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MOCAc-PLGL(Dpa)AR Sale

目录号 : GC17832

MOCAc-PLGL(Dpa)AR 是基质金属蛋白酶 2 (MMP-2) 和 MMP-7 的带正电荧光底物。

MOCAc-PLGL(Dpa)AR Chemical Structure

Cas No.:140430-53-1

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5mg
¥2,100.00
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10mg
¥3,644.00
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Sample solution is provided at 25 µL, 10mM.

Description

We have retained the Pro-Leu-Gly-Lcu sequence, but labelled the N-terminus with the highly fluorescent (7-methoxycoumarin-4-yl) acetyl (Mea) group. The quenching Dnp group has been inserted with the novel N3-Dnp-t_-2.3-diaminopropionic acid (Dpa) in the P2' position1, as the matrix metalloproteinases favour aromatic side chains at P2'2, 3.
In the unquenched peptide Mca-Pro-Leu, the fluorescence has an excitation maximum at 328 nm and the emission is centered around 393 nm. When the fluorescence emissions were compared at a concentration of 1 ,µM, Mca-Pro-Leu was 130 times more fluorescent than Mca-Pro-Leu-Gly-Leu-Dpa-Ala-Arg-NH2.
3.3 CIeavuge of Mea-Pro-Leu-Gus)-Lrlt-Dpu-AMca-Pro-Leu-Gly-Leu-Dpa-Ala-Arg-NH2, is the most sensitive substrate so far described for continuous assays of the matrix metalloproteinases. Not only are the values of Kcut/Km, higher than those reported previously. but the cleavage product is more readily detectable, as the Mea group is both more fluorescent than tryptophan and more efficiently quenched by the Dnp group. With the exception of collegenase, continuous assays can be made with the new substrate at enzyme concentrations similar to those required in assays with their preferred macromolecular substrates. Mca-Pro- Leu-Gly-Leu-Dpa-Ala-Arg-NH2 is suitable therefore for the assessment of total matrix metalloproteinase activity in crude preparations. Further work to develop peptides that are specifically cleaved by the individual enzymes will permit their measurement under these conditions3.

Reference:
1.    Schectcr, I, and Beryer. A. (lYG7) Biochem. Biophyt;. Rcs, Commun. 27, 157-161
2.    Nctzcl-Arnctt, s.. Mallya. SK., Nugasc. H.. Birkedal-Hansen, H. and Van Wurt. H.E. (1991) Anal. Biochcm. 195. 86-92.
3.    Netzel-Arnctt, S., Fields. G., Birkedal-Hunscn. H. and Vai Wart. H.E. (1991) J. Biol. Chem. 266. 6747-6755
4.    C.G.Knight, F. Willenbrock and G. Murphy. A novel coumarin-labelled peptide for sensitive continuous assays of the matrix metalloproteinases. FEDS. Volume 296, number 3, 263-266

化学性质

Cas No. 140430-53-1 SDF
化学名 (2R)-N-((6S,9S,12S,16R,22R)-1,12-diamino-6-carbamoyl-13-(2,4-dinitrophenyl)-1-imino-16-isobutyl-9,24-dimethyl-8,11,15,18,21-pentaoxo-2,7,10,14,17,20-hexaazapentacosan-22-yl)-1-(2-(7-methoxy-2-oxo-2H-chromen-4-yl)acetyl)pyrrolidine-2-carboxamide
Canonical SMILES COC1=CC=C(C(CC(N2CCC[C@@H]2C(N[C@H](CC(C)C)C(NCC(N[C@H](CC(C)C)C(NC([C@H](C(N[C@@H](C(N[C@@H](C(N)=O)CCCNC(N)=N)=O)C)=O)N)C3=CC=C([N+]([O-])=O)C=C3[N+]([O-])=O)=O)=O)=O)=O)=O)=CC(O4)=O)C4=C1
分子式 C49H68N14O15 分子量 1093.15
溶解度 DMSO : 100 mg/mL (91.48 mM; ultrasonic and warming and heat to 60°C) 储存条件 Store at -20°C
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储备液的保存方式和期限:-80°C 储存时,请在 6 个月内使用,-20°C 储存时,请在 1 个月内使用。
为了提高溶解度,请将管子加热至37℃,然后在超声波浴中震荡一段时间。
Shipping Condition 评估样品解决方案:配备蓝冰进行发货。所有其他可用尺寸:配备RT,或根据请求配备蓝冰。

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1 mg 5 mg 10 mg
1 mM 0.9148 mL 4.5739 mL 9.1479 mL
5 mM 0.183 mL 0.9148 mL 1.8296 mL
10 mM 0.0915 mL 0.4574 mL 0.9148 mL
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