 min at 0.2-0.4 mV constant currents daily for 4 weeks. |
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Applications |
L-Ascorbic Acid 2-phosphate (magnesium salt) regulate mesenchymal–epithelial interactions in the hair follicle and promote hair growth in mice models. |
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References: [1]. Zuo K, Xu Q, et,al. L-Ascorbic Acid 2-Phosphate Attenuates Methylmercury-Induced Apoptosis by Inhibiting Reactive Oxygen Species Accumulation and DNA Damage in Human SH-SY5Y Cells. Toxics. 2023 Feb 2;11(2):144. doi: 10.3390/toxics11020144. PMID: 36851019; PMCID: PMC9967424. [2]. Sung YK, Hwang SY, et,al. The hair growth promoting effect of ascorbic acid 2-phosphate, a long-acting Vitamin C derivative. J Dermatol Sci. 2006 Feb;41(2):150-2. doi: 10.1016/j.jdermsci.2005.11.010. Epub 2006 Jan 9. PMID: 16406749. |
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L-Ascorbic Acid 2-phosphate (magnesium salt) (AA2P) is a stable VC(vitamin C) derivative[1]. It can stimulate the expression and formation of collagen[2].
L-Ascorbic Acid 2-phosphate (magnesium salt) (100-500μM; 24 h) attenuates MeHg-induced apoptosis by alleviating ROS-mediated DNA damage in SH-SY5Y cell [3]. L-Ascorbic Acid 2-phosphate (magnesium salt) (0.25-1mM) repressed dihydrotestosterone (DHT)-induced DHT-inducible dickkopf-1 (DKK-1) protein expression[4]. L-Ascorbic Acid 2-phosphate (magnesium salt) (250μM; 5-60min) co-treatment with FGF-2 induced optimal proliferation of Human bone marrow mesenchymal stem cells (BMSC) and increased the accumulation rate of BMSC numbers during a 2-month culture period[5]. IGF-1 mRNA in Dermal papilla (DP) cells was upregulated and IGF-1 protein in the conditioned medium of DP cells was significantly increased after treatment with L-Ascorbic Acid 2-phosphate (magnesium salt), L-Ascorbic Acid 2-phosphate (magnesium salt)-inducible IGF-1 from DP cells promotes proliferation of follicular keratinocytes and stimulates hair follicle growth in vitro via PI3K[6].
L-Ascorbic Acid 2-phosphate (magnesium salt) regulate mesenchymal–epithelial interactions in the hair follicle and promote hair growth in mice models[7].
References:
[1]. Spiclin P, Homar M, et,al. Sodium ascorbyl phosphate in topical microemulsions. Int J Pharm. 2003 Apr 30;256(1-2):65-73. doi: 10.1016/s0378-5173(03)00063-2. PMID: 12695012.
[2]. Shima N, Kimoto M, et,al. Increased proliferation and replicative lifespan of isolated human corneal endothelial cells with L-ascorbic acid 2-phosphate. Invest Ophthalmol Vis Sci. 2011 Nov 7;52(12):8711-7. doi: 10.1167/iovs.11-7592. PMID: 21980003.
[3]. Zuo K, Xu Q, et,al. L-Ascorbic Acid 2-Phosphate Attenuates Methylmercury-Induced Apoptosis by Inhibiting Reactive Oxygen Species Accumulation and DNA Damage in Human SH-SY5Y Cells. Toxics. 2023 Feb 2;11(2):144. doi: 10.3390/toxics11020144. PMID: 36851019; PMCID: PMC9967424.
[4]. Kwack MH, Kim MK, et,al. L-ascorbic acid 2-phosphate represses the dihydrotestosterone-induced dickkopf-1 expression in human balding dermal papilla cells. Exp Dermatol. 2010 Dec;19(12):1110-2. doi: 10.1111/j.1600-0625.2010.01143.x. PMID: 20701628.
