Methyl-β-cyclodextrin (Methyl-beta-cyclodextrin)
(Synonyms: 甲基-β-环糊精; Methyl-beta-cyclodextrin) 目录号 : GC32697
A randomly methylated form of β-cyclodextrin
Cas No.:128446-36-6
Sample solution is provided at 25 µL, 10mM.
Methyl-β-cyclodextrin is a randomly methylated form of the cyclic oligosaccharide β-cyclodextrin .1 Methyl-β-cyclodextrin contains seven D-(+)-glucopyranose units that contain randomized hydrogen or methyl groups. Methyl-β-cyclodextrin has been used to improve the aqueous solubility of various compounds and to extract cholesterol from lipid membranes.1,2 Methyl-β-cyclodextrin (5 mM) reduces α-synuclein levels in the membrane and detergent-insoluble fractions from B103 neuroblastoma cells transfected with human α-synuclein.3 It also reduces α-synuclein levels in mouse brain in a transgenic model of α-synucleinopathy.
甲基-β-环糊精是环糊精β的随机甲基化形式。1甲基-β-环糊精含有七个D-(+)-葡萄糖吡喃糖单元，其中包含随机的氢或甲基基团。甲基-β-环糊精已被用于改善各种化合物的水溶性，并从脂质膜中提取胆固醇。1,2甲基-β-环糊精（5 mM）可以降低人类α-突触核蛋白在B103神经母细胞转染体的膜和洗涤剂不溶性部分中的水平。3它还可以降低转基因α-突触核蛋白病模型小鼠大脑中α-突触核蛋白的水平。
1.Katageri, A.R., and Sheikh, M.A.Cyclodextrin a gift to pharmaceutical world reviewInt. Res. J. Pharm.3(1)52-56(2012) 2.Besenicar, M.P., Bavdek, A., Kladnik, A., et al.Kinetics of cholesterol extraction from lipid membranes by methyl-β-cyclodextrin--a surface plasmon resonance approachBiochim. Biophys. Acta1778(1)175-184(2008) 3.Bar-On, P., Rockenstein, E., Adame, A., et al.Effects of the cholesterol-lowering compound methyl-β-cyclodextrin in models of α-synucleinopathyJ. Neurochem.98(4)1032-1045(2006)
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Cell experiment: |
PEL cells are incubated in triplicate in a 96-well microculture plate in the presence of different concentrations of methyl-β-cyclodextrin (0-10 mM) in a final volume of 0.1 mL for 24 h at 37°C. Subsequently, MTT (0.5 mg/mL final concentration) is added to each well. After 3 h of additional incubation, 100 μL of a 0.04 N HCl is added to dissolve the crystals. Absorption values at 570 nm are determined[1]. |
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Animal experiment: |
Mice: Female NRJ mice are intraperitoneally inoculated with BCBL-1 cells suspended in PBS. The mice are then treated with intraperitoneal injections of PBS or methyl-β-cyclodextrin (500 mg/kg per day). Tumor burdens are evaluated by measuring body weights and ascites[1]. |
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References: [1]. Mundhara N, et al. Methyl-β-cyclodextrin, an actin depolymerizer augments the antiproliferative potential of microtubule-targeting agents. Sci Rep. 2019 May 21;9(1):7638. |
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| Cas No. | 128446-36-6 | SDF | |
| 别名 | 甲基-β-环糊精; Methyl-beta-cyclodextrin | ||
| Canonical SMILES | CO[C@@H]1[C@H](O[R])[C@H](O[R])[C@@H](C)C(CO[R])O1.[7].[R=H or -CH3] | ||
| 分子式 | 分子量 | 1310(Average) | |
| 溶解度 | DMSO : ≥ 100 mg/mL;Water : ≥ 50 mg/mL | 储存条件 | Store at 4°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 0.7634 mL | 3.8168 mL | 7.6336 mL |
| 5 mM | 0.1527 mL | 0.7634 mL | 1.5267 mL |
| 10 mM | 0.0763 mL | 0.3817 mL | 0.7634 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
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