Lycopene
(Synonyms: 番茄红素) 目录号 : GN10448
番茄红素（Lycopene）是一种脂溶性类胡萝卜素分子，在红色水果和蔬菜中含量很高，具有强大的抗氧化作用。
Cas No.:502-65-8
Sample solution is provided at 25 µL, 10mM.
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Lycopene is a fat-soluble carotenoid molecule that is found in high concentrations in red fruits and vegetables and has a strong antioxidant effect [1]. Lycopene can inhibit the activity of HMG-CoA reductase[2]. Lycopene can induce G0/G1 arrest and S phase arrest of the cell cycle[3].
In vitro, Lycopene (1-5μM) treatment of HT-29, T84, and MCF-7 cells for 48h significantly reduced the number of viable cells, with a 30% reduction in HT-29 and T84 cells and a 10% reduction in MCF-7 cells, and also caused cell cycle arrest at different stages [4]. Lycopene (0.5-10μM) treatment of SK-Hep-1 cells reduced the production of intracellular ROS by inhibiting the NADPH oxidase produced in the PKC pathway [5].
In vivo, oral treatment of mice inoculated with SK-Hep-1 tumor cells with Lycopene (20 mg/kg) for 12 weeks significantly reduced the average number of tumors and tumor cross-sectional area, and also reduced the positive rate of proliferating cell nuclear antigen (PCNA) in lung tissue, as well as the expression of PCNA, MMP-9 and VEGF proteins [6]. Oral treatment of diabetic rats with Lycopene (10 mg/kg) significantly reduced the activity of angiotensin-converting enzyme (ACE) in plasma and heart tissue [7].
References:
[1] Wang Q, Wu X, Zhang J, et al. Role of ROS/JAK2/STAT3 signaling pathway in di-n-butyl phthalate-induced testosterone synthesis inhibition and antagonism of lycopene[J]. Food and Chemical Toxicology, 2023, 175: 113741.
[2] Palozza P, Catalano A, Simone R E, et al. Effect of lycopene and tomato products on cholesterol metabolism[J]. Annals of Nutrition and Metabolism, 2012, 61(2): 126-134.
[3] Hwang E S, Bowen P E. Cell cycle arrest and induction of apoptosis by lycopene in LNCaP human prostate cancer cells[J]. Journal of medicinal food, 2004, 7(3): 284-289.
[4] Teodoro A J, Oliveira F L, Martins N B, et al. Effect of lycopene on cell viability and cell cycle progression in human cancer cell lines[J]. Cancer cell international, 2012, 12: 1-9.
[5] Bandeira A C B, da Silva T P, de Araujo G R, et al. Lycopene inhibits reactive oxygen species production in SK-Hep-1 cells and attenuates acetaminophen-induced liver injury in C57BL/6 mice[J]. Chemico-Biological Interactions, 2017, 263: 7-17.
[6] Huang C S, Liao J W, Hu M L. Lycopene inhibits experimental metastasis of human hepatoma SK-Hep-1 cells in athymic nude mice[J]. The Journal of nutrition, 2008, 138(3): 538-543.
[7] Ozmutlu S, Dede S, Ceylan E. The effect of lycopene treatment on ACE activity in rats with experimental diabetes[J]. Journal of the Renin-Angiotensin-Aldosterone System, 2012, 13(3): 328-333.
番茄红素（Lycopene）是一种脂溶性类胡萝卜素分子，在红色水果和蔬菜中含量很高，具有强大的抗氧化作用[1]。Lycopene能够抑制HMG-CoA还原酶的活性[2]。Lycopene可诱导细胞周期G0/G1期阻滞和S期阻滞[3]。
在体外，Lycopene（1-5μM）处理HT-29、T84、MCF-7细胞48h，显著减少了活细胞数量，HT-29和T84细胞减少了30%，MCF-7细胞减少了10%，还使细胞周期停滞在不同阶段[4]。Lycopene（0.5-10μM）处理SK-Hep-1细胞，通过抑制PKC途径中产生的NADPH氧化酶来降低细胞内ROS的产生[5]。
在体内，Lycopene（20mg/kg）通过口服治疗接种SK-Hep-1肿瘤细胞的小鼠12周，显著减少肿瘤平均数量和肿瘤横截面积，还降低了肺组织中增殖细胞核抗原（PCNA）的阳性率，以及PCNA、MMP-9和VEGF蛋白的表达[6]。Lycopene（10mg/kg）通过口服治疗糖尿病大鼠，显著降低了血浆和心脏组织中血管紧张素转化酶（ACE）的活性[7]。
| Cell experiment [1]: | |
Cell lines | HT-29、T84、MCF-7 cells |
Preparation Method | Three cancer cells lines (HT-29, T84 and MCF-7) were treated with lycopene (1–5 μM) for 48 and 96 h. Cell viability was monitored using the method of MTT. |
Reaction Conditions | 1-5μM; 48、96h |
Applications | After treatment for 48 h, a significant decrease in the number of viable cells was observed in three cancer cells lines as compared to controls, reaching 30% reduction in HT-29 and T84 cells and 10% in MCF-7 cells. |
| Animal experiment [2]: | |
Animal models | Adult male athymic nude mice |
Preparation Method | Mice were orally administered with lycopene (1 and 20 mg/kg) or β-carotene (20 mg/kg) in corn oil twice per week for 12 wk. Corn oil alone was given to the noninjected control mice. Two weeks into the supplementation, the mice were injected once with SK-Hep-1 cells into the lateral tail vein and blood samples were drawn once per week from the retro-orbital plexus for the measurement of MMP and VEGF. |
Dosage form | 1, 20mg/kg; p.o. |
Applications | High lycopene supplementation significantly reduced the mean number of tumors and the cross-sectional area of tumors. High lycopene supplementation also decreased the positive rate of proliferating cellular nuclear antigen (PCNA), and protein expressions of PCNA, MMP-9, and VEGF in lung tissues. |
References: [1] Teodoro A J, Oliveira F L, Martins N B, et al. Effect of lycopene on cell viability and cell cycle progression in human cancer cell lines[J]. Cancer cell international, 2012, 12: 1-9. [2]Huang C S, Liao J W, Hu M L. Lycopene inhibits experimental metastasis of human hepatoma SK-Hep-1 cells in athymic nude mice[J]. The Journal of nutrition, 2008, 138(3): 538-543. | |
| Cas No. | 502-65-8 | SDF | |
| 别名 | 番茄红素 | ||
| 化学名 | (6E,8E,10E,12E,14E,16E,18E,20E,22E,24E,26E)-2,6,10,14,19,23,27,31-octamethyldotriaconta-2,6,8,10,12,14,16,18,20,22,24,26,30-tridecaene | ||
| Canonical SMILES | CC(=CCCC(=CC=CC(=CC=CC(=CC=CC=C(C)C=CC=C(C)C=CC=C(C)CCC=C(C)C)C)C)C)C | ||
| 分子式 | C40H56 | 分子量 | 536.89 |
| 溶解度 | <3.03 mg/mL in 0.5MNAOH | 储存条件 | Store at -20°C, protect from light, stored under nitrogen |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 1.8626 mL | 9.3129 mL | 18.6258 mL |
| 5 mM | 0.3725 mL | 1.8626 mL | 3.7252 mL |
| 10 mM | 0.1863 mL | 0.9313 mL | 1.8626 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
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