MDL 72527 dihydrochloride
(Synonyms: N1,N4-二(BUTA-2,3-二ENYL)丁烷-1,4-二胺双盐酸盐) 目录号 : GC10545A PAO inhibitor
Cas No.:93565-01-6
Sample solution is provided at 25 µL, 10mM.
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Quality Control & SDS
- View current batch:
- Purity: >97.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
MDL 72527 is a polyamine oxidase (PAO) inhibitor that inhibits spermine oxidase and N1-acetylpolyamine oxidase (IC50s = 6.1 and 0.02 µM, respectively).[1] It does not inhibit monoamine oxidase or D-amino acid oxidase. MDL 72527 inhibits PAO-dependent H2O2 production in vitro and in vivo and is used to study the role of PAO activity in diverse models, including infection, cancer, and hyperoxia.[2][3][4]
Reference:
[1]. Moriya, S.S., Miura, T., Takao, K., et al. Development of irreversible inactivators of spermine oxidase and N1-acetylpolyamine oxidase. Biol. Pharm. Bull. 37(3), 475-480 (2014).
[2]. Chaturvedi, R., Cheng, Y., Asim, M., et al. Induction of polyamine oxidase 1 by Helicobacter pylori causes macrophage apoptosis by hydrogen peroxide release and mitochondrial membrane depolarization. J. Biol. Chem. 279(38), 40161-40173 (2004).
[3]. Goodwin, A.C., Destefano, C.E., Wu, S., et al. Polyamine catabolism contributes to enterotoxigenic Bacteroides fragilis-induced colon tumorigenesis. Proc. Natl. Acad. Sci. USA 108(37), 15354-15359 (2011).
[4]. Patel, C.A., Xu, Z., Xing, J., et al. Treatment with polyamine oxidase inhibitor reduces microglial activation and limits vascular injury in ischemic retinopathy. Biochim Biophys. Acta. 1862(9), 1628-1639 (2016).
| 制备储备液 | |||
 |
1 mg | 5 mg | 10 mg |
| 1 mM | 3.7703 mL | 18.8516 mL | 37.7031 mL |
| 5 mM | 0.7541 mL | 3.7703 mL | 7.5406 mL |
| 10 mM | 0.377 mL | 1.8852 mL | 3.7703 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。