Leptin Human, N82K
目录号 : GP20688Leptin Human Recombinant, N82K
Sample solution is provided at 25 µL, 10mM.
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| Purity | Greater than 98.0% as determined by:(a)Gel filtration analysis.(b) Analysis by SDS-PAGE. | Source | Escherichia Coli. |
| Phycical Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. | Shipping Condition | Shipped at Room temp. |
| Synonyms | OB Protein; Obesity Protein; OBS; Obesity factor. | ||
| Amino Acid Sequence | The sequence of the first five N-terminal amino acids was determined and was found to be Ala-Val-Pro-Ile-Gln. | ||
| Solubility | It is recommended to reconstitute the lyophilized leptin N82K in sterile water or 0.4% NaHCO3 adjusted to pH 8-9, not less than 100µg/ml, which can then be further diluted to other aqueous solutions. | ||
| Stability | Lyophilized leptin N82K although stable at room temperature for 3 weeks, should be stored desiccated below -18°C . Upon reconstitution leptin N82K should be stored at 4°C between 2-7 days and for future use below -18°C . For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles. | ||
| Biological Activity | Biological Activity is < than 0.1% as determined by inducing proliferation of BAF/3 cells stably transfected with the long form of human leptin receptor. | ||
| Formulation | Lyophilized from a concentrated (1mg/ml) solution with 0.0045mM NaHCO3. | ||
Leptin takes an important part in the regulation of energy balance and body weight control.After entering the circulation, Leptin binds LEPRwhich results in the activation of several major signalling pathways. In the hypothalamus Leptin acts as an appetite-regulating factor that induces a decrease in food intake and an increase in energy consumption and also regulates bone mass and secretion of hypothalamo-pituitary-adrenal hormones. In the periphery, increases basal metabolism, regulates pancreatic beta-cell function and insulin secretion and affects innate and adaptive immunity.
Biological Activity is < than 0.1% as determined by inducing proliferation of BAF/3 cells stably transfected with the long form of human leptin receptor.
Lyophilized leptin N82K although stable at room temperature for 3 weeks, should be stored desiccated below -18°C . Upon reconstitution leptin N82K should be stored at 4°C between 2-7 days and for future use below -18°C . For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.