ML-031
(Synonyms: CID-2113511) 目录号 : GC11668
A S1P2 agonist
Cas No.:852230-33-2
Sample solution is provided at 25 µL, 10mM.
ML-031 is an agonist of S1P2 with EC50 value of 1 μM [1].
Sphingosine 1-phosphate (S1P) is a bioactive lysophospholipid mediator mainly released from activated platelets and implicated in many biological responses, such as cell proliferation, migration, survival, and differentiation [1]. Sphingolipids belong to a family of bioactive molecules with cell signaling properties. Sphingosine 1-phosphate (S1P) is widely expressed on various tissues and cell types. S1P is a pleiotropic lysophospholipid mediator exists in plasma and is released in large amounts from activated platelets [1]. Until now, five sphingosine-1-phosphate receptors (S1PR1-S1PR5) have been identified. S1PR4 and S1PR5 are prominently expressed in the immune and nervous systems, respectively. S1P functions by binding to five distinct G protein-coupled receptors, S1P1/EDG-1, S1P2/EDG-5, S1P3/EDG-3, S1P4/EDG-6, and S1P5/EDG-8.
ML-031 activated S1P2 with an EC50 value of 1 μM in a S1P reporter assay [1].
Reference:
[1] Satsu H, Schaeffer M T, Guerrero M, et al. A sphingosine 1-phosphate receptor 2 selective allosteric agonist[J]. Bioorganic & medicinal chemistry, 2013, 21(17): 5373-5382.
Cas No. | 852230-33-2 | SDF | |
别名 | CID-2113511 | ||
化学名 | 1-[2-[2,5-dimethyl-1-(phenylmethyl)-1H-pyrrol-3-yl]-2-oxoethyl]-2,5-pyrrolidinedione | ||
Canonical SMILES | CC1=CC(C(CN2C(CCC2=O)=O)=O)=C(C)N1CC3=CC=CC=C3 | ||
分子式 | C19H20N2O3 | 分子量 | 324.4 |
溶解度 | ≤5mg/ml in ethanol;5mg/ml in DMSO | 储存条件 | Store at -20°C |
General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效。 储备液的保存方式和期限:-80°C 储存时,请在 6 个月内使用,-20°C 储存时,请在 1 个月内使用。 为了提高溶解度,请将管子加热至37℃,然后在超声波浴中震荡一段时间。 |
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Shipping Condition | 评估样品解决方案:配备蓝冰进行发货。所有其他可用尺寸:配备RT,或根据请求配备蓝冰。 |
制备储备液 | |||
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1 mg | 5 mg | 10 mg |
1 mM | 3.0826 mL | 15.4131 mL | 30.8261 mL |
5 mM | 0.6165 mL | 3.0826 mL | 6.1652 mL |
10 mM | 0.3083 mL | 1.5413 mL | 3.0826 mL |
第一步:请输入基本实验信息(考虑到实验过程中的损耗,建议多配一只动物的药量) | ||||||||||
给药剂量 | mg/kg | 动物平均体重 | g | 每只动物给药体积 | ul | 动物数量 | 只 | |||
第二步:请输入动物体内配方组成(配方适用于不溶于水的药物;不同批次药物配方比例不同,请联系GLPBIO为您提供正确的澄清溶液配方) | ||||||||||
% DMSO % % Tween 80 % saline | ||||||||||
计算重置 |
计算结果:
工作液浓度: mg/ml;
DMSO母液配制方法: mg 药物溶于 μL DMSO溶液(母液浓度 mg/mL,
体内配方配制方法:取 μL DMSO母液,加入 μL PEG300,混匀澄清后加入μL Tween 80,混匀澄清后加入 μL saline,混匀澄清。
1. 首先保证母液是澄清的;
2.
一定要按照顺序依次将溶剂加入,进行下一步操作之前必须保证上一步操作得到的是澄清的溶液,可采用涡旋、超声或水浴加热等物理方法助溶。
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Quality Control & SDS
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- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet