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MEGA-10

(Synonyms: N-decanoyl-N-Methylglucamine) 目录号 : GC44151

A detergent used to solubilize membrane proteins

MEGA-10 Chemical Structure

Cas No.:85261-20-7

规格 价格 库存 购买数量
500mg
¥619.00
现货
1g
¥1,095.00
现货
5g
¥4,521.00
现货

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Sample solution is provided at 25 µL, 10mM.

Description

MEGA-10 is a nonionic detergent that can be used to solubilize membrane proteins. [1] It has a critical micelle concentration (CMC) of 4.88 mM under no salt conditions, which decreases in the presence of sodium chloride. [2] MEGA-10 has been used to reconstitute amino acid transporters from rat liver plasma membrane vesicles into artificial phospholipid membranes.[3] It has also been used to solubilize the melibiose transport carrier from E. coli membranes and reconstitute it into liposomes.[4]

Reference:
[1]. Hildreth, J.E.K. N-D-Gluco-N-methylalkanamide compounds, a new class of non-ionic detergents for membrane biochemistry. Biochem. J. 207(2), 363-366 (1982).
[2]. Molina-Bolívar, J.A., Hierrezuelo, J.M., and Carnero Ruiz, C. Self-assembly, hydration, and structures in N-decanoyl-N-methylglucamide aqueous solutions: Effect of salt addition and temperature. J. Colloid. Interface Sci. 313(2), 656-664 (2007).
[3]. Quesada, A.R., and McGivan, J.D. A rapid method for the functional reconstitution of amino acid transport systems from rat liver plasma membranes. Partial purification of System A. Biochem J. 255(3), 963-969 (1988).
[4]. Hanatani, M., Nishifuji, K., Futai, M., et al. Solubilization and reconstitution of membrane proteins of Escherichia coli using alkanoyl-N-methylglucamides. J. Biochem. 95(5), 1349-1353 (1984).

化学性质

Cas No. 85261-20-7 SDF
别名 N-decanoyl-N-Methylglucamine
化学名 1-deoxy-1-[methyl(1-oxodecyl)amino]-D-glucitol
Canonical SMILES OC[C@@H](O)[C@@H](O)[C@H](O)[C@@H](O)CN(C)C(CCCCCCCCC)=O
分子式 C17H35NO6 分子量 349.5
溶解度 1mg/mL in ethanol, 30mg/mL in DMSO or in DMF 储存条件 Store at -20°C
General tips 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效。
储备液的保存方式和期限:-80°C 储存时,请在 6 个月内使用,-20°C 储存时,请在 1 个月内使用。
为了提高溶解度,请将管子加热至37℃,然后在超声波浴中震荡一段时间。
Shipping Condition 评估样品解决方案:配备蓝冰进行发货。所有其他可用尺寸:配备RT,或根据请求配备蓝冰。

溶解性数据

制备储备液
1 mg 5 mg 10 mg
1 mM 2.8612 mL 14.3062 mL 28.6123 mL
5 mM 0.5722 mL 2.8612 mL 5.7225 mL
10 mM 0.2861 mL 1.4306 mL 2.8612 mL
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