Leukotriene B4-d4

Leukotriene B₄-d₄ (LTB₄-d₄) contains four deuterium atoms at the 6, 7, 14, and 15 positions. It is intended for use as an internal standard for the quantification of LTB₄ by GC- or LC-mass spectrometry. LTB₄ is a dihydroxy fatty acid derived from arachidonic acid through the 5-lipoxygenase pathway.^1,2,3 It promotes a number of leukocyte functions including aggregation, stimulation of ion fluxes, enhancement of lysosomal enzyme release, superoxide anion production, chemotaxis, and chemokinesis. In subnanomolar ranges (3.9 x 10^-10 M), LTB₄ causes chemotaxis and chemokinesis in human PMNL.⁴ At higher concentrations, (1.0 x 10^-7 M), LTB₄ leads to neutrophil aggregation and degranulation as well as superoxide anion production.^4,5

1.RÅdmark, O., Malmsten, C., Samuelsson, B., et al.Leukotriene A: Stereochemistry and enzymatic conversion to leukotriene BBiochem. Biophys. Res. Commun.92(3)954-961(1980) 2.Ford-Hutchinson, A.W., Bray, M.A., Doig, M.V., et al.Leukotriene B, a potent chemokinetic and aggregating substance released from polymorphonuclear leukocytesNature286(5770)264-265(1980) 3.McGee, J., and Fitzpatrick, F.Enzymatic hydration of leukotriene A4. Purification and characterization of a novel epoxide hydrolase from human erythrocytesJ. Biol. Chem.260(23)12832-12837(1985) 4.Ford-Hutchinson, A.W.Leukotriene B4 in inflammationCrit. Rev. Immunol.10(1)1-12(1990) 5.McMillan, R.M., and Foster, S.J.Leukotriene B4 and inflammatory diseaseAgents Actions24(1-2)114-119(1988)