L-Tryptophan-d5
(Synonyms: L-色氨酸-D5,Tryptophan-d5; Tryptophane-d5) 目录号 : GC49370L-色氨酸-d5（L-Tryptophan-d5）是L-色氨酸的氘代化合物，用作GC或LC-MS定量中的标准品。
Cas No.:62595-11-3
Sample solution is provided at 25 µL, 10mM.
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L-Tryptophan-d5 is a deuterated compound of L-tryptophan used as a standard in GC or LC-MS quantification[1]. L-Tryptophan is an essential amino acid and a precursor of serotonin, melatonin, and vitamin B3[2]. L-Tryptophan serves as a substrate for tryptophan hydroxylase (TPH), indoleamine-2,3-dioxygenase (IDO), and tryptophan-2,3-dioxygenase (TDO) for the biosynthesis of serotonin (5-HT) and kynurenine[3]. L-Tryptophan is also metabolized to indole, pyruvate, and ammonia by intestinal bacteria expressing tryptophanase[4].
In vivo, oral administration of L-tryptophan (300mg/kg) to Wistar rats during the day (8:00) increased the levels of 5-HT, 5-hydroxy-L-tryptophan (5-HTP), and 5-hydroxyindole-3-acetic acid (5-HIAA) in the diencephalon of rats. When administered at night (20:00), 5-HT decreased, but the 5-HT/5-HIAA ratio remained unchanged, and melatonin levels and the innate immune response of peritoneal macrophages increased significantly[5].
References:
[1] Kyle J E, Stratton K G, Zink E M, et al. A resource of lipidomics and metabolomics data from individuals with undiagnosed diseases[J]. Scientific data, 2021, 8(1): 114.
[2] Richard D M, Dawes M A, Mathias C W, et al. L-tryptophan: basic metabolic functions, behavioral research and therapeutic indications[J]. International Journal of Tryptophan Research, 2009, 2: IJTR. S2129.
[3] Modoux M, Rolhion N, Mani S, et al. Tryptophan metabolism as a pharmacological target[J]. Trends in pharmacological sciences, 2021, 42(1): 60-73.
[4] Bosi A, Banfi D, Bistoletti M, et al. Tryptophan metabolites along the microbiota-gut-brain axis: an interkingdom communication system influencing the gut in health and disease[J]. International Journal of Tryptophan Research, 2020.
[5]Esteban S, Nicolaus C, Garmundi A, et al. Effect of orally administered L-tryptophan on serotonin, melatonin, and the innate immune response in the rat[J]. Molecular and cellular biochemistry, 2004, 267: 39-46.
| Cas No. | 62595-11-3 | SDF | |
| 别名 | L-色氨酸-D5,Tryptophan-d5; Tryptophane-d5 | ||
| Canonical SMILES | OC([C@@H](N)CC1=C([2H])NC2=C([2H])C([2H])=C([2H])C([2H])=C12)=O | ||
| 分子式 | C11H7D5N2O2 | 分子量 | 209.3 |
| 溶解度 | PBS (pH 7.2): 1 mg/ml | 储存条件 | Store at -20°C, protect from light, stored under nitrogen |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 4.7778 mL | 23.8892 mL | 47.7783 mL |
| 5 mM | 0.9556 mL | 4.7778 mL | 9.5557 mL |
| 10 mM | 0.4778 mL | 2.3889 mL | 4.7778 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet