Miltefosine-d4
(Synonyms: Hexadecylphosphocholine-d4, HPC-d4) 目录号 : GC49661
An internal standard for the quantification of miltefosine
Sample solution is provided at 25 µL, 10mM.
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Miltefosine-d4 is intended for use as an internal standard for the quantification of miltefosine by GC- or LC-MS. Miltefosine is an inhibitor of CTP:phosphocholine cytidylyltransferase (CCT).1 It inhibits liposome-induced CCT activity in MDCK cell homogenates when used at concentrations ranging from 10 to 50 µM, as well as induces translocation of CCT from the cell membrane to the cytosol in MDCK cells. Miltefosine inhibits phosphatidylcholine biosynthesis induced by phorbol 12-myristate 13-acetate in MDCK and HeLa cells when used at a concentration of 50 µM.2 It inhibits phosphatidylserine-activated PKC (IC50 = 62 µM), as well as PMA-induced morphological changes and proliferation of MDCK cells.3 Miltefosine is active against clinical isolate promastigotes of L. infantum (EC50s = 5-25 µM).4 Topical application of miltefosine (0.5%) completely eradicates L. amazonensis and induces re-epithelialization of lesions in a mouse model of cutaneous leishmaniasis.5 Formulations containing miltefosine have been used in the treatment of leishmaniasis and various free-living amoeba infections.
1.Geilen, C.C., Wieder, T., and Reutter, W.Hexadecylphosphocholine inhibits translocation of CTP: Choline-phosphate cytidylyltransferase in Madin-Darby canine kidney cellsJ. Biol. Chem.267(10)6719-6724(1992) 2.Wieder, T., Geilen, C.C., and Reutter, W.Antagonism of phorbol-ester-stimulated phosphatidylcholine biosynthesis by the phospholipid analogue hexadecylphosphocholineBiochem. J.291(Pt. 2)561-567(1993) 3.Geilen, C.C., Haase, R., Buchner, K., et al.The phospholipid analogue, hexadecylphosphocholine, inhibits protein kinase C in vitro and antagonises phorbol ester-stimulated cell proliferationEur. J. Cancer27(12)1650-1653(1991) 4.Espada, C.R., de Castro Levatti, E.V., BoitÉ, M.C., et al.In vitro susceptibility to miltefosine of Leishmania infantum (syn. L. chagasi) isolates from different geographical areas in BrazilMicroorganisms9(6)1228(2021) 5.Peralta, M.F., Usseglio, N.A., Bracamonte, M.E., et al.Efficacy of topical Miltefosine formulations in an experimental model of cutaneous leishmaniasisDrug Deliv. Transl. Res.12(1)180-196(2022)
| Cas No. | N/A | SDF | Download SDF |
| 别名 | Hexadecylphosphocholine-d4, HPC-d4 | ||
| Canonical SMILES | CCCCCCCCCCCCCCCCOP(OC([2H])([2H])C([2H])([2H])[N+](C)(C)C)([O-])=O | ||
| 分子式 | C21H42D4NO4P | 分子量 | 411.6 |
| 溶解度 | Methanol: soluble,Water: soluble | 储存条件 | -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 2.4295 mL | 12.1477 mL | 24.2954 mL |
| 5 mM | 0.4859 mL | 2.4295 mL | 4.8591 mL |
| 10 mM | 0.243 mL | 1.2148 mL | 2.4295 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Quality Control & SDS
- View current batch:
- Purity: >99.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet