L-Leucine-d3
(Synonyms: L-亮氨酸-D3) 目录号 : GC64188
L-Leucine-d3是L-Leucine的氘代物，用于蛋白质的质谱（MS）定量测定和作为代谢研究中的示踪剂。
Cas No.:87828-86-2
Sample solution is provided at 25 µL, 10mM.
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L-Leucine-d3 is a deuterated form of L-Leucine that is used for mass spectrometry (MS) quantification of proteins and as a tracer in metabolic studies[1, 2]. L-Leucine is an essential branched-chain amino acid (BCAA) that activates the mammalian target of rapamycin (mTOR) signaling pathway[3]. L-Leucine is an activator of glutamine:6-phosphofructotransferase (GFAT) and can inhibit NO synthesis in endothelial cells[4]. L-Leucine can be used by skeletal muscle to provide energy during exercise[5]. Both L-Leucine and D-Leucine can effectively inhibit epileptic seizures in mice[6].
References:
[1] Pannkuk E L, Laiakis E C, Girgis M, et al. Temporal effects on radiation responses in nonhuman primates: identification of biofluid small molecule signatures by gas chromatography–mass spectrometry metabolomics[J]. Metabolites, 2019, 9(5): 98.
[2] Zhou J, Liang S, Fang L, et al. Quantitative proteomic analysis of HepG2 cells treated with quercetin suggests IQGAP1 involved in quercetin-induced regulation of cell proliferation and migration[J]. OMICS A Journal of Integrative Biology, 2009, 13(2): 93-103.
[3] Duan Y, Li F, Liu H, et al. Nutritional and regulatory roles of leucine in muscle growth and fat reduction[J]. Front Biosci (Landmark Ed), 2015, 20(4): 796-813.
[4] Yang Y, Wu Z, Meininger C J, et al. L-Leucine and NO-mediated cardiovascular function[J]. Amino Acids, 2015, 47: 435-447.
[5] Mero A. Leucine supplementation and intensive training[J]. Sports Medicine, 1999, 27: 347-358.
[6] Hartman A L, Santos P, O'Riordan K J, et al. Potent anti-seizure effects of D-leucine[J]. Neurobiology of Disease, 2015, 82: 46-53.
| Cas No. | 87828-86-2 | SDF | Download SDF |
| 别名 | L-亮氨酸-D3 | ||
| 分子式 | C6H10D3NO2 | 分子量 | 134.19 |
| 溶解度 | H2O : ≥ 10 mg/mL (74.52 mM) | 储存条件 | Store at -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
 |
1 mg | 5 mg | 10 mg |
| 1 mM | 7.4521 mL | 37.2606 mL | 74.5212 mL |
| 5 mM | 1.4904 mL | 7.4521 mL | 14.9042 mL |
| 10 mM | 0.7452 mL | 3.7261 mL | 7.4521 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet