Msr-blue
目录号 : GC61553
Msr-blue是首个"Turn-on"型的甲硫氨酸亚砜还原酶(methioninesulfoxidereductase)荧光探针，具有超过100倍的荧光增量。Msr-blue用于监测活细胞中甲硫氨酸亚砜还原酶的活性(Λex=340nm,Λem=440nm)。
Cas No.:2966537-39-1
Sample solution is provided at 25 µL, 10mM.
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Msr-blue is a first turn-on fluorescent probe for methionine sulfoxide reductase with a more than 100-fold fluorescence increment. Msr-blue is used for monitoring the enzyme activity in live cells (Λex=340 nm, Λem=440 nm)[1].
Msr-blue is emited blue fluorescence after activation by methionine sulfoxide reