L-Isoleucine-13C6
(Synonyms: L-(+)-Isoleucine-13C6) 目录号 : GC52323
An internal standard for the quantification of L-isoleucine
Cas No.:201740-82-1
Sample solution is provided at 25 µL, 10mM.
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Quality Control & SDS
- View current batch:
- Purity: >95.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
L-Isoleucine-13C6 is intended for use as an internal standard for the quantification of L-isoleucine by GC- or LC-MS. L-Isoleucine is an essential and branched-chain amino acid.1 It is an isomer of L-leucine and a diastereomer of L-alloisoleucine that functions as a nitrogen donor and has roles in glucose consumption, fatty acid metabolism, and immune function.1,2 Urinary levels of L-isoleucine are increased in patients with deficiencies in β-ketothiolase, short/branched-chain specific acyl-CoA dehydrogenase (SBCAD), or 2-methyl-3-hydroxybutyryl-CoA dehydrogenase (MHBD), inborn errors of metabolism characterized by episodic ketoacidosis and neurological impairments.3
1.Nie, C., He, T., Zhang, W., et al.Branched chain amino acids: Beyond nutrition metabolismInt. J. Mol. Sci.19(4)954(2018) 2.Zhang, S., Zeng, X., Ren, M., et al.Novel metabolic and physiological functions of branched chain amino acids: A reviewJ. Anim. Sci. Biotechnol.810(2017) 3.Korman, S.H.Inborn errors of isoleucine degradation: A reviewMol. Genet. Metab.89(4)289-299(2006)
| 制备储备液 | |||
 |
1 mg | 5 mg | 10 mg |
| 1 mM | 7.2939 mL | 36.4697 mL | 72.9395 mL |
| 5 mM | 1.4588 mL | 7.2939 mL | 14.5879 mL |
| 10 mM | 0.7294 mL | 3.647 mL | 7.2939 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。