Lactosylceramide (porcine RBC)
(Synonyms: LacCer) 目录号 : GC52497A sphingolipid
Sample solution is provided at 25 µL, 10mM.
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Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Lactosylceramide (LacCer) is an endogenous bioactive sphingolipid. It is expressed on the plasma membrane of human phagocytes and mediates phagocytosis, chemotaxis, and superoxide generation.1 LacCer forms membrane microdomains with Lyn kinase and the αi subunits of inhibitory G protein-coupled receptors, suggesting a role in cell signaling. Elevated LacCer levels in kidney cortex homogenates and urine are directly correlated with hyperglycemia, insulin resistance, and obesity in db/db transgenic diabetic mice.2 It promotes recruitment of CNS-infiltrating monocytes and microglia and enhances neurodegeneration in mice with chronic experimental autoimmune encephalomyelitis (EAE), a model of multiple sclerosis (MS).3 Increased levels of LacCer in atherosclerotic plaques are correlated with increased levels of the pro-inflammatory cytokines IL-6, monocyte chemoattractant protein-1 (MCP-1), and macrophage inflammatory protein 1β (MIP-1β), as well as lipids and macrophages.4 LacCer is also upregulated during the secretory phase of the menstrual cycle.5 This product contains lactosylceramide molecular species with primarily C22:0, C24:0, and 2-hydroxy C24:0 fatty acyl chain lengths. As this product is derived from a natural source, there may be variations in the sphingoid backbone. [Matreya, LLC. Catalog No. 1500]
1.Iwabuchi, K., Nakayama, H., Oizumi, A., et al.Role of ceramide from glycosphingolipids and its metabolites in immunological and inflammatory responses in humansMediators Inflamm.2015120748(2015) 2.Subathra, M., Korrapati, M., Howell, L.A., et al.Kidney glycosphingolipids are elevated early in diabetic nephropathy and mediate hypertrophy of mesangial cellsAm. J. Physiol. Renal Physiol.309(3)F204-F215(2015) 3.Mayo, L., Trauger, S.A., Blain, M., et al.Regulation of astrocyte activation by glycolipids drives chronic CNS inflammationNat. Med.20(10)1147-1156(2014) 4.Edsfeldt, A., DunÉr, P., StÅhlman, M., et al.Sphingolipids contribute to human atherosclerotic plaque inflammationArterioscler. Thromb. Vasc. Biol.36(6)1132-1140(2016) 5.Mikami, M., Tukazaki, K., Nozawa, S., et al.Menstrual cycle-associated expression of 2-hydroxy fatty acyl phytosphingosine-containing GlcCer, LacCer and Gb3Cer in human uterine endometriumBiochim. Biophys. Acta1125(1)104-109(1992)
| 制备储备液 | |||
 |
1 mg | 5 mg | 10 mg |
| 1 mM | 1.1233 mL | 5.6167 mL | 11.2334 mL |
| 5 mM | 0.2247 mL | 1.1233 mL | 2.2467 mL |
| 10 mM | 0.1123 mL | 0.5617 mL | 1.1233 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。