LipoRNAiMAX Transfection Reagent
目录号 : GK20007LipoRNAiMAX转染试剂可以在多种细胞类型中获得最高的转染效率，并且适用于siRNA介导的基因抑制实验。
Sample solution is provided at 25 µL, 10mM.
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LipoRNAiMAX Transfection Reagentcan obtain the highest transfection efficiency in a variety of cell types, and is suitable for siRNA-mediated gene suppression experiments. LipoRNAiMAX is a unique RNAi-specific cationic liposome transfection reagent, suitable for delivering siRNA and miRNA to all cell types.
- Ultra-high transfection efficiency, and low RNAi concentration required, better gene suppression effect, few non-specific effects.
- Low cytotoxicity, easy to optimize, showing low cytotoxicity across the 10-fold transfection reagent concentration range.
- Excellent transfection efficiency for miRNA antagonists and mimics.
- It can be used in a variety of cell types and is the most versatile method suitable for various gene suppression experiments.
- Simple and fast experimental protocol to obtain stable and reproducible results.
LipoRNAiMAX Transfection Reagent可在多种细胞类型中获得最高的转染效率，适用于siRNA介导的基因抑制实验。 LipoRNAiMAX 是一种独特的 RNAi 特异性阳离子脂质体转染试剂，适用于将 siRNA 和 miRNA 递送至所有细胞类型。
- 超高转染效率，所需RNAi浓度低，基因抑制效果好，非特异性效应少。
- 低细胞毒性，易于优化，在10倍转染试剂浓度范围内表现出低细胞毒性。
- 对 miRNA 拮抗剂和模拟物具有出色的转染效率。
- 可用于多种细胞类型，是适用于各种基因抑制实验的最通用的方法。
- 简单快速的实验方案，以获得稳定和可重复的结果。
Preparation Before Experiment
• siRNA or miRNA (10 μM stock)
• Opti-MEM Reduced Serum Medium
• Microcentrifuge tube
Experimental Steps
A. LipoRNAiMAX Transfection Reagent requirements
| Component | 96-well | 24-well | 6-well |
| Final siRNA per well | 1 pmol | 5 pmol | 25 pmol |
| Final LipoRNAiMAX Reagent per well | 0.3 μL | 1.5 μL | 7.5 μL |
B. Transfection of siRNA
Reverse transfection is faster to perform than forward transfection and is themethod of choice for high-throughput transfection. Perform reverse transfection by preparing complexes inside the wells,and then adding cells and medium. Because the cells and siRNA-lipid complexes are prepared on the same day, we recommended using 2.5× more cells than for a regular transfection method.
C. LipoRNAiMAX Transfection Reagent Protocol
Transfect cells according to the following table.The transfection is designed for one RNA amount combined with one amount of LipoRNAiMAX. The prepared mix is enough to have triplicates (96-well), duplicates (24-well), and single well (6-well) transfections, and account for pipetting variations. For additional information on scaling your transfection reaction.
| Components | 0.25 mL | 0.5 mL |
| LipoRNAiMAX Transfection Reagent | 0.25 mL | 0.5 mL |
| Store at 4°C. Do not freeze. | ||
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