MEGA-10
(Synonyms: N-decanoyl-N-Methylglucamine) 目录号 : GC44151A detergent used to solubilize membrane proteins
Cas No.:85261-20-7
Sample solution is provided at 25 µL, 10mM.
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MEGA-10 is a nonionic detergent that can be used to solubilize membrane proteins. [1] It has a critical micelle concentration (CMC) of 4.88 mM under no salt conditions, which decreases in the presence of sodium chloride. [2] MEGA-10 has been used to reconstitute amino acid transporters from rat liver plasma membrane vesicles into artificial phospholipid membranes.[3] It has also been used to solubilize the melibiose transport carrier from E. coli membranes and reconstitute it into liposomes.[4]
Reference:
[1]. Hildreth, J.E.K. N-D-Gluco-N-methylalkanamide compounds, a new class of non-ionic detergents for membrane biochemistry. Biochem. J. 207(2), 363-366 (1982).
[2]. Molina-Bolívar, J.A., Hierrezuelo, J.M., and Carnero Ruiz, C. Self-assembly, hydration, and structures in N-decanoyl-N-methylglucamide aqueous solutions: Effect of salt addition and temperature. J. Colloid. Interface Sci. 313(2), 656-664 (2007).
[3]. Quesada, A.R., and McGivan, J.D. A rapid method for the functional reconstitution of amino acid transport systems from rat liver plasma membranes. Partial purification of System A. Biochem J. 255(3), 963-969 (1988).
[4]. Hanatani, M., Nishifuji, K., Futai, M., et al. Solubilization and reconstitution of membrane proteins of Escherichia coli using alkanoyl-N-methylglucamides. J. Biochem. 95(5), 1349-1353 (1984).
| Cas No. | 85261-20-7 | SDF | |
| 别名 | N-decanoyl-N-Methylglucamine | ||
| 化学名 | 1-deoxy-1-[methyl(1-oxodecyl)amino]-D-glucitol | ||
| Canonical SMILES | OC[C@@H](O)[C@@H](O)[C@H](O)[C@@H](O)CN(C)C(CCCCCCCCC)=O | ||
| 分子式 | C17H35NO6 | 分子量 | 349.5 |
| 溶解度 | 1mg/mL in ethanol, 30mg/mL in DMSO or in DMF | 储存条件 | Store at -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
 |
1 mg | 5 mg | 10 mg |
| 1 mM | 2.8612 mL | 14.3062 mL | 28.6123 mL |
| 5 mM | 0.5722 mL | 2.8612 mL | 5.7225 mL |
| 10 mM | 0.2861 mL | 1.4306 mL | 2.8612 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Quality Control & SDS
- View current batch:
- Purity: >99.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet