Lucidin
(Synonyms: 卢西定; NSC 30546) 目录号 : GC14424
Lucidin (NSC 30546) 是 Rubia tinctorum L 的天然成分。
Cas No.:478-08-0
Sample solution is provided at 25 µL, 10mM.
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Lucidin (NSC 30546) is a natural component of Rubia tinctorum L., which is mutagenic in bacteria and mammalian cells [1].
Hydroxyanthraquinones (HAs) are special organic compounds widely distributed in the plant kingdom. The HA plants have been used as laxatives and colorant for thousands of years. In the last century, HAs were also employed as medicine for the treatment of kidney stone. Lucidin is a HA extracted from the root of Rubia tinctorum L. that also known as madder. It was thought to be genotoxic in bacteria and mammalian cells [1].
Lucidin was found to be mutagenic in five Salmonella typhimurium strains without metabolic activation. Additionally, in Chinese hamster fibroblast V79 cells, lucidin was found to be mutagenic at the hypoxanthine-guanine phosphoribosyl transferase gene locus, which induced DNA single-strand breaks and DNA-protein cross-link. In primary rat hepatocytes and transformed C3H/ M2-mouse fibroblasts, it was observed lucidin might induce DNA repair synthesis [1].
In mouse model, when ACI rats were treated with 1-10% madder roots in the diet and control group without madder roots for 780 days, the dose-dependent increase of benign and malignant tumors were observed in liver and kidney. Additionally, DNA adducts were observed in liver, kidney and colon when treated with 10% madder root for two weeks. The formation of DNA adducts and mutagenicity was thought to be associated with lucidin which was contained in the madder roots [2].
References:
[1] Westendorf J et al. , The genotoxicity of lucidin, a natural component of Rubia tinctorum L., and lucidinethylether, a component of ethanolic Rubia extracts. Cell Biol Toxicol. 1988, 4(2):225-239.
[2] Westendorf J et al. , Carcinogenicity and DNA adduct formation observed in ACI rats after long-term treatment with madder root, Rubia tinctorum L. Carcinogenesis. 1998, 19(12):2163-2168.
| Cell experiment: [1] | |
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Cell lines |
PANC-1 cells |
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Preparation method |
The solubility of this compound in DMSO is >10 mM. General tips for obtaining a higher concentration: Please warm the tube at 37 °C for 10 minutes and/or shake it in the ultrasonic bath for a while.Stock solution can be stored below -20°C for several months. |
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Reaction Conditions |
PC50: 54.7 μM, 24 hours |
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Applications |
After 24 h of incubation, the cells were washed with PBS, and 100 μL of DMEM containing 10% WST-8 cell counting kit solution was added to the wells. After 3 h of incubation, the absorbance was measured at 450 nm. The preferential cytotoxicity was expressed as the concentration at which 50% of cells died preferentially. The PC50 value of lucidin was 54.7 μM in this experiment. |
| Animal experiment: [2] | |
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Animal models |
Male Parkes mice |
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Dosage form |
Oral administration, 2 mg/day |
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Applications |
Mice were treated orally with lucidin for 4 days. 32P-Postlabelling analysis of hepatic DNA from mice treated with lucidin showed three lucidin-caused radioactive spots not present in the chromatograms of DNA from the control animals. The adduct levels calculated from individual animals were 1.16 ± 0.2 total adducts/108 nucleotides. |
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Other notes |
Please test the solubility of all compounds indoor, and the actual solubility may slightly differ with the theoretical value. This is caused by an experimental system error and it is normal. |
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References: [1] Dibwe D F, Awale S, Kadota S, et al. Damnacanthal from the Congolese Medicinal Plant Garcinia huillensis has a Potent Preferential Cytotoxicity against Human Pancreatic Cancer PANC-1 Cells. Phytotherapy Research, 2012, 26(12): 1920-1926. [2] Poginsky B, Westendorf J, Blömeke B, et al. Evaluation of DNA-binding activity of hydroxyanthraquinones occurring in Rubia tinctorum L. Carcinogenesis, 1991, 12(7): 1265-1271. | |
| Cas No. | 478-08-0 | SDF | |
| 别名 | 卢西定; NSC 30546 | ||
| 化学名 | 1,3-dihydroxy-2-(hydroxymethyl)anthracene-9,10-dione | ||
| Canonical SMILES | C1=CC=C2C(=C1)C(=O)C3=CC(=C(C(=C3C2=O)O)CO)O | ||
| 分子式 | C15H10O5 | 分子量 | 270.24 |
| 溶解度 | Soluble in DMSO | 储存条件 | Store at -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 3.7004 mL | 18.5021 mL | 37.0041 mL |
| 5 mM | 0.7401 mL | 3.7004 mL | 7.4008 mL |
| 10 mM | 0.37 mL | 1.8502 mL | 3.7004 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet