Leukotriene B4-d5
(Synonyms: LTB4-d5) 目录号 : GC49597
An internal standard for the quantification of LTB4
Sample solution is provided at 25 µL, 10mM.
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Quality Control & SDS
- View current batch:
- Purity: >99.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Leukotriene B4-d5 (LTB4-d5) is intended for use as an internal standard for the quantification of LTB4 by GC- or LC-MS. LTB4 is a dihydroxy fatty acid derived from arachidonic acid through the 5-lipoxygenase (5-LO) pathway.1,2,3 It promotes a number of leukocyte functions including aggregation, stimulation of ion fluxes, enhancement of lysosomal enzyme release, superoxide anion production, chemotaxis, and chemokinesis. In subnanomolar ranges (3.9 x 10-10 M), LTB4 induces chemotaxis and chemokinesis in human polymorphonuclear leukocytes.4 At higher concentrations, (1.0 x 10-7 M), LTB4 leads to neutrophil aggregation and degranulation as well as superoxide anion production.4,5
1.RÅdmark, O., Malmsten, C., Samuelsson, B., et al.Leukotriene A: Stereochemistry and enzymatic conversion to leukotriene BBiochem. Biophys. Res. Commun.92(3)954-961(1980) 2.Ford-Hutchinson, A.W., Bray, M.A., Doig, M.V., et al.Leukotriene B, a potent chemokinetic and aggregating substance released from polymorphonuclear leukocytesNature286(5770)264-265(1980) 3.McGee, J., and Fitzpatrick, F.Enzymatic hydration of leukotriene A4. Purification and characterization of a novel epoxide hydrolase from human erythrocytesJ. Biol. Chem.260(23)12832-12837(1985) 4.Ford-Hutchinson, A.W.Leukotriene B4 in inflammationCrit. Rev. Immunol.10(1)1-12(1990) 5.McMillan, R.M., and Foster, S.J.Leukotriene B4 and inflammatory diseaseAgents Actions24(1-2)114-119(1988)
| Cas No. | N/A | SDF | Download SDF |
| 别名 | LTB4-d5 | ||
| Canonical SMILES | O[C@H](C/C=C\CCCC([2H])([2H])C([2H])([2H])[2H])/C=C/C=C/C=C\[C@@H](O)CCCC(O)=O | ||
| 分子式 | C20H27D5O4 | 分子量 | 341.5 |
| 溶解度 | DMF: 50mg/mL,DMSO: 50mg/mL,Ethanol: 50mg/mL,PBS (pH 7.2): 1mg/mL | 储存条件 | -80°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 2.9283 mL | 14.6413 mL | 29.2826 mL |
| 5 mM | 0.5857 mL | 2.9283 mL | 5.8565 mL |
| 10 mM | 0.2928 mL | 1.4641 mL | 2.9283 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。