[5]. Bae SH, Ryu H, et,al. L-ascorbic acid 2-phosphate and fibroblast growth factor-2 treatment maintains differentiation potential in bone marrow-derived mesenchymal stem cells through expression of hepatocyte growth factor. Growth Factors. 2015 Apr;33(2):71-8. doi: 10.3109/08977194.2015.1013628. Epub 2015 Feb 25. PMID: 25714612.
[6]. Kwack MH, Shin SH, et,al. l-Ascorbic acid 2-phosphate promotes elongation of hair shafts via the secretion of insulin-like growth factor-1 from dermal papilla cells through phosphatidylinositol 3-kinase. Br J Dermatol. 2009 Jun;160(6):1157-62. doi: 10.1111/j.1365-2133.2009.09108.x. Epub 2009 Mar 26. PMID: 19416266.
[7]. Sung YK, Hwang SY, et,al. The hair growth promoting effect of ascorbic acid 2-phosphate, a long-acting Vitamin C derivative. J Dermatol Sci. 2006 Feb;41(2):150-2. doi: 10.1016/j.jdermsci.2005.11.010. Epub 2006 Jan 9. PMID: 16406749.
L-Ascorbic Acid 2-phosphate (magnesium salt) (AA2P)是一种稳定的VC(维生素C)衍生物[1]。它可以刺激胶原蛋白的表达和形成[2]。
L-Ascorbic Acid 2-phosphate (magnesium salt) (100-500μM; 24 h)通过减轻ROS介导的SH-SY5Y细胞DNA损伤来减轻MeHg诱导的细胞凋亡[3]。L-Ascorbic Acid 2-phosphate (magnesium salt) (0.25-1mM)抑制dihydrotestosterone (DHT)诱导的DHT-inducible dickkopf-1 (DKK-1)蛋白表达[4]。L-Ascorbic Acid 2-phosphate (magnesium salt) (250μM; 5-60min)与FGF-2共处理诱导骨髓间充质干细胞的最佳增殖，并在2个月的培养期间增加了骨髓间充质干细胞数量的积累速率[5]。L-Ascorbic Acid 2-phosphate (magnesium salt)处理后，Dermal papilla (DP)细胞中IGF-1 mRNA表达上调，DP细胞条件培养基中IGF-1蛋白水平显著升高，L-Ascorbic Acid 2-phosphate (magnesium salt)诱导的DP细胞中IGF-1通过PI3K促进毛囊角质形成细胞增殖，刺激毛囊生长[6]。
L-Ascorbic Acid 2-phosphate (magnesium salt)促进培养的人毛囊毛轴的伸长，并诱导小鼠毛发生长[7]。
| Cas No. | 113170-55-1 | SDF | |
| 别名 | 维生素C磷酸酯镁; 2-Phospho-L-ascorbic acid magnesium | ||
| 化学名 | L-Ascorbic acid is essential for the synthesis of collagen | ||
| Canonical SMILES | O=C1C(OP([O-])([O-])=O)=C([C@]([H])(O1)[C@H](CO)O)[O-].O=C2C(OP([O-])([O-])=O)=C([C@]([H])(O2)[C@H](CO)O)[O-].[Mg+2].[Mg+2].[Mg+2] GC47538: O=C1C(OP([O-])([O-])=O)=C([C@]([H])(O1)[C@H](CO)O)[O-].O=C2C(OP([O-])([O-])=O)=C([C@]([H])(O2)[C@H](CO)O)[O-].[Mg+2].[Mg+2].[Mg+2] | ||
| 分子式 | C6H6O9P • 1.5Mg | 分子量 | 289.5 |
| 溶解度 | PBS (pH 7.2): 1 mg/ml | 储存条件 | Store at -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 3.4542 mL | 17.2712 mL | 34.5423 mL |
| 5 mM | 0.6908 mL | 3.4542 mL | 6.9085 mL |
| 10 mM | 0.3454 mL | 1.7271 mL | 3.4542 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
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