L-SelenoMethionine
(Synonyms: L-硒代蛋氨酸) 目录号 : GC30266
L-SelenoMethionine是一种硒氨基酸，作为膳食中的天然成分，约占日常硒摄入量的50%以上。
Cas No.:3211-76-5
Sample solution is provided at 25 µL, 10mM.
_1-3.$$$39978408@51.jpg');)
_1-9.$$$38538795@65.jpg');)
_1-9.$$$39112701@51.jpg');)
_1-7.$$$37316672@70.jpg');)
_366-372.$$$36198801@70.jpg');)
.$$$38565142@65.jpg');)
_1867-1883.$$$33248023@67.jpg');)
_eads6055.$$$39977546@45.jpg');)
_eadm9805.$$$40080571@45.jpg');)
_eadj3020.$$$39666821@45.jpg');)
_1-20.$$$39757301@28.jpg');)
_1-24.$$$38898113@28.jpg');)
_273-287.$$$36806353@46.jpg');)
_1-16.$$$37156877@44.jpg');)
_1-19.$$$37460805@44.jpg');)
_1291-1307.$$$34518654@46.jpg');)
L-SelenoMethionine is a selenoamino acid, which is a natural component of the diet and has been estimated to account for at least half of all dietary selenium[1]. L-SelenoMethionine has been widely used as a feed additive for various animals[2]. L-SelenoMethionine has been widely used as a cell viability regulator under oxidative stress conditions[3].
In vitro, L-SelenoMethionine treatment (40µg/ml; 48h) can alleviate the oxidative damage of pulmonary artery smooth muscle cells (PASMCs) under hypoxic conditions and inhibit the proliferation of PASMCs[4]. After treating human thyroid epithelial cells with 5μM L-SelenoMethionine for 24 hours, the radiation effect can be reduced by alleviating stress-related signaling pathways and down-regulating certain genes related to cell adhesion[5]. Treatment with 65μM L-SelenoMethionine for 24 hours significantly inhibited the growth of A549 cells and induced cell apoptosis[6].
In vivo, L-SelenoMethionine treatment (p.o.) at the dose of 12g/g/day for 3 days significantly protected Sprague-Dawley rats from oxidative stress damage induced by space radiation[7]. Oral administration of 0.5mg/kg/day dose of L-SelenoMethionine for one month can effectively alleviate intestinal damage and ferroptosis in sows caused by ammonia exposure[8].
References:
[1] Aguilar F, Charrondiere U R, Dusemund B, et al. L-selenomethionine as a source of selenium added for nutritional purposes to food supplements[J]. EFSA JOURNAL, 2009, 1082: 1-38.
[2] EFSA Panel on Additives and Products or Substances used in Animal Feed (FEEDAP). Scientific Opinion on the safety and efficacy of L‐selenomethionine as feed additive for all animal species[J]. EFSA Journal, 2013, 11(5): 3219.
[3] Sun H, Zhang M, Li J, et al. DL-Selenomethionine alleviates oxidative stress induced by zearalenone via Nrf2/Keap1 signaling pathway in IPEC-J2 cells[J]. Toxins, 2021, 13(8): 557.
[4] Huang T, Liu S, Ma Y, et al. Selenomethionine inhibits the proliferation of hypoxia-induced pulmonary artery smooth muscle cells by inhibiting ROS and HIF-1α-ACE-AngII axis[J]. Scientific Reports, 2025, 15(1): 11746.
[5] Nuth M, Kennedy A R. Mitigating effects of L‑selenomethionine on low‑dose iron ion radiation‑induced changes in gene expression associated with cellular stress[J]. Oncology letters, 2013, 6(1): 35-42.
[6] Redman C, Xu M J, Peng Y M, et al. Involvement of polyamines in selenomethionine induced apoptosis and mitotic alterations in human tumor cells[J]. Carcinogenesis, 1997, 18(6): 1195-1202.
[7] Guan J, Wan X S, Zhou Z, et al. Effects of dietary supplements on space radiation-induced oxidative stress in Sprague-Dawley rats[J]. Radiation research, 2004, 162(5): 572-579.
[8] Zhang X, Wang A, Chen Y, et al. Intestinal barrier dysfunction induced by ammonia exposure in pigs in vivo and in vitro: The protective role of L-selenomethionine[J]. Ecotoxicology and Environmental Safety, 2022, 248: 114325.
