m6A-ATP,100mM Sodium Solution
目录号 : GB20006
m6A-ATP,100mM Sodium Solution是一种N6修饰的ATP衍生物，作为腺苷激动剂，ED50值为17250nM。
Sample solution is provided at 25 µL, 10mM.
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m6A-ATP,100mM Sodium Solution is the N6-modified ATP derivative, acting as the adenosine agonist with an ED50 value of 17250nM[1]. m6A-ATP,100mM Sodium Solution can be used to synthesize oligopeptide chains in vitro and serves as a crucial substrate for in vitro oligopeptide synthesis. Both unmethylated and N6-methylated RNA sequences were generated through in vitro transcription, utilizing ATP and m6A-ATP as distinct nucleotide precursors, respectively[2]. m6A-ATP,100mM Sodium Solution can be used as raw materials to synthesize methylated mRNA and non-coding RNAs (ncRNAs), and m6A-modified RNA has increased stability and participates in translation and intracellular localization regulation[3]. m6A-ATP,100mM Sodium Solution can affect the ATP probe labeling efficiency of GSK3α. The ATP probe labeling efficiencies for GSK3α were reduced by 15%, 44%, and 64% at concentrations of 10, 100, and 200μM m6A-ATP, respectively, with concentration-dependent inhibition[4]. Incubation with 250μM m6A-ATP at physiological temperature (37°C) for 2 hours facilitated GST-tagged GSK3β-mediated phosphorylation of a glycogen synthase 1-derived peptide substrate (YRRAAVPPSPSLSRHSSPHQ(pS)EDEEE)[4].
References:
[1] Ribeiro J A, Sebastião A M. On the type of receptor involved in the inhibitory action of adenosine at the neuromuscular junction[J]. British journal of pharmacology, 1985, 84(4): 911.
[2] Xiao Y L, Liu S, Ge R, et al. Transcriptome-wide profiling and quantification of N 6-methyladenosine by enzyme-assisted adenosine deamination[J]. Nature biotechnology, 2023, 41(7): 993-1003.
[3]Sendinc E, Shi Y. RNA m6A methylation across the transcriptome[J]. Molecular cell, 2023, 83(3): 428-441.
[4] Dong X, Sun J, Miao W, et al. Proteome-Wide Characterizations of N 6-Methyl-Adenosine Triphosphate-and N 6-Furfuryl-Adenosine Triphosphate-Binding Capabilities of Kinases[J]. Analytical chemistry, 2021, 93(39): 13251-13259.
m6A-ATP,100mM Sodium Solution是一种N6修饰的ATP衍生物，作为腺苷激动剂，ED50值为17250nM[1]。m6A-ATP,100mM Sodium Solution可作为体外寡肽合成的重要底物，用于体外合成寡肽链。通过体外转录，分别以ATP和m6A-ATP作为不同的核苷酸前体，可生成未甲基化和N6甲基化的RNA序列[2]。m6A-ATP,100mM Sodium Solution可作为合成甲基化mRNA和非编码RNA（ncRNAs）的原料，m6A修饰的RNA稳定性增强，参与翻译过程和细胞内定位调控[3]。m6A-ATP,100mM Sodium Solution能够影响GSK3α的ATP探针标记效率，在10、100和200μM浓度的m6A-ATP处理后，GSK3α的ATP探针标记效率分别降低了15%、44%和64%，表现出浓度依赖性抑制作用[4]。当使用250μM m6A-ATP在生理温度（37°C）下孵育2小时，可促进GST标记-GSK3β介导的糖原合成酶1衍生肽底物（YRRAAVPPSPSLSRHSSPHQ(pS)EDEEE）的磷酸化反应[4]。
| Kinase experiment [1]: | |
Preparation Method | The kinase activity of GSK3β was evaluated using an in vitro kinase assay with a phosphopeptide substrate derived from glycogen synthase. The substrate peptide, YRRAAVPPSPSLSRHSSPHQ(pS)EDEEE, was designed to mimic the primed phosphorylation site of glycogen synthase. The assay was conducted by incubating 1.0μM of GST-tagged GSK3β with 10μM of the substrate peptide in the presence of 250μM ATP, m6A-ATP, or N6-furfuryl-ATP at 37°C for 2 hours. The reaction was carried out in a 100mM sodium solution, which was diluted to achieve a final concentration of 250μM for the nucleotide triphosphates. To assess the kinase activity, the phosphorylation states of the substrate peptide were analyzed using electrospray ionization mass spectrometry (ESI-MS) in "ultrazoom" scan mode. The [M + 3H]3+ ions corresponding to the mono-, di-, and tri-phosphorylated forms of the peptide were monitored to determine the relative abundances of each phosphorylated species. This approach allowed for the quantification of GSK3β activity by calculating the conversion ratios of the different phosphorylated forms of the peptide. |
Reaction Conditions | 250μM, 2h |
Applications | m6A-ATP facilitated the phosphorylation of 67.8% of the GSK3β substrate peptide. |
References: | |
| Cas No. | SDF | ||
| 分子式 | C11H18N5O13P3(free acid) | 分子量 | 521.21(free acid) |
| 溶解度 | 储存条件 | Store at -20°C | |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 1.9186 mL | 9.5931 mL | 19.1861 mL |
| 5 mM | 0.3837 mL | 1.9186 mL | 3.8372 mL |
| 10 mM | 0.1919 mL | 0.9593 mL | 1.9186 mL |
Quality Control & SDS
- View current batch:
- Purity: >99.50%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet