Mitochonic acid 5 (MA-5)
(Synonyms: MA-5) 目录号 : GC31123
An inhibitor of mitochondrial dysfunction
Cas No.:1354707-41-7
Sample solution is provided at 25 µL, 10mM.
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Mitochonic acid 5 is an inhibitor of mitochondrial dysfunction.1 It increases intracellular ATP levels in Hep3B cells when used at a concentration of 3 ?M. Mitochonic acid 5 (5 ?M) rescues LPS-induced apoptosis and decreases in the mitochondrial membrane potential in BV-2 microglial cells.2 It protects against decreases in cell viability induced by L-buthionine-(S,R)-sulfoximine in fibroblasts isolated from patients with various mitochondrial diseases, including Leigh syndrome and Leber’s hereditary optic neuropathy (EC50s = 2.3-5.1 ?M), in an electron transport chain-independent manner.1 Mitochonic acid 5 (50 mg/kg) reduces plasma levels of creatinine and decreases acute renal tubular necrosis in a mouse model of bilateral retroperitoneal renal ischemia-reperfusion injury.3
1.Suzuki, T., Yamaguchi, H., Kikusato, M., et al.Mitochonic acid 5 (MA-5), a derivative of the plant hormone indole-3-acetic acid, improves survival of fibroblasts from patients with mitochondrial diseasesTohoku J. Exp. Med.236(3)225-232(2015) 2.Tan, J., Chen, S.-X., Lei, Q.-Y., et al.Mitochonic acid 5 regulates mitofusin 2 to protect microgliaNeural Regen. Res.16(9)1813-1820(2021) 3.Suzuki, T., Yamaguchi, H., Kikusato, M., et al.Mitochonic acid 5 binds mitochondria and ameliorates renal tubular and cardiac myocyte damageJ. Am. Soc. Nephrol.27(7)1925-1932(2016)
Cell experiment: | The mouse BV-2 cells used in this study are cultured in L-DMEM supplemented with 10% fetal bovine serum (FBS) at 37°C in an atmosphere with 5% CO2. To induce inflammatory injury, cells are treated with 10 ng/mL TNFα for about 12 h. Mitochonic acid 5 (0-10 μM) is incubated with BV-2 cells for about 12 h with TNFα treatment[2]. |
Animal experiment: | Mice[1]For evaluation of the blood concentrations of Mitochonic acid 5 (MA-5), Mitochonic acid 5 is orally administered at doses of 25, 50, or 150 mg/kg to C57/BL 6 mice, and blood samples are collected at the designated times. After 1 hour, the mice are euthanized. The blood concentration of Mitochonic acid 5 is determined by LC/MS/MS[1]. |
References: [1]. Suzuki T, et al. Mitochonic Acid 5 Binds Mitochondria and Ameliorates Renal Tubular and Cardiac Myocyte Damage. J Am Soc Nephrol. 2016 Jul;27(7):1925-32. | |
| Cas No. | 1354707-41-7 | SDF | |
| 别名 | MA-5 | ||
| Canonical SMILES | O=C(O)C(CC(C1=CC=C(F)C=C1F)=O)C2=CNC3=C2C=CC=C3 | ||
| 分子式 | C18H13F2NO3 | 分子量 | 329.3 |
| 溶解度 | DMSO : ≥ 106.66 mg/mL (323.90 mM) | 储存条件 | Store at -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 3.0367 mL | 15.1837 mL | 30.3674 mL |
| 5 mM | 0.6073 mL | 3.0367 mL | 6.0735 mL |
| 10 mM | 0.3037 mL | 1.5184 mL | 3.0367 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Quality Control & SDS
- View current batch:
- Purity: >99.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet