Methylprednisolone
(Synonyms: 甲泼尼龙; U 7532) 目录号 : GC15505
甲泼尼龙是皮质类固醇,可作用于糖皮质激素受体并抑制皮质醇分泌,其IC50为1.7ng/ml。
Cas No.:83-43-2
Sample solution is provided at 25 µL, 10mM.
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Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
| Cell experiment [1]: | |
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Cell lines |
Human oligodendroglioma cell HOG |
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Preparation Method |
HOG cells were cultured in Dulbecco's Modified Eagle's Medium at 37 °C with 5% CO2. After 24 hours of cell incubation, the medium was changed to a medium containing different doses (0.5µM, 5µM, 30µM, or 50µM) of methylprednisolone for 3, 5, and 10 days. |
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Reaction Conditions |
0.5µM, 5µM, 30µM, or 50µM methylprednisolone for 3, 5, and 10 days |
|
Applications |
Methylprednisolone reduced the proliferation of HOG cells by reducing cell viability in a dose-dependent manner. HOG's death was also enhanced by methylprednisolone. In addition, methylprednisolone reduced the expression of the mature oligodendrocyte marker MBP at an early stage and inhibited myelin regeneration |
| Animal experiment [2]: | |
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Animal models |
6-week-old male C57/BL6 mice (weight 18-22 g) |
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Preparation Method |
Methylprednisolone was dissolved in saline. Mice were intragastrically administrated with methylprednisolone (25mg/kg) for 2-14 days. |
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Dosage form |
25mg/kg, intragastrically administration |
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Applications |
Methylprednisolone alleviated cognitive dysfunctions and inhibited the excessive activation of microglia in Aβ1-42-induced mice. Synaptic function-related genes were upregulated by Methylprednisolone, whereas immune response-related genes were suppressed by Methylprednisolone. |
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References: [1]. GÓmez-Pinedo U, MatÍas-Guiu JA, Ojeda-Hernandez D, de la Fuente-Martin S, Kamal OM, Benito-Martin MS, Selma-Calvo B, Montero-Escribano P, MatÍas-Guiu J. In Vitro Effects of Methylprednisolone over Oligodendroglial Cells: Foresight to Future Cell Therapies. Cells. 2023 May 30;12(11):1515. doi: 10.3390/cells12111515. PMID: 37296635; PMCID: PMC10252523. | |
Methylprednisolone is a corticosteroid that interacts with the glucocorticoid receptor and inhibits cortisol secretion with an IC50 of 1.7ng/ml [1]. Methylprednisolone exerts anti-inflammatory effects by inhibiting NF-kappaB and can be utilized in the treatment of tuberculosis and COVID-19 [2].
Methylprednisolone (0.5µM, 5µM, 30µM, or 50µM) could reduce HOG cell proliferation and promote HOG death in a dose-dependent manner. Methylprednisolone also decreased the expression of MBP, a marker for early mature oligodendrocytes, and inhibited myelin regeneration [3].
Methylprednisolone (25mg/kg) treatment significantly ameliorated l Aβ1-42-induced cognitive deficits in Alzheimer's disease mice as well as reduced microglia hyperactivation. Synaptic function-related genes could be upregulated by Methylprednisolone, while immune response-related genes were suppressed by Methylprednisolone. Methylprednisolone alleviated cognitive dysfunction by improving synaptic function and suppressing immune and inflammatory processes [4].
References:
[1]. Lew KH, Ludwig EA, Milad MA, Donovan K, Middleton E Jr, Ferry JJ, Jusko WJ. Gender-based effects on methylprednisolone pharmacokinetics and pharmacodynamics. Clin Pharmacol Ther. 1993 Oct;54(4):402-14. doi: 10.1038/clpt.1993.167. PMID: 8222483; PMCID: PMC4207261.
[2]. Li A, He Y, Yang C, Lu N, Bao J, Gao S, Hosyanto FF, He X, Fu H, Yan H, Ding N, Xu L. Methylprednisolone Promotes Mycobacterium smegmatis Survival in Macrophages through NF-κB/DUSP1 Pathway. Microorganisms. 2023 Mar 16;11(3):768. doi: 10.3390/microorganisms11030768. PMID: 36985341; PMCID: PMC10058212.
[3]. GÓmez-Pinedo U, MatÍas-Guiu JA, Ojeda-Hernandez D, de la Fuente-Martin S, Kamal OM, Benito-Martin MS, Selma-Calvo B, Montero-Escribano P, MatÍas-Guiu J. In Vitro Effects of Methylprednisolone over Oligodendroglial Cells: Foresight to Future Cell Therapies. Cells. 2023 May 30;12(11):1515. doi: 10.3390/cells12111515. PMID: 37296635; PMCID: PMC10252523.
[4]. Sun Y, Li J, A N, Li Z, Zhong W, Chen L, Liu S, Zhang B, Zhu Z, Li X. Methylprednisolone alleviates cognitive functions through the regulation of neuroinflammation in Alzheimer's disease. Front Immunol. 2023 May 1;14:1192940. doi: 10.3389/fimmu.2023.1192940. PMID: 37197654; PMCID: PMC10183590.
甲泼尼龙是皮质类固醇,可作用于糖皮质激素受体并抑制皮质醇分泌,其IC50为1.7ng/ml[1]。甲泼尼龙通过抑制NF-kappaB而发挥抗炎作用,并且可以运用于结核病及COVID-19的治疗[2]。
甲泼尼龙(0.5µM、5µM、30µM或50µM)处理人少突胶质细,可以以剂量依赖的方式减少 HOG 细胞的增殖,促进 HOG 的死亡。甲基强的松龙还降低了早期成熟少突胶质细胞标记物 MBP 的表达,抑制了髓鞘再生[3]。
甲泼尼龙(25mg/kg)治疗显著改善l Aβ1-42诱导的阿尔茨海默病小鼠的认知障碍,以及减少小胶质细胞的过度激活。突触功能相关基因可以被Methylprednisolone上调,而免疫反应相关基因则被Methylprednisolone所抑制。Methylprednisolone通过改善突触功能和抑制免疫和炎症过程来缓解认知功能障碍[4]。
| Cas No. | 83-43-2 | SDF | |
| 别名 | 甲泼尼龙; U 7532 | ||
| 化学名 | (6S,8S,9S,10R,11S,13S,14S,17R)-11,17-dihydroxy-17-(2-hydroxyacetyl)-6,10,13-trimethyl-7,8,9,11,12,14,15,16-octahydro-6H-cyclopenta[a]phenanthren-3-one | ||
| Canonical SMILES | CC1CC2C3CCC(C3(CC(C2C4(C1=CC(=O)C=C4)C)O)C)(C(=O)CO)O | ||
| 分子式 | C22H30O5 | 分子量 | 374.48 |
| 溶解度 | ≥ 15.35 mg/mL in DMSO, ≥ 9.5 mg/mL in EtOH with ultrasonic | 储存条件 | 4°C, protect from light |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 2.6704 mL | 13.3518 mL | 26.7037 mL |
| 5 mM | 0.5341 mL | 2.6704 mL | 5.3407 mL |
| 10 mM | 0.267 mL | 1.3352 mL | 2.6704 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。