Lumogallion
(Synonyms: 荧光镓) 目录号 : GC49604
A fluorescent probe for aluminum
Cas No.:4386-25-8
Sample solution is provided at 25 µL, 10mM.
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Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Lumogallion is a fluorescent probe for aluminum.1 It binds to aluminum, forming a complex that displays excitation/emission maxima of 500/590 nm, respectively. Lumogallin has been used to detect aluminum in natural waters and human urine and serum samples by HPLC, as well as in fixed soybean roots and THP-1 cells by confocal microscopy.2,3,4,1
1.Mold, M., Eriksson, H., SiesjÖ, P., et al.Unequivocal identification of intracellular aluminium adjuvant in a monocytic THP-1 cell lineSci. Rep.46287(2014) 2.Sutheimer, S.H., and Cabaniss, S.E.Determination of trace aluminum in natural waters by flow-injection analysis with fluorescent detection of the lumogallion complexAnalytica Chimica Acta303211-221(1995) 3.Harper, M., Glowacki, C.R., and Michael, P.R.Industrial hygieneAnal. Chem.69(12)307R-327R(1997) 4.Silva, I.R., Smyth, T.J., Moxley, D.F., et al.Aluminum accumulation at nuclei of cells in the root tip. Fluorescence detection using lumogallion and confocal laser scanning microscopyPlant Physiol.123(2)543-552(2000)
| Cas No. | 4386-25-8 | SDF | Download SDF |
| 别名 | 荧光镓 | ||
| Canonical SMILES | O=S(O)(C1=CC(Cl)=CC(/N=N/C2=C(O)C=C(O)C=C2)=C1O)=O | ||
| 分子式 | C12H9ClN2O6S | 分子量 | 344.7 |
| 溶解度 | DMF: 10 mg/ml,DMSO: 10 mg/ml,Ethanol: 1 mg/ml,PBS (pH 7.2): insol | 储存条件 | -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 2.9011 mL | 14.5054 mL | 29.0107 mL |
| 5 mM | 0.5802 mL | 2.9011 mL | 5.8021 mL |
| 10 mM | 0.2901 mL | 1.4505 mL | 2.9011 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Self-plasticized, lumogallion-based fluorescent optical sensor for the determination of aluminium (III) with ultra-low detection limits
Anal Chim Acta 2020 Mar 8;1101:141-148.PMID:32029105DOI:10.1016/j.aca.2019.12.021.
Aluminium-selective ion optical sensor based on covalently attached Lumogallion methacrylate was synthesized and investigated in this study. Lumogallion based derivatives were copolymerized with various methacrylate monomers via a simple one step free radical polymerization to produce a "self-plasticized" copolymer. We demonstrate that covalently attached Lumogallion moieties provide adequate functionality to the optical film thus achieving a very simple, one component sensing membrane. Fluorescence experiments demonstrated excellent sensitivity towards aluminium (III) ions with the detection limits found at 4.8 × 10-12 M. Furthermore, proposed sensor displays high selectivity towards aluminium over a number of biologically relevant cations. Moreover, the synthesized copolymer was used for the fabrication of nanoparticles that exhibit strong fluorescence upon contact with aluminium (III) ions. It is anticipated that lumogallion-based copolymers may form the basis for the development of highly sensitive and robust aluminium selective sensors capable of in situ measurements.
Al adjuvants can be tracked in viable cells by Lumogallion staining
J Immunol Methods 2015 Jul;422:87-94.PMID:25896212DOI:10.1016/j.jim.2015.04.008.
The mechanism behind the adjuvant effect of aluminum salts is poorly understood notwithstanding that aluminum salts have been used for decades in clinical vaccines. In an aqueous environment and at a nearly neutral pH, the aluminum salts form particulate aggregates, and one plausible explanation of the lack of information regarding the mechanisms could be the absence of an efficient method of tracking phagocytosed aluminum adjuvants and thereby the intracellular location of the adjuvant. In this paper, we want to report upon the use of Lumogallion staining enabling the detection of phagocytosed aluminum adjuvants inside viable cells. Including micromolar concentrations of Lumogallion in the culture medium resulted in a strong fluorescence signal from cells that had phagocytosed the aluminum adjuvant. The fluorescence appeared as spots in the cytoplasm and by confocal microscopy and co-staining with probes presenting fluorescence in the far-red region of the spectrum, aluminum adjuvants could to a certain extent be identified as localized in acidic vesicles, i.e., lysosomes. Staining and detection of intracellular aluminum adjuvants was achieved not only by diffusion of Lumogallion into the cytoplasm, thereby highlighting the presence of the adjuvant, but also by pre-staining the aluminum adjuvant prior to incubation with cells. Pre-staining of aluminum adjuvants resulted in bright fluorescent particulate aggregates that remained fluorescent for weeks and with only a minor reduction of fluorescence upon extensive washing or incubation with cells. Both aluminum oxyhydroxide and aluminum hydroxyphosphate, two of the most commonly used aluminum adjuvants in clinical vaccines, could be pre-stained with Lumogallion and were easily tracked intracellularly after incubation with phagocytosing cells. Staining of viable cells using Lumogallion will be a useful method in investigations of the mechanisms behind aluminum adjuvants' differentiation of antigen-presenting cells into inflammatory cells. Information will be gained regarding the phagosomal pathways and the events inside the phagosomes, and thereby the ultimate fate of phagocytosed aluminum adjuvants could be resolved.
The Identification of Aluminum in Human Brain Tissue Using Lumogallion and Fluorescence Microscopy
J Alzheimers Dis 2016 Oct 18;54(4):1333-1338.PMID:27472886DOI:10.3233/JAD-160648.
Aluminum in human brain tissue is implicated in the etiologies of neurodegenerative diseases including Alzheimer's disease. While methods for the accurate and precise measurement of aluminum in human brain tissue are widely acknowledged, the same cannot be said for the visualization of aluminum. Herein we have used transversely-heated graphite furnace atomic absorption spectrometry to measure aluminum in the brain of a donor with Alzheimer's disease, and we have developed and validated fluorescence microscopy and the fluor Lumogallion to show the presence of aluminum in the same tissue. Aluminum is observed as characteristic orange fluorescence that is neither reproduced by other metals nor explained by autofluorescence. This new and relatively simple method to visualize aluminum in human brain tissue should enable more rigorous testing of the aluminum hypothesis of Alzheimer's disease (and other neurological conditions) in the future.
Fully-automated fluorimetric determination of aluminum in seawater by in-syringe dispersive liquid-liquid microextraction using Lumogallion
Anal Chem 2012 Nov 6;84(21):9462-9.PMID:23005696DOI:10.1021/ac302083d.
A sensitive and selective automated in-syringe dispersive liquid-liquid microextraction (DLLME) method is presented. It was successfully applied to the determination of aluminum in coastal seawater samples. The complete analytical procedure including sampling, buffering, reaction of the analyte with fluorescence reagent Lumogallion (LMG), extraction, phase separation, and quantification was completely automized and carried out within 4 min. DLLME was done using n-hexanol as an extracting solvent and ethanol as a dispersing solvent in a 1:8 v/v percent mixture. The Al-LMG complex was extracted by an organic solvent and separated from the aqueous phase within the syringe of an automated syringe pump. Two devices were specially developed for this work. These were (a) the fluorescence detector and accompanying flow cell for the organic phase enriched with the reaction product and (b) a heating device integrated into the holding coil to accelerate the slow reaction kinetics. The limits of detection (3σ) and quantification (10σ) were 8.0 ± 0.5 nmol L(-1) and 26.7 ± 1.6 nmol L(-1), respectively. The relative standard deviation for eight replicate determinations of 200 nmol L(-1) Al(3+) was <1.5%. The calibration graph using the preconcentration system was linear up to 1000 nmol L(-1) with a correlation coefficient of 0.999. Ambient concentrations of samples were quantifiable with found concentrations ranging from 43 to 142 nmol L(-1). Standard additions gave analyte recoveries from 97% to 113% proving the general applicability and adequateness of the analyzer system to real sample analysis.
Sensitive detection of trace aluminium in biological tissues by confocal laser scanning microscopy after staining with Lumogallion
Analyst 1998 Apr;123(4):759-62.PMID:9684408DOI:10.1039/a704876i.
This paper describes a method for the sensitive detection of aluminium in biological tissues by real time confocal laser scanning microscopy after staining with Lumogallion. The method enabled detection of aluminium > or = 9 micrograms g-1 in bone and is more sensitive than the conventional histochemical methods with aluminium and solochrome azurine, etc. Lumogallion reacts specifically with aluminium to form a fluorescent complex so that the proposed method is useful for detection and identification of aluminium in tissues.