Methyl Blue
(Synonyms: 甲基蓝) 目录号 : GC39268
Methyl blue 属于三氨基三苯甲烷染料。Methyl blue 在多色染色法中被广泛用作抗菌染料，并在组织学和微生物学染色溶液中得到应用。Methyl blue 已经用作模型来研究各种催化剂对染料光降解的影响。
Cas No.:28983-56-4
Sample solution is provided at 25 µL, 10mM.
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Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Methyl blue belongs to the group of triaminotriphenylmethane dyes. Methyl blue is widely used as antiseptic dye in polychrome staining method and has applications in histological and microbiological staining solutions. Methyl blue has been used as a model to study the effect of various catalysts on photodegradation of dyes[1][2].
[1]. Mohammed Hassan, et al. Micellar effect on the kinetics of oxidation of methyl blue by Ce(IV) in sulfuric acid medium. Arabian Journal of Chemistry. Volume 8, Issue 1, January 2015, Pages 72-77. [2]. K.Kotak, et al. Photostability of Aniline Blue (CI 42755) and Methyl Blue (CI 42780). Dyes and Pigments. Volume 34, Issue 2, June 1997, Pages 159-167.
| Cas No. | 28983-56-4 | SDF | |
| 别名 | 甲基蓝 | ||
| Canonical SMILES | O=S(C1=CC=CC=C1/N=C2C=C/C(C=C\2)=C(C3=CC=C(NC4=CC=CC=C4S(=O)(O)=O)C=C3)\C5=CC=C(NC6=CC=CC=C6S(=O)([O-])=O)C=C5)([O-])=O.[Na+].[Na+] | ||
| 分子式 | C37H27N3Na2O9S3 | 分子量 | 799.8 |
| 溶解度 | H2O : 20 mg/mL (25.01 mM; Need ultrasonic) | 储存条件 | Store at -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 1.2503 mL | 6.2516 mL | 12.5031 mL |
| 5 mM | 0.2501 mL | 1.2503 mL | 2.5006 mL |
| 10 mM | 0.125 mL | 0.6252 mL | 1.2503 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Efficient adsorption of methyl orange and Methyl Blue dyes by a novel triptycene-based hyper-crosslinked porous polymer
RSC Adv 2022 Feb 16;12(9):5587-5594.PMID:35425553DOI:10.1039/d1ra08589a.
It is still a great challenge to develop new materials for the highly efficient entrapment of organic dyes from aqueous solution. Herein, a novel triptycene-based hyper-crosslinked porous polymer (TPP-PP) was designed and synthesized by a simple Friedel-Crafts reaction. The obtained polymer TPP-PP has a high surface area, abundant pore structure and stable thermal performance. Due to the above characteristics, TPP-PP has good adsorption performance for anionic methyl orange solution (MO) and cationic Methyl Blue solution (MB). Under the optimal experiment conditions, the TPP-PP showed an excellent adsorption capacity for MO (220.82 mg g-1) and MB (159.80 mg g-1), respectively. The adsorption kinetics fitted the pseudo-second-order model. The adsorption of MO by TPP-PP reaches equilibrium within 180 minutes, and the adsorption of MB reaches equilibrium within 150 minutes. The adsorption behavior was not only spontaneous but also endothermic in reality. At the same time, TPP-PP also has good reusability. After 5 cycles of experiments, the removal rate of MO and MB by TPP-PP can still reach more than 80%. Thus, the Friedel-Crafts reaction crosslinked method might be a promising approach for the synthesis of novel material for the highly efficient extraction of dye wastewater.
Methyl Blue and aniline blue versus patent blue and trypan blue as vital dyes in cataract surgery: capsule staining properties and cytotoxicity to human cultured corneal endothelial cells
J Cataract Refract Surg 2011 Jun;37(6):1147-53.PMID:21596258DOI:10.1016/j.jcrs.2010.12.051.
Purpose: To evaluate capsule-staining properties and biocompatibility of the triarylmethane dyes Methyl Blue and aniline blue compared with patent blue and trypan blue on cultured human corneal endothelial cells. Setting: Centre for Ophthalmology, University of Tuebingen, Tuebingen, Germany. Design: Experimental study. Methods: Human corneal endothelial cell cultures were harvested from human donor cells and exposed to various concentrations (0.025 to 5.0 mg/mL) of Methyl Blue, aniline blue, patent blue, and trypan blue. Cytotoxicity was assessed by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide test after 24 hours of incubation. Calcein live cell staining was performed at the same time point. The dyes were also used to stain pig lens capsules in vitro by incubating the lenses for 1 minute with 3 concentrations (0.5, 1.5, and 2.5 mg/mL) of dye, after which the staining properties were evaluated. Results: No significant cytotoxicity was detected for patent blue and Methyl Blue at any tested concentration. However, aniline blue exerted significant cytotoxicity at concentrations of 1.5 mg/mL or higher and trypan blue at 2.5 mg/mL or higher. Capsule staining of the tested triarylmethane dyes was suitable for performing capsulorhexis, but only at higher concentrations than with trypan blue. Conclusions: High concentrations and long incubation times of trypan blue and aniline blue showed significant cytotoxicity to human cultured endothelial cells in contrast to patent blue and Methyl Blue. All tested dyes were able to stain lens capsules sufficiently for capsulorhexis creation. Financial disclosure: No author has a financial or proprietary interest in any material or method mentioned.
KIT-6 induced mesostructured TiO2 for photocatalytic degradation of Methyl Blue
Environ Sci Pollut Res Int 2021 Oct;28(38):53340-53352.PMID:34031833DOI:10.1007/s11356-021-14442-z.
In this study, titania/silica nanocomposite and mesoporous TiO2 (m-TiO2) photocatalysts are developed by KIT-6 template via a sol-gel approach. The synthesized photocatalysts are characterized by XRD, EDX, SEM, Raman, PL, and UV-vis DRS analysis techniques. The as-synthesized series revealed a high surface area, smaller size, a greater number of accessible active sites, and enhanced light-harvesting capability. The m-TiO2 photocatalysts' charge recombination capability was curiously inferior to the rest of as-synthesized TiO2/KIT-6 nanocomposite materials. The band-gap of as-synthesized materials were suitable for their activity in UV light irradiations. It was pragmatic that the photocatalytic degradation efficiency of m-TiO2 photocatalysts was superior as compared to that of commercial TiO2 photocatalyst under UV light irradiations, owing to the synergistic outcome of the anatase phase and a greater number of accessible active-sites availability as a result of high surface area. Moreover, the m-TiO2 was critically evaluated by investigating various parameters affecting the photocatalytic degradation reaction of MB including the effect of irradiation time, pH, catalyst dosage, and dye concentration. The m-TiO2, 45wt% composite material and commercial-TiO2 exhibited 99.27, 91.20, and 84.67% degradation of Methyl Blue in 50 min, respectively. Finally, the m-TiO2 exhibited excellent recyclability with negligible loss of activity performance.
Oxide modified aluminum for removal of methyl orange and Methyl Blue in aqueous solution
RSC Adv 2021 Jan 4;11(2):867-875.PMID:35423697DOI:10.1039/d0ra09048d.
In this work, pristine aluminum (Al) powder was soaked in deionized water for a time period and then it was dried and heat-treated at 400 °C such that a layer of fine Al2O3 grains covered the Al particle surfaces, forming oxide modified Al powder (OM-Al). It was found that OM-Al greatly enhanced the efficiency in removing methyl orange (M-orange) and Methyl Blue (M-blue) in aqueous solution. The time to completely degrade M-orange and M-blue by OM-Al is about one third of that by pristine Al powder, and decreases with increasing dosage of OM-Al. The enhancement in dye removal rate by oxide modification is much better than that with ultrasonic assistance, especially for M-blue. LC/MS spectrum analyses revealed that large dye molecules are broken into small biodegradable organic molecules after reaction with OM-Al. It is deduced that the promotion of fine Al2O3 on the hydration process of Al surface passive oxide film is the main mechanism responsible for the enhancement of dye removal by OM-Al. Furthermore, OM-Al has a good recyclability and 80% of M-orange and M-blue can be removed even when it was reused for up to three cycles. These results indicate that oxide modification is an effective way to activate Al for the removal of organic dyes.
Biosorption of Methyl Blue onto tartaric acid modified wheat bran from aqueous solution
Iranian J Environ Health Sci Eng 2012 Dec 5;9(1):16.PMID:23369295DOI:10.1186/1735-2746-9-16.
Tartaric acid modified wheat bran was utilized as adsorbent to remove Methyl Blue, a basic dye from aqueous solution. Batch experiments were carried out to study the effect of various experimental parameters such as initial solution pH, contact time, initial dye concentration and adsorbent dosage, on dye adsorption. The results showed that the modification of wheat bran by tartaric acid significantly improved its adsorption capacity, and made this material a suitable adsorbent to remove Methyl Blue. The adsorption capacity of modified wheat bran was about 1.6 times higher than that of unmodified one. The amount of Methyl Blue adsorbed was found to vary with initial solution pH, adsorbent dosage, contact time and initial Methyl Blue concentration. Kinetics study showed that the overall adsorption rate of Methyl Blue was illustrated by pseudo-second-order kinetic model. The applicability of the Langmuir and Freundlich models for the data was tested. Both models adequately described the experimental data of the biosorption of Methyl Blue. The maximum adsorption capacity for Methyl Blue calculated from Langmuir model was 25.18 mg/g. The study has shown the effectiveness of modified wheat bran in the removal of Methyl Blue, and that it can be considered as an attractive alternative to the more expensive technologies used in wastewater treatment.