Maoecrystal A
(Synonyms: 毛萼晶A) 目录号 : GC38980
Maoecrystal A 是可从I. eriocalyx 叶子中分离得到的天然化合物。
Cas No.:96850-30-5
Sample solution is provided at 25 µL, 10mM.
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Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Maoecrystal A is a compound isolated from leaves of Isodon eriocalyx[1].
[1]. Liang Xu, et al. Exhaustive degradation of the ring D of 3,20-epoxy ent-kaurane-type diterpene maoecrystal A. Tetrahedron 61 (2005) 4467-44.
| Cas No. | 96850-30-5 | SDF | |
| 别名 | 毛萼晶A | ||
| Canonical SMILES | CC(O[C@H](C1=C)[C@]2(C[C@@]1([H])CC3)[C@]3([H])[C@@](C(C4)=O)(CO[C@@]4([H])C5(C)C)[C@]5([H])[C@H](O)C2=O)=O | ||
| 分子式 | C22H28O6 | 分子量 | 388.45 |
| 溶解度 | DMSO : 33.33 mg/mL (85.80 mM; Need ultrasonic) | 储存条件 | Store at -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 2.5743 mL | 12.8717 mL | 25.7433 mL |
| 5 mM | 0.5149 mL | 2.5743 mL | 5.1487 mL |
| 10 mM | 0.2574 mL | 1.2872 mL | 2.5743 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Developing an UPLC-MS/MS method to quantify Maoecrystal A in rat plasma: Application to a pharmacokinetic study
J Chromatogr B Analyt Technol Biomed Life Sci 2018 Jun 1;1086:105-109.PMID:29660663DOI:10.1016/j.jchromb.2018.04.012.
Maoecrystal A (MC-A) is an ent-kaurane-type diterpene isolated from Rabdosia eriocalyx (Dunn) Hara. MC-A has been reported to show different types of pharmacological activities, including anticancer, anti-inflammatory and bacteriostatic functions. However, bioanalysis of MC-A has not been reported. The purpose of this study is to develop an UPLC-MS/MS method to quantify MC-A in plasma and determine its pharmacokinetic properties using an animal model. The separation was performed using a Waters HSS T3 column (50 mm × 2.1 mm, 1.8 μm, Waters Corp., Milford, MA, USA) with methanol and water containing 0.1% of formic acid as the mobile phases. The mass analysis was performed in a Waters Xevo TQ mass spectrometer using multiple reaction monitoring (MRM) in positive scan mode. Protein precipitation was used to extract the drug from rat plasma samples. The calibration curve is linear in the concentration range 0.49-2000.0 ng/mL. The extraction recovery and the matrix effect were 78.11 to 91.72% and 90.38 to 98.02%, respectively. The RSD of inter/intra-day precisions were <13.72% and the accuracy was >86.41%. Stability studies showed that MC-A was stable (RSD < 14.98%) at different conditions (i.e., short-term, long-term, bench, and three freeze-thaw cycles) in rat plasma. The method was successfully applied to a pharmacokinetic study using rats through oral and intravenous administration routes. The oral bioavailability of MC-A was only 2.9%. Further studies are needed to determine the absorption and metabolism in order to improve the oral bioavailability of MC-A.
Novel rearrangement products of the degradated derivative of Maoecrystal A, an ent-kaurane-type diterpene
J Asian Nat Prod Res 2007 Apr-Aug;9(3-5):239-43.PMID:17566916DOI:10.1080/10286020600603981.
Two novel rearrangement products of the degradated derivative of Maoecrystal A (1), an ent-kaurane-type diterpene via a Wagner-Meerwein process, have been reported.
Cytotoxic 3,20-epoxy-ent-kaurane diterpenoids from Isodon eriocalyx var. laxiflora
J Nat Prod 2002 Dec;65(12):1892-6.PMID:12502334DOI:10.1021/np020059e.
Four new ent-kaurane diterpenoids, laxiflorins J-M (1-4), along with Maoecrystal A (5) and maoecrystal P (6), were isolated from the leaves of Isodon eriocalyx var. laxiflora. Their structures were determined by spectroscopic analyses. All the compounds were assayed for their cytotoxic effects on human tumor K562, A549, and T24 cell lines. Compounds 1 and 6 showed significant inhibitory effects on human tumor K562 and T24 cells, with IC(50) values less than 0.5 microg/mL. Compound 3 also demonstrated cytotoxic activities against all three types of human tumor cells, with IC(50) values in the range of 1-25 microg/mL.