Laminin (925-933)
(Synonyms: H2N-Cys-Asp-Pro-Gly-Tyr-Ile-Gly-Ser-Arg-OH ) 目录号 : GP10103
层粘连蛋白（925-933）（CDPGYIGSR）是层粘连蛋白 B1 链上的序列。
Cas No.:110590-60-8
Sample solution is provided at 25 µL, 10mM.
Laminin (925-933)（CDPGYIGSR）, is the sequence of Laminin on the B1 chain. YIGSR is active in promoting the adhesion of a variety of epithelial cells[1]. Laminin binds to colagen IV, heparan sulfate proteoglycan, and nidogen-entactin,and it mediates cellular interactions with this ma-trix[2].
A laminin-derived synthetic peptide, Cys-Asp-Pro-Gly-Tyr-Ile-Gly-Ser-Arg-NH2 (CDPGYIGSR-H2), containing an active site for cell binding inhibited both angiogenesis and solid tumor growth[3].
In vitro, at concentrations of 100 and 300 µg/ml, Laminin (925-933) can stimulate the attachment of HT-1080 and CHO cells to culture plates[2].
In vitro, the oligopeptide domain Laminin (925-933) covalently linked to an agarose gel as a bioartificial 3D substrate successfully supports neurite outgrowth from dorsal root ganglia (DRG)[2]. In vitro, a 78% reduction in cell attachment was observed on films containing Laminin (925-933) in the cell plating medium[4].
In vivo experiments revealed the capability of a Laminin (925-933)-derivatized gel to enhance nerve regeneration in a transected rat dorsal root model compared to an underivatized gel, a Laminin (925-933) gel, and saline-filled nerve guidance channels[3].
References:
[1] Graf J, et al. A pentapeptide from the laminin B1 chain mediates cell adhesion and binds the 67,000 laminin receptor. Biochemistry. 1987 Nov 3;26(22):6896-900.
[2] Graf J, et al. Identification of an amino acid sequence in laminin mediating cell attachment, chemotaxis, and receptor binding. Cell. 1987 Mar 27;48(6):989-96.
[3] Borkenhagen M, et al. Three-dimensional extracellular matrix engineering in the nervous system. J Biomed Mater Res. 1998 Jun 5;40(3):392-400.
[4] Ranieri JP, et al. Spatial control of neuronal cell attachment and differentiation on covalently patterned laminin oligopeptide substrates. Int J Dev Neurosci. 1994 Dec;12(8):725-35.
层粘连蛋白（925-933）（CDPGYIGSR）是层粘连蛋白 B1 链上的序列。YIGSR 在促进多种上皮细胞粘附方面具有活性[1]。层粘连蛋白能与胶原 IV、硫酸肝素蛋白聚糖和核苷酸-触角蛋白结合，并介导细胞与这些物质的相互作用[2]。
Cys-Asp-Pro-Gly-Tyr-Ile-Gly-Ser-Arg-NH2（CDPGYIGSR-H2）层粘连蛋白的合成肽含有细胞结合的活性位点，可抑制血管生成和实体瘤的生长[3]。
在体外，浓度为 100 和 300 µg/ml 的层粘连蛋白（925-933）可刺激 HT-1080 和 CHO 细胞附着在培养板上[2]。
在体外，寡肽结构域层粘连蛋白（925-933）共价连接到琼脂糖凝胶作为生物人工三维基底，可成功支持背根神经节（DRG）神经元的生长[2]。在体外实验中，在细胞培养介质中含有层粘连蛋白（925-933）的薄膜上观察到细胞附着减少了78%[4]。
体内实验显示，与未充分活化的凝胶、层粘连蛋白（925-933）凝胶和生理盐水填充的神经引导通道相比，层粘连蛋白（925-933）-活化的凝胶能增强横断大鼠背根模型的神经再生能力[3]。
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Laminin coating of culture plates: For coating experiments, all laminin preparations were reconstituted in sterile PBS and then diluted to 10 μg/ml in PBS. Each laminin preparation was adsorbed to 6-well plates for 6 hrs at room temperature. Non-specific binding sites were blocked for 30 minutes with PBS containing 0.1% w/v BSA, at room temperature. Prior to seeding of cells onto the laminin-coated plates, the plates were washed with DMEM. This protocol only provides a guideline, and should be modified according to your specific needs.
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References: [1] Tran T, et al. Endogenous laminin is required for human airway smooth muscle cell maturation. Respir Res. 2006 Sep 12;7(1):117. |
| Cas No. | 110590-60-8 | SDF | |
| 别名 | H2N-Cys-Asp-Pro-Gly-Tyr-Ile-Gly-Ser-Arg-OH | ||
| 化学名 | Laminin (925-933) | ||
| Canonical SMILES | CCC(C)C(C(=O)NCC(=O)NC(CO)C(=O)NC(CCCN=C(N)N)C(=O)O)NC(=O)C(CC1=CC=C(C=C1)O)NC(=O)CNC(=O)C2CCCN2C(=O)C(CC(=O)O)NC(=O)C(CS)N | ||
| 分子式 | C40H62N12O14S | 分子量 | 967.06 |
| 溶解度 | ≥ 48.35mg/mL in DMSO | 储存条件 | Store at -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 1.0341 mL | 5.1703 mL | 10.3406 mL |
| 5 mM | 0.2068 mL | 1.0341 mL | 2.0681 mL |
| 10 mM | 0.1034 mL | 0.517 mL | 1.0341 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet