Leptin qA Ovine
目录号 : GP20715Leptin Antagonist Quadruple Mutant Ovine Recombinant
Sample solution is provided at 25 µL, 10mM.
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| Purity | Greater than 98.0% as determined by:(a) Gel filtration analysis.(b) Analysis by SDS-PAGE. | Source | Escherichia coli. |
| Phycical Appearance | White lyophilized (freeze-dried) powder. | Shipping Condition | Shipped at Room temp. |
| Amino Acid Sequence | The sequence of the first five N-terminal amino acids was determined and was found to be Ala-Val-Pro-Ile-Arg. | ||
| Solubility | It is recommended to reconstitute the lyophilized Leptin-Antagonist Quadruple Mutant Ovine Recombinant in sterile 0.4% NaHCO3 adjusted to pH 8-9, not less than 100µg/ml, which can then be further diluted to other aqueous solutions. | ||
| Stability | Lyophilized Leptin-Antagonist Quadruple Mutant Ovine Recombinant although stable at room temperature for several weeks, should be stored desiccated below -18°C . Upon reconstitution at > 0.1 LEP-qA mutant mg/ml and up to 2mg/ml and filter sterilization Leptin mutant can be stored at 4°C or even room temperature for several weeks making it suitable for long term infusion studies using osmotic pumps. At lower concentration addition of a carrier protein (0.1% HSA or BSA) is suggested. Please prevent freeze-thaw cycles. | ||
| Biological Activity | ProSpecбпs leptin qA is capable of inhibiting leptin-induced proliferation of BAF/3 cells stably transfected with the long form of ovine leptin receptor. It also inhibits various leptin effects in several in vitro bioassays. | ||
| Formulation | The protein was lyophilized from a concentrated (1mg/ml) solution with 0.0045mM NaHCO3. | ||
ProSpecбпs leptin qA is capable of inhibiting leptin-induced proliferation of BAF/3 cells stably transfected with the long form of ovine leptin receptor. It also inhibits various leptin effects in several in vitro bioassays.
Lyophilized Leptin-Antagonist Quadruple Mutant Ovine Recombinant although stable at room temperature for several weeks, should be stored desiccated below -18°C . Upon reconstitution at > 0.1 LEP-qA mutant mg/ml and up to 2mg/ml and filter sterilization Leptin mutant can be stored at 4°C or even room temperature for several weeks making it suitable for long term infusion studies using osmotic pumps. At lower concentration addition of a carrier protein (0.1% HSA or BSA) is suggested. Please prevent freeze-thaw cycles.