L-Ornithine ((S)-2,5-Diaminopentanoic acid)
(Synonyms: 鸟氨酸) 目录号 : GC30044
L-ornithine ((S)-2,5-Diaminopentanoic acid)是一种天然存在的非蛋白质氨基酸，在人体的尿素循环中发挥重要作用。
Cas No.:70-26-8
Sample solution is provided at 25 µL, 10mM.
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L-ornithine ((S)-2,5-Diaminopentanoic acid) is a naturally occurring non-protein amino acid that plays an important role in the body's urea cycle. L-ornithine is not directly involved in the construction of proteins and is mainly used in the urea cycle to remove excess nitrogen from the body. L-ornithine has renal protection [1-3].
L-ornithine(300μM; 24h) activates Ca2+ signaling to exert its protective function on human proximal tubular cells[4]. L-ornithine (1–40mM) decreased cell impedance and elevated the BBB model permeability in primary rat brain endothelial cells[5].
L-ornithine(0.1875, 0.75 or 3mmol/10ml/kg; p.o) administered orally elevates brain L-ornithine levels and has an anxiolytic-like effect in mice[6]. Systemic L-ornithine(500mg/kg; p.o) supplementation specifically increases ovarian putrescine levels during ovulation in mice[7].
References:
[1]. Demura S, Morishita K, et,al. Effect of L-ornithine hydrochloride ingestion on intermittent maximal anaerobic cycle ergometer performance and fatigue recovery after exercise. Eur J Appl Physiol. 2011 Nov;111(11):2837-43. doi: 10.1007/s00421-011-1896-1. Epub 2011 Mar 23. PMID: 21431425.
[2]. Wu G, Morris SM Jr. Arginine metabolism: nitric oxide and beyond. Biochem J. 1998 Nov 15;336 ( Pt 1)(Pt 1):1-17. doi: 10.1042/bj3360001. PMID: 9806879; PMCID: PMC1219836.
[3]. Morris SM Jr. Regulation of enzymes of the urea cycle and arginine metabolism. Annu Rev Nutr. 2002;22:87-105. doi: 10.1146/annurev.nutr.22.110801.140547. Epub 2002 Jan 4. PMID: 12055339.
[4]. Shin S, Gombedza FC, Bandyopadhyay BC. l-ornithine activates Ca2+ signaling to exert its protective function on human proximal tubular cells. Cell Signal. 2020 Mar;67:109484. doi: 10.1016/j.cellsig.2019.109484. Epub 2019 Nov 23. PMID: 31770578; PMCID: PMC7302702.
[5].Walter FR, Harazin A, et,al. Blood-brain barrier dysfunction in L-ornithine induced acute pancreatitis in rats and the direct effect of L-ornithine on cultured brain endothelial cells. Fluids Barriers CNS. 2022 Feb 17;19(1):16. doi: 10.1186/s12987-022-00308-0. PMID: 35177109; PMCID: PMC8851707.
[6]. Kurata K, Nagasawa M, et,al. Orally administered L-ornithine elevates brain L-ornithine levels and has an anxiolytic-like effect in mice. Nutr Neurosci. 2011 Nov;14(6):243-8. doi: 10.1179/1476830511Y.0000000018. PMID: 22053755.
[7]. Lavergne CLJ, Tao Y,et,al. Systemic L-ornithine supplementation specifically increases ovarian putrescine levels during ovulation in mice†. Biol Reprod. 2022 Apr 26;106(4):792-801. doi: 10.1093/biolre/ioab233. PMID: 34935905.
L-ornithine ((S)-2,5-Diaminopentanoic acid)是一种天然存在的非蛋白质氨基酸，在人体的尿素循环中发挥重要作用。L-ornithine不直接参与蛋白质的合成，主要用于尿素循环中去除体内多余的氮。L-ornithine具有肾脏保护作用[1-3]。
L-ornithine(300μM; 24h)激活Ca2+信号，对人近端小管细胞发挥保护作用[4]。L-ornithine (1–40mM)降低了原代大鼠脑内皮细胞的细胞阻抗，提高了血脑屏障模型的通透性[5]。
L-ornithine (0.1875, 0.75 or 3mmol/10ml/kg; p.o)可提高小鼠脑内左旋鸟氨酸水平，并具有抗焦虑样作用[6]。全身补充L-ornithine (500 mg/kg; p.o)可提高小鼠排卵期卵巢腐胺水平[7]。
| Cell experiment [1]: | |
Cell lines | HK-2 cells |
Preparation Method | HK-2 cells were transfected with HyperRed (ROS indicator) and treated with/without L-ornithine (l-Orn) for 24h. |
Reaction Conditions | 300μM; 24h |
Applications | L-ornithine has a protective effect against ROS generation in HK-2 cells. |
| Animal experiment [2]: | |
Animal models | Six-week-old male ICR mice |
Preparation Method | Mice were divided into four groups: one control group and three L-ornithine groups. The control group was administered water and the L-ornithine groups were administered L-ornithine monohydrochloride (0.1875, 0.75 or 3mmol/10ml/kg; p.o) dissolved in water. The mice were then given the elevated plus-maze test at 30 minutes post-administration. |
Dosage form | 0.1875, 0.75 or 3mmol/10 ml/kg; p.o |
Applications | L-ornithine in the 0.75mmol group performed best in the elevated plus-maze, a dose that showed some anti-anxiety effects. |
References: | |
| Cas No. | 70-26-8 | SDF | |
| 别名 | 鸟氨酸 | ||
| Canonical SMILES | N[C@@H](CCCN)C(O)=O | ||
| 分子式 | C5H12N2O2 | 分子量 | 132.16 |
| 溶解度 | Water : 50 mg/mL (378.33 mM);DMSO : < 1 mg/mL (insoluble or slightly soluble) | 储存条件 | Store at -20°C, protect from light |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 7.5666 mL | 37.8329 mL | 75.6659 mL |
| 5 mM | 1.5133 mL | 7.5666 mL | 15.1332 mL |
| 10 mM | 0.7567 mL | 3.7833 mL | 7.5666 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
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Quality Control & SDS
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- Purity: >95.00%
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