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Leptin Human, His

目录号 : GP20687

Leptin Human Recombinant, His Tag

Leptin Human, His Chemical Structure

规格 价格 库存 购买数量
5μg
¥840.00
5-10工作日
25μg
¥2,030.00
5-10工作日
1mg
¥33,600.00
5-10工作日

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Sample solution is provided at 25 µL, 10mM.

Description

Leptin Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing amino acids 48-167 and having a total molecular mass of 19 kDa including the 4 kDa His tag.The Leptin is purified by proprietary chromatographic techniques.

Product Data

Purity Greater than 90.0% as determined by(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE. Source Escherichia Coli.
Phycical Appearance Sterile Filtered White lyophilized (freeze-dried) powder. Shipping Condition Shipped at Room temp.
Synonyms OB Protein; Obesity Protein; OBS; Obesity factor.
Solubility The lyophilized Leptin is very soluble in water and most aqueous buffers below and above the isoelectric point.
Stability Lyophilized Leptin although stable at room temperature, should be stored desiccated below 0°C . Reconstituted Leptin is best stored refrigerated at 4°C .Please avoid freeze-thaw cycles.
Formulation The protein was lyophilized from a concentrated (1mg/ml) solution with 1X PBS, 0.1% SDS and 1mM DTT.

Introduction

Leptin is a protein hormone with important effects in regulating body weight, metabolism and reproductive function. The protein is approximately~16 kDa in mass and encoded by the obese (ob)gene. leptin is expressed predominantly by adipocytes, which fits with the idea that body weight is sensed as the total mass of fat in the body. Smaller amounts of leptin are also secreted by cellsin the epithelium of the stomach and in the placenta. Leptin receptors are highly expressed in areas of the hypothalamus known to be important in regulating body weight, as well as in T lymphocytes and vascular endothelial cells.

Applications

1. Positive control for Western blot.2. Antibody production. 3. Protein assay.

Stability

Lyophilized Leptin although stable at room temperature, should be stored desiccated below 0°C . Reconstituted Leptin is best stored refrigerated at 4°C .Please avoid freeze-thaw cycles.

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