LAH4 TFA
目录号 : GC62281
LAH4 TFA 是两亲性肽抗生素的 α 螺旋，具有强大的抗菌 (antimicrobial)、核酸转染(nucleic acid transfection) 和细胞渗透活性。LAH4 TFA 具有较高的质粒 DNA 传递能力。LAH4 TFA 对细菌膜外膜中的阴离子脂质有很强的亲和力。
Sample solution is provided at 25 µL, 10mM.
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Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
LAH4 TFA, an alpha-helix of the designed amphipathic peptide antibiotic, exhibits potent antimicrobial, nucleic acid transfection and cell penetration activities. LAH4 TFA possesses high plasmid DNA delivery capacities. LAH4 TFA has a strong affinity for anionic lipids found in the outer membrane of bacterial membranes[1][2][3].
LAH4 TFA is capable of complexing DNA, associating with the cell surfacemembrane and then, when enveloped within an endosome, disrupts the endosomal membrane as the pH drops[1].LAH4 TFA possesses robust plasmid DNA transfection properties. Peptides of the LAH4 family are able to efficiently deliver siRNAs in vitro into a human cell line[2].LAH4 TFA is found to mediate the intracellular delivery of both protein and nucleotide cargo and facilitate protein internalization using mechanisms involving endosomal acidification and processing through the proteasome pathway, leading to enhanced cross presentation of protein antigen by dendritic cells to CD8+ T cells. LAH4 TFA also improves the internalization of CpG, resulting in NF-kB activation, thus potentiating the adjuvant effect of CpG[3]. LAH4 TFA exhibits antibiotic activities against Escherichia coli and Bacillus subtilis. the peptide does not, however, lyse human red blood cells at bacteriocidal concentrations. The antibiotic activities of LAH4 TFA are 2 orders of magnitude more pronounced at pH 5 when compared with pH 7.5[4].
[1]. Mason AJ, et al. The antibiotic and DNA-transfecting peptide LAH4 selectively associates with, and disorders, anionic lipids in mixed membranes. FASEB J. 2006;20(2):320-322.
[2]. Langlet-Bertin B, et al. Design and evaluation of histidine-rich amphipathic peptides for siRNA delivery. Pharm Res. 2010;27(7):1426-1436.
[3]. Zhang TT, et al. LAH4 enhances CD8+ T cell immunity of protein/peptide-based vaccines. Vaccine. 2012;30(4):784-793.
| 制备储备液 | |||
 |
1 mg | 5 mg | 10 mg |
| 1 mM | 0.3456 mL | 1.728 mL | 3.456 mL |
| 5 mM | 0.0691 mL | 0.3456 mL | 0.6912 mL |
| 10 mM | 0.0346 mL | 0.1728 mL | 0.3456 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。