L-SelenoMethionine是一种硒氨基酸，作为膳食中的天然成分，约占日常硒摄入量的50%以上[1]。L-SelenoMethionine广泛用作多种动物的饲料添加剂[2]。L-SelenoMethionine在氧化应激条件下作为细胞活力调节剂使用[3]。
在体外，40µg/ml浓度的L-SelenoMethionine处理48小时可缓解缺氧条件下肺动脉平滑肌细胞(PASMCs)的氧化损伤，并抑制细胞增殖[4]。5μM浓度的L-SelenoMethionine处理人甲状腺上皮细胞24小时能通过减轻应激相关信号通路和下调细胞黏附相关基因，降低辐射损伤[5]。65μM浓度的L-SelenoMethionine处理24小时可显著抑制A549细胞生长并诱导细胞凋亡[6]。
在体内，以12μg/g/day的剂量口服L-SelenoMethionine处理3天，能显著保护Sprague-Dawley大鼠免受空间辐射诱导的氧化应激损伤[7]。以0.5mg/kg/day的剂量口服L-SelenoMethionine 处理1个月，可有效缓解氨暴露导致的母猪肠道损伤和铁死亡[8]。
| Cell experiment [1]: | |
Cell lines | Pulmonary artery smooth muscle cells of rat |
Preparation Method | Cell viability was assessed by the CCK-8 assay. Pulmonary artery smooth muscle cells (PASMCs) were plated in 96-well plates at a density of 8×103 cells/well. The medium was replaced after laying 12-16h before the treatment started with L-SelenoMethionine in different concentrations (0, 10, 20, 40, 80, 160, 320, and 640µg/ml). PASMCs were incubated under normoxic (21% O2, 5% CO2) and hypoxic (1% O2, 5% CO2) conditions in incubators for 48h. After incubation with 10µL CCK-8 solution for 1h at 37 °C, the absorbance was detected at 450nm. |
Reaction Conditions | 0, 10, 20, 40, 80, 160, 320, and 640µg/ml; 48h |
Applications | L-SelenoMethionine inhibited the viability of PASMCs in hypoxic conditions in a dose-dependent manner. |
| Animal experiment [2]: | |
Animal models | Large White DurocMin pig |
Preparation Method | Twenty four fattening sows (breed: Large White Duroc Min pig, 125 days of age) with similar body weight were selected and divided randomly into four groups: Control group (C Group, ammonia concentration<5mg/m3, dietary L-SelenoMethionine content of 0.218±0.021mg/kg), L-SelenoMethionine group (Se Group, ammonia concentration<5mg/m3, dietary L-SelenoMethionine content of 0.5mg/kg), ammonia group (A Group, 89.2mg/m3, dietary L-SelenoMethionine 0.218±0.021mg/kg) and ammonia+L-SelenoMethionine group (A+L-SelenoMethionine group, 89.2mg/m3, dietary L-SelenoMethionine content 0.5mg/kg). The Se content of diets was measured by ICP-MS, and the inhalation time of ammonia in the groups A and A+L-SelenoMethionine was 8h. The whole experiment was conducted in the environmental control chamber. The formal test lasted 30 days. The rearing environment met the national standard for fattening pig rearing. All physical parameters were freely adjusted by the environmental control room. During the test period, the animals were fed and free-drinking, and the enclosure was cleaned twice a day. After electric shock anesthesia, all pigs were euthanized by carotid bloodletting at the end of the experiment, and the small intestine samples were quickly collected for analysis of ferroptosis indicators. |
Dosage form | 0.5mg/kg/day for 30 days; p.o. |
Applications | L-SelenoMethionine treatment significantly improved the ferroptosis indicators in the small intestine induced by ammonia. |
References: | |
| Cas No. | 3211-76-5 | SDF | |
| 别名 | L-硒代蛋氨酸 | ||
| Canonical SMILES | O=C(O)[C@@H](N)CC[Se]C | ||
| 分子式 | C5H11NO2Se | 分子量 | 196.11 |
| 溶解度 | Water : 11 mg/mL (56.09 mM) | 储存条件 | Store at -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
||
| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
 |
1 mg | 5 mg | 10 mg |
| 1 mM | 5.0992 mL | 25.4959 mL | 50.9918 mL |
| 5 mM | 1.0198 mL | 5.0992 mL | 10.1984 mL |
| 10 mM | 0.5099 mL | 2.5496 mL | 5.0992 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet