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Leukotriene C4 methyl ester Sale

(Synonyms: LTC4 methyl ester) 目录号 : GC44054

A more lipid soluble formulation of LTE4

Leukotriene C4 methyl ester Chemical Structure

Cas No.:73958-10-8

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25μg
¥2,021.00
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50μg
¥3,838.00
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100μg
¥7,281.00
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Sample solution is provided at 25 µL, 10mM.

产品文档

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产品描述

Leukotriene C4 (LTC4) is the parent cysteinyl-leukotriene produced by the LTC4 synthase-catalyzed conjugation of glutathione to LTA4. LTC4 is produced by neutrophils, macrophages, and mast cells, and by transcellular metabolism in platelets. It is one of the constituents of slow-reacting substance of anaphylaxis (SRS-A) and exhibits potent smooth muscle contracting activity. LTC4-induced bronchoconstriction and enhanced vascular permeability contribute to the pathogenesis of asthma and acute allergic hypersensitivity. The concentration of LTC4 required to produce marked contractions of lung parenchymal strips and isolated tracheal rings is about 1 nM. LTC4 methyl ester is a more lipid soluble form of LTC4. The biological activity of LTC4 methyl ester has not been reported.

Chemical Properties

Cas No. 73958-10-8 SDF
别名 LTC4 methyl ester
Canonical SMILES CCCCC/C=C\C\C=C/C=C\C=C/C(SCC(N)(C(=O)NCC(=O)O)N1C(=O)CCC1C(=O)O)[C@@H](O)CCCC(=O)OC
分子式 C31H49N3O9S 分子量 639.8
溶解度 DMF: 50 mg/ml,DMSO: 50 mg/ml,Ethanol: 1 mg/ml,PBS (pH 7.2): .15 mg/ml 储存条件 Store at -20°C
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储备液的保存方式和期限:-80°C 储存时,请在 6 个月内使用,-20°C 储存时,请在 1 个月内使用。
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溶解性数据

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1 mg 5 mg 10 mg
1 mM 1.563 mL 7.8149 mL 15.6299 mL
5 mM 0.3126 mL 1.563 mL 3.126 mL
10 mM 0.1563 mL 0.7815 mL 1.563 mL
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Research Update

Racemic LTA4 methyl ester bioconversion into LTC4 methyl ester by various glutathione S-transferases

Biochem Int 1987 Dec;15(6):1127-35.PMID:3125835doi

It has been shown that various glutathione transferases can synthesize leukotriene C4, or its methyl ester, from glutathione and leukotriene A4. We questioned whether the same enzymes could be used to resolve racemic leukotriene A4 methyl ester (more easily prepared than the optically active enantiomer) and to produce Leukotriene C4 methyl ester selectively. We present in this paper a study of the enantioselectivity of some rat liver glutathione transferase isozymes and of the glutathione transferase of human placenta for the leukotriene A4 methyl ester isomers. The rat liver 3-4 glutathione transferase exhibited the highest conversion rate but preferentially converted the (5R, 6R) leukotriene A4 methyl ester. The placental enzyme was fairly selective for the natural (5S, 6S) enantiomer but the rate of conversion was low.

Leukotriene C4 synthesis catalyzed by Dirofilaria immitis glutathione S-transferase

Am J Trop Med Hyg 1989 Feb;40(2):171-5.PMID:2493201DOI:10.4269/ajtmh.1989.40.171.

The biologically active sulfidopeptide leukotriene, leukotriene C4, is formed by the enzymatic action of leukotriene C4 synthase, which conjugates glutathione with leukotriene A4. We have found that a filarial glutathione S-transferase can function as a leukotriene C4 synthase. Glutathione S-transferase was purified from the cytosol of adult Dirofilaria immitis by glutathione-agarose affinity chromatography and was reacted with 25 microM leukotriene A4 methyl ester and 10 mM glutathione. The filarial enzyme catalyzed the formation of Leukotriene C4 methyl ester, as shown by reverse phase high pressure liquid chromatographic analyses. The finding that filarial glutathione S-transferase can function as leukotriene C4 synthase provides a mechanism whereby filarial parasites could form lipoxygenase pathway derived sulfidopeptide leukotrienes.