Malantide (trifluoroacetate salt)
目录号 : GC91851Malantide is a synthetic peptide derived from the β-subunit of human phosphorylase kinase and is a fluorescent substrate for PKA and PKC.
Sample solution is provided at 25 µL, 10mM.
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Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Malantide is a synthetic peptide derived from the β-subunit of human phosphorylase kinase and is a fluorescent substrate for PKA and PKC.[1],[2] Upon phosphorylation by PKA or PKC, the intrinsic tyrosine fluorescence decreases and can be used to quantify PKA and PKC activity. Malantide displays an emission at 302 nm upon excitation at 285 nm.
References:
[1].Malencik, D.A., and Anderson, S.R.Characterization of a fluorescent substrate for the adenosine 3',5'-cyclic monophosphate-dependent protein kinaseAnal. Biochem.132(1)34-40(1983).
[2].Zhao, Z.H., Malencik, D.A., and Anderson, S.R.Characterization of a new substrate for protein kinase C: Assay by continuous fluorometric monitoring and high performance liquid chromatographyBiochem. Biophys. Res. Commun.176(3)1454-1461(1991).
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 0.612 mL | 3.0602 mL | 6.1203 mL |
| 5 mM | 0.1224 mL | 0.612 mL | 1.2241 mL |
| 10 mM | 0.0612 mL | 0.306 mL | 0.612 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
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