Medroxyprogesterone acetate
(Synonyms: 醋酸甲羟孕酮; Medroxyprogesterone 17-acetate; Farlutin) 目录号 : GC12974
A synthetic progestogen
Cas No.:71-58-9
Sample solution is provided at 25 µL, 10mM.
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Medroxyprogesterone acetate is a widely used synthetic steroid by its interaction with progesterone, androgen and glucocorticoid receptors[1]。
References:
[1]. Schindler, A.E., et al., Classification and pharmacology of progestins. Maturitas, 2008. 61(1-2): p. 171-80.
[2]. Lenco, W., M. McKnight, and A.S. Macdonald, Effects of cortisone acetate, methylprednisolone and medroxyprogesterone on wound contracture and epithelization in rabbits. Ann Surg, 1975. 181(1): p. 67-73.
| Cell experiment [1]: | |
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Cell lines |
M-1 cells |
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Preparation method |
The solubility of this compound in DMSO is > 10 mM. General tips for obtaining a higher concentration: Please warm the tube at 37 °C for 10 minutes and/or shake it in the ultrasonic bath for a while. Stock solution can be stored below - 20 °C for several months. |
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Reacting condition |
1 nM ~ 1 μM; 24 hrs |
|
Applications |
In M-1 cells, Medroxyprogesterone Acetate at the dose of 1 μM increased α-epithelial Na channel (α-ENaC) and serum and glucocorticoid-regulated kinase 1 (sgk1) expression. Medroxyprogesterone Acetate dose-dependently increased α-ENaC expression with the earliest effect seen at 10 nM. In Medroxyprogesterone Acetate-treated M-1 cells, α-ENaC-driven luciferase activity could not be inhibited by Org31710, which indicated that Medroxyprogesterone Acetate regulated α-ENaC in a progesterone receptor (PR)-independent manner. |
| Animal experiment [2]: | |
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Animal models |
Rats |
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Dosage form |
14 and 21 mg/2 mL; given via osmotic pumps |
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Applications |
In aged ovariectomized rats, Medroxyprogesterone acetate impaired delayed memory retention on the water radial-arm maze, and exacerbated overnight forgetting on the Morris maze. Medroxyprogesterone acetate significantly decreased the level of glutamic acid decarboxylase (GAD) in the hippocampus, and increased GAD level in the entorhinal cortex. |
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Other notes |
Please test the solubility of all compounds indoor, and the actual solubility may slightly differ with the theoretical value. This is caused by an experimental system error and it is normal. |
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References: [1]. Thomas CP, Liu KZ, Vats HS. Medroxyprogesterone acetate binds the glucocorticoid receptor to stimulate alpha-ENaC and sgk1 expression in renal collecting duct epithelia. Am J Physiol Renal Physiol. 2006 Feb;290(2):F306-12. Epub 2005 Sep 27. [2]. Braden BB, Talboom JS, Crain ID, Simard AR, Lukas RJ, Prokai L, Scheldrup MR, Bowman BL, Bimonte-Nelson HA. Medroxyprogesterone acetate impairs memory and alters the GABAergic system in aged surgically menopausal rats. Neurobiol Learn Mem. 2010 Mar;93(3):444-53. | |
| Cas No. | 71-58-9 | SDF | |
| 别名 | 醋酸甲羟孕酮; Medroxyprogesterone 17-acetate; Farlutin | ||
| 化学名 | [(6S,8R,9S,10R,13S,14S,17R)-17-acetyl-6,10,13-trimethyl-3-oxo-2,6,7,8,9,11,12,14,15,16-decahydro-1H-cyclopenta[a]phenanthren-17-yl] acetate | ||
| Canonical SMILES | CC1CC2C(CCC3(C2CCC3(C(=O)C)OC(=O)C)C)C4(C1=CC(=O)CC4)C | ||
| 分子式 | C24H34O4 | 分子量 | 386.52 |
| 溶解度 | ≥ 9.5mg/mL in DMSO with gentle warming | 储存条件 | Store at 4°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
 |
1 mg | 5 mg | 10 mg |
| 1 mM | 2.5872 mL | 12.9359 mL | 25.8719 mL |
| 5 mM | 0.5174 mL | 2.5872 mL | 5.1744 mL |
| 10 mM | 0.2587 mL | 1.2936 mL | 2.5872 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Quality Control & SDS
- View current batch:
- Purity: >99.50%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet