Methyl Green zinc chloride
(Synonyms: 甲基绿氯化锌盐； 甲基绿氯化锌盐、双绿SF、C.I.碱性蓝20；甲基绿(甲基绿氯化锌盐、双绿SF、碱性蓝20)) 目录号 : GC67838Methyl Green zinc chloride 是一种有效的荧光染料。Methyl Green zinc chloride 是细胞和电泳凝胶的 DNA 染色剂。Methyl Green zinc chloride 可以通过显微镜和流式细胞术直接测定活性，峰值在 633 和 677 nm 之间。
Sample solution is provided at 25 µL, 10mM.
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Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Methyl Green zinc chloride is a potent fluorescent dye. Methyl Green zinc chloride is a DNA stains of cells and electrophoretic gels. Methyl Green zinc chloride can be used as direct measuring of viability by both microscopy and flow cytometry, with peaks at 633 and 677 nm[1][2].
[1]. Prieto D, et, al. A fast, low cost, and highly efficient fluorescent DNA labeling method using methyl green. Histochem Cell Biol. 2014 Sep;142(3):335-45.
[2]. Kim SK, et, al. Methyl green. A DNA major-groove binding drug. FEBS Lett. 1993 Jan 2;315(1):61-4.
| Cas No. | 3/6/7114 | SDF | Download SDF |
| 别名 | 甲基绿氯化锌盐； 甲基绿氯化锌盐、双绿SF、C.I.碱性蓝20；甲基绿(甲基绿氯化锌盐、双绿SF、碱性蓝20) | ||
| 分子式 | C27H35BrCl3N3Zn | 分子量 | |
| 溶解度 | H2O : 2.5 mg/mL (ultrasonic and warming and heat to 60°C) | 储存条件 | 4°C, away from moisture |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
High-Spatial Resolution Atmospheric Pressure Mass Spectrometry Imaging Using Fiber Probe Laser Ablation-Dielectric Barrier Discharge Ionization
Anal Chem 2021 Nov 9;93(44):14694-14700.PMID:34699179DOI:10.1021/acs.analchem.1c03055.
Atmospheric pressure mass spectrometry imaging (AP-MSI) is a powerful tool in many fields; however, there are still some difficulties to achieve high spatial resolution for AP-MSI, one of them being the need for a small ablation crater. Here, a fiber probe laser ablation (FPLA) system is introduced that uses an etched optical fiber with a sharp tip (o.d. 200 nm) to deliver ablation laser pulses to a sample surface to ablate materials with high spatial resolution. The tip-to-sample distance was adjusted to ∼10 μm using a micro-actuator having a stepping motor with submicron accuracy. The laser-ablated neutrals were post-ionized using a home-built in-line dielectric barrier discharge source, which can be interfaced to any mass spectrometer with an AP interface. Using MSI on a standard sample with a striped pattern and sections of fingernails treated with the drug Methyl Green zinc chloride salt, a FPLA-DBDI-MSI spatial resolution of ≈5 μm was demonstrated.