Methoprene (ZR-515)
(Synonyms: 烯虫酯; ZR-515) 目录号 : GC32102
Methoprene (ZR-515)是一种生长调节杀虫剂，属于保幼激素类似物（JHA），通过模拟昆虫保幼激素的作用干扰其正常发育周期，从而抑制害虫种群增长。
Cas No.:40596-69-8
Sample solution is provided at 25 µL, 10mM.
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Methoprene (ZR-515) is a growth regulating insecticide belonging to the juvenile hormone analog (JHA). It inhibits the growth of pest populations by interfering with the normal development cycle of insects by simulating the effects of juvenile hormone[1]. Methoprene is widely used to control mosquito larvae (such as Aedes and Culex), blocking their development into adults, thereby reducing the spread of mosquito-borne diseases such as dengue fever and Zika virus[2]. Methoprene can interfere with midgut remodeling during mosquito metamorphosis[3]. Methoprene has extremely low toxicity to non-target organisms (such as mammals and birds), has excellent environmental safety, and is suitable for integrated pest management (IPM) systems[4].
In vitro, treatment of Spodoptera frugiperda Sf9 cell line with Methoprene (0-200μM) for 24-72h inhibited cell proliferation in a dose- and time-dependent manner and significantly induced cell G2/M phase arrest[5].
In vivo, Methoprene (2, 50, and 200μg/L) treatment of cherry shrimp Neocaridina Davidi reduced body length and molting frequency, and disrupted cuticle development and carbohydrate, peptide, and lipid metabolism[6].
References:
[1] Ramaseshadri P, Farkaš R, Palli S R. Recent progress in juvenile hormone analogs (JHA) research[J]. Advances in insect physiology, 2012, 43: 353-436.
[2] Cuervo-Parra J A, Cortés T R, Ramirez-Lepe M. Mosquito-borne diseases, pesticides used for mosquito control, and development of resistance to insecticides[J]. Insecticides resistance. Rijeka: InTechOpen, 2016, 2: 111-34.
[3] Nishiura J T, Ho P, Ray K. Methoprene interferes with mosquito midgut remodeling during metamorphosis[J]. Journal of medical entomology, 2003, 40(4): 498-507.
[4] Munjal K, Gauttam V K, Mir S R, et al. Insect Growth Regulators[M]//Biopesticides Handbook. CRC Press, 2023: 223-242.
[5] Giraudo M, Califano J, Hilliou F, et al. Effects of hormone agonists on Sf9 cells, proliferation and cell cycle arrest[J]. PLoS One, 2011, 6(10): e25708.
[6] Hu X L, Niu J J, Meng Q, et al. Effects of two juvenile hormone analogue insecticides, fenoxycarb and methoprene, on Neocaridina davidi[J]. Environmental Pollution, 2019, 253: 89-99.
Methoprene (ZR-515)是一种生长调节杀虫剂，属于保幼激素类似物（JHA），通过模拟昆虫保幼激素的作用干扰其正常发育周期，从而抑制害虫种群增长[1]。Methoprene广泛用于控制蚊虫幼虫（如伊蚊和库蚊），阻断其发育为成虫，从而减少登革热、寨卡病毒等蚊媒疾病的传播[2]。Methoprene能够干扰蚊子变态过程中的中肠重塑[3]。Methoprene对非靶标生物（如哺乳动物和鸟类）毒性极低，具有优异的环境安全性，适用于综合害虫管理（IPM）体系[4]。
在体外，Methoprene（0-200μM）处理Spodoptera frugiperda Sf9细胞系24-72h，以剂量和时间依赖性方式抑制了细胞增殖，显著诱导了细胞G2/M期阻滞[5]。
在体内，Methoprene（2, 50, 200μg/L）处理樱桃虾Neocaridina Davidi，降低了身体长度和换毛频率，干扰了角质层发育和碳水化合物，肽和脂质代谢[6]。
| Cell experiment [1]: | |
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Cell lines |
Spodoptera frugiperda Sf9 cell line |
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Preparation Method |
Sf9 cells were treated with 0-200μM Methoprene for 24, 48, and 72h, cell viability was measured by MTT assay. |
|
Reaction Conditions |
0-200μM; 24, 48, 72h |
|
Applications |
Methoprene inhibited cell proliferation in a dose- and time-dependent manner. |
|
References: |
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| Cas No. | 40596-69-8 | SDF | |
| 别名 | 烯虫酯; ZR-515 | ||
| Canonical SMILES | CC(C)(OC)CCCC(C)C/C=C/C(C)=C/C(OC(C)C)=O | ||
| 分子式 | C19H34O3 | 分子量 | 310.47 |
| 溶解度 | DMSO : ≥ 60 mg/mL (193.26 mM) | 储存条件 | Store at -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 3.2209 mL | 16.1046 mL | 32.2092 mL |
| 5 mM | 0.6442 mL | 3.2209 mL | 6.4418 mL |
| 10 mM | 0.3221 mL | 1.6105 mL | 3.2209 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet