Lecithin
(Synonyms: 卵磷脂) 目录号 : GC30103
A sphingolipid
Cas No.:8002-43-5
Sample solution is provided at 25 µL, 10mM.
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Phosphatidylcholine is the most abundant phospholipid in mammalian, plant, and yeast cells.1 It is found mainly in the outer leaflet of cell membranes and can make up approximately half of the total phospholipids.2 In mammalian tissues, phosphatidylcholine commonly contains a saturated and unsaturated fatty acid at the C-1 and C-2 positions of glycerol, respectively. It is a substrate for various enzymes in cell signaling pathways that is cleaved by phospholipases into diacylglycerol and phosphocholine or phosphatidic acid and choline. This product contains glucosylceramide molecular species with primarily C16:0, C22:0, and C24:0 fatty acyl chain lengths. As this product is derived from a natural source, there may be variations in the sphingoid backbone. [Matreya, LLC. Catalog No. 1044]
1.Vance, J.E.Phosphatidylserine and phosphatidylethanolamine in mammalian cells: Two metabolically related aminophospholipidsJ. Lipid Res.49(7)1377-1387(2008) 2.Billah, M.M., and Anthes, J.C.The regulation and cellular functions of phosphatidylcholine hydrolysisBiochem J.269(2)281-291(1990)
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Cell experiment: |
MRS broths are supplemented with soy Lecithin concentrations of 0, 0.2, 0.4, 0.6, 0.8 and 1.0%. Each broth is inoculated with a tested strain culture (2%, v/v) and anaerobically incubated at 37°C for 20 h. After incubation, the bacterium cells are harvested by centrifugation at 8000 g for 10 min at 4°C and washed twice in PBS (pH 6.5) plus ethanol (5%, v/v). Strain bile resistance is assessed. The numbers of viable cells are counted by the pouring plate method, and each batch is tested three times[1]. |
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References: [1]. Hu B, et al. Enhancement of bile resistance in Lactobacillus plantarum strains by soy lecithin. Lett Appl Microbiol. 2015 Jul;61(1):13-9. |
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| Cas No. | 8002-43-5 | SDF | |
| 别名 | 卵磷脂 | ||
| Canonical SMILES | O=P(OC[C@@H](COC(CCCCCCCCCCCCCCC)=O)OC(CCCCCCC/C=C\C/C=C\CCCCC)=O)(OCC[N+](C)(C)C)[O-] | ||
| 分子式 | C42H80NO8P | 分子量 | 758.06 |
| 溶解度 | DMSO : 5 mg/mL (6.60 mM);Water : 3.33 mg/mL (4.39 mM) | 储存条件 | Store at 2-8°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 1.3192 mL | 6.5958 mL | 13.1916 mL |
| 5 mM | 0.2638 mL | 1.3192 mL | 2.6383 mL |
| 10 mM | 0.1319 mL | 0.6596 mL | 1.3192 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Quality Control & SDS
- View current batch:
- Purity: >98.50%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet