Lipid peroxidation inhibitor 1
(Synonyms: 脂质过氧化抑制剂1) 目录号 : GC31275
Lipidperoxidationinhibitor1是一种脂质过氧化(lipidperoxidation)抑制剂，其IC50值为0.07μM。
Cas No.:142873-41-4
Sample solution is provided at 25 µL, 10mM.
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Lipid peroxidation inhibitor 1 is a lipid peroxidation inhibitor with an IC50 of 0.07 μM.
Among the compounds synthesized, Lipid peroxidation inhibitor 1 (26n) exhibits potent effects in the inhibition of lipid peroxidation, with an IC50 of 0.07 μM[1].
Basic molecules 8a and 27 exhibit significant effects against (methamphetamine) MAP-induced hypermotility. Except in these compounds, a phenyl group adjacent to the methyl group in position 2 at a distance seems to be indispensable (compare compounds 26m, o, p with compounds Lipid peroxidation inhibitor 1 (26n), q, r). Among these compounds, Lipid peroxidation inhibitor 1 which has a phenylpiperidinyl group in position 2 is found to be most effective[1].
[1]. Ohkawa S, et al. 5-aminocoumarans: dual inhibitors of lipid peroxidation and dopamine release with protectiveeffects against central nervous system trauma and ischemia. J Med Chem. 1997 Feb 14;40(4):559-73.
Cell experiment: | To rat liver microsomes (S-9) (0.3 mg of protein/40 mM Tris-malate buffer (pH 7.4), 2.4 mL) (2.4 mL) is added a mixture (1:1; 0.1 mL) of aqueous FeCl2 solution (0.25 mM) and NADPH (3 mM). After incubation of the homogenate for 1 h at 37 °C, peroxide production is determined by the thiobarbituric acid method. The inhibitory activities on lipid peroxidation are expressed as IC50 values or percent inhibition as compare with the amount of production in the vehicle (DMSO) group[1]. |
Animal experiment: | Five-week old male mice (25 to 35 g) are used. Following a 90 min acclimation period, the test compounds (including Lipid peroxidation inhibitor 1) suspended in 5% gum arabic are injected intraperitoneally in a volume of 20 mL/kg. Thirty minutes after treatment with test compounds, methamphetamine dissolved in saline is injected intraperitoneally at a dose of 1 mg/kg in a volume of 20 mL/kg. Immediately after methamphetamine injection, spontaneous motor activity is monitored for 90 min[1]. |
References: [1]. Ohkawa S, et al. 5-aminocoumarans: dual inhibitors of lipid peroxidation and dopamine release with protectiveeffects against central nervous system trauma and ischemia. J Med Chem. 1997 Feb 14;40(4):559-73. | |
| Cas No. | 142873-41-4 | SDF | |
| 别名 | 脂质过氧化抑制剂1 | ||
| Canonical SMILES | NC1=C(C)C(C)=C(OC(CN2CCC(C3=CC=CC=C3)CC2)(C)C4)C4=C1C | ||
| 分子式 | C24H32N2O | 分子量 | 364.52 |
| 溶解度 | Soluble in DMSO | 储存条件 | Store at -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 2.7433 mL | 13.7167 mL | 27.4333 mL |
| 5 mM | 0.5487 mL | 2.7433 mL | 5.4867 mL |
| 10 mM | 0.2743 mL | 1.3717 mL | 2.7433 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet