ML-095 (hydrochloride)
(Synonyms: CID-25067483) 目录号 : GC10850
A PLAP inhibitor
Cas No.:1135318-57-8
Sample solution is provided at 25 µL, 10mM.
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ML-095 (hydrochloride) is a specific PLAP inhibitor [1].
Alkaline phosphatases are hydrolase enzymes responsible for removing phosphate groups from many types of molecules, including nucleotides, proteins, and alkaloids. There are three isozymes of alkaline phosphatases: placental alkaline phosphatase (PLAP), intestinal alkaline phosphatase (IAP), and tissue non-specific alkaline phosphatase (TNAP). Placental alkaline phosphatase (PLAP) is highly expressed in primate placental tissue and the biological function is still unknown. However, PLAP-like enzymes are detected in serum of patients with primary testicular tumors, in particular seminoma and other cancers [1].
ML-095 (CID-25067483) is a biochemical inhibitor of PLAP and will be useful to elucidate the key biological functions and natural substrates of human PLAP. ML095 is a specific PLAP inhibitor and inhibited PLAP, TNAP and IAP with IC50 values of 2.1, >100 and 53 μM, respectively. ML095 exhibited greater than 25-fold selectivity over tissue non-specific alkaline phosphatase (TNAP) and intestinal alkaline phosphatase (IAP) [1].
Reference:
[1]. Lanier M, Cashman J, Sergienko E, et al. Placental Alkaline Phosphatase (PLAP) Luminescent HTS assay - Probe 2.
| Cas No. | 1135318-57-8 | SDF | |
| 别名 | CID-25067483 | ||
| 化学名 | 1-(3,4-dihydroxyphenyl)-2-(2-ethyl-1H-imidazol-1-yl)-ethanone, monohydrochloride | ||
| Canonical SMILES | OC1=CC=C(C(CN2C=CN=C2CC)=O)C=C1O.Cl | ||
| 分子式 | C13H14N2O3 • HCl | 分子量 | 282.7 |
| 溶解度 | ≤10mg/ml in DMSO | 储存条件 | Store at -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 3.5373 mL | 17.6866 mL | 35.3732 mL |
| 5 mM | 0.7075 mL | 3.5373 mL | 7.0746 mL |
| 10 mM | 0.3537 mL | 1.7687 mL | 3.5373 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet