Metformin D6 hydrochloride
(Synonyms: 二甲双胍盐酸,1,1-Dimethylbiguanide-d6 hydrochloride) 目录号 : GC61045An internal standard for the quantification of metformin
Cas No.:1185166-01-1
Sample solution is provided at 25 µL, 10mM.
Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Metformin-d6 is intended for use as an internal standard for the quantification of metformin by GC- or LC-MS. Metformin is a biguanide with diverse biological activities.1,2,3,4 Metformin (250 mg/kg, i.p.) increases hepatic AMPK activity and reduces blood glucose by more than 50% in a liver kinase B1-dependent manner in mice fed normal and high-fat diets, respectively, and reduces blood glucose by 40% in ob/ob mice.2 It reduces weight gain, hepatic lipid droplet content, and total cholesterol, LDL cholesterol, and triglyceride levels in the plasma of diet-induced obese mice when administered at doses of 10 or 50 mg/kg per day.4 It also reverses increased hepatic triglyceride and apolipoprotein A5 levels, as well as hepatic steatosis, in a dose-dependent manner in an ob/ob mouse model of non-alcoholic fatty liver disease (NAFLD).5 Metformin (250 mg/kg) reduces tumor growth in an HCT116 p53-\- human colon cancer mouse xenograft model, but has no effect on HCT116 p53-\- tumors overexpressing recombinant S. cerevisiae Ndi1 NADH dehydrogenase, a single-subunit ortholog of the multi-subunit mammalian mitochondrial complex I.3 Formulations containing metformin have been used in the treatment of type 2 diabetes.
1.Viollet, B., Guigas, B., Garcia, N.S., et al.Cellular and molecular mechanisms of metformin: An overviewClin. Sci. (Lond.)122(6)253-270(2012) 2.Shaw, R.J., Lamia, K.A., Vasquez, D., et al.The kinase LKB1 mediates glucose homeostasis in liver and therapeutic effects of metforminScience3101642-1646(2005) 3.Wheaton, W.W., Weinberg, S.E., Hamanaka, R.B., et al.Metformin inhibits mitochondrial complex I of cancer cells to reduce tumorigenesisElife3:e02242(2014) 4.Kim, E.K., Lee, S.H., Jhun, J.Y., et al.Metformin prevents fatty liver and improves balance of white/brown adipose in an obesity mouse model by inducing FGF21.Mediators Inflamm.5813030(2016) 5.Lin, M.-J., Dai, W., Scott, M.J., et al.Metformin improves nonalcoholic fatty liver disease in obese mice via down-regulation of apolipoprotein A5 as part of the AMPK/LXRα signaling pathwayOncotarget8\(65)108802-108809(2017)
Cas No. | 1185166-01-1 | SDF | |
别名 | 二甲双胍盐酸,1,1-Dimethylbiguanide-d6 hydrochloride | ||
Canonical SMILES | NC(NC(N(C([2H])([2H])[2H])C([2H])([2H])[2H])=N)=N.[H]Cl | ||
分子式 | C4H6D6ClN5 | 分子量 | 171.66 |
溶解度 | H2O : 50 mg/mL (291.27 mM; Need ultrasonic); DMSO : 50 mg/mL (291.27 mM; ultrasonic and warming and heat to 60°C) | 储存条件 | Store at -20°C |
General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效。 储备液的保存方式和期限:-80°C 储存时,请在 6 个月内使用,-20°C 储存时,请在 1 个月内使用。 为了提高溶解度,请将管子加热至37℃,然后在超声波浴中震荡一段时间。 |
||
Shipping Condition | 评估样品解决方案:配备蓝冰进行发货。所有其他可用尺寸:配备RT,或根据请求配备蓝冰。 |
制备储备液 | |||
1 mg | 5 mg | 10 mg | |
1 mM | 5.8255 mL | 29.1273 mL | 58.2547 mL |
5 mM | 1.1651 mL | 5.8255 mL | 11.6509 mL |
10 mM | 0.5825 mL | 2.9127 mL | 5.8255 mL |
第一步:请输入基本实验信息(考虑到实验过程中的损耗,建议多配一只动物的药量) | ||||||||||
给药剂量 | mg/kg | 动物平均体重 | g | 每只动物给药体积 | ul | 动物数量 | 只 | |||
第二步:请输入动物体内配方组成(配方适用于不溶于水的药物;不同批次药物配方比例不同,请联系GLPBIO为您提供正确的澄清溶液配方) | ||||||||||
% DMSO % % Tween 80 % saline | ||||||||||
计算重置 |
计算结果:
工作液浓度: mg/ml;
DMSO母液配制方法: mg 药物溶于 μL DMSO溶液(母液浓度 mg/mL,
体内配方配制方法:取 μL DMSO母液,加入 μL PEG300,混匀澄清后加入μL Tween 80,混匀澄清后加入 μL saline,混匀澄清。
1. 首先保证母液是澄清的;
2.
一定要按照顺序依次将溶剂加入,进行下一步操作之前必须保证上一步操作得到的是澄清的溶液,可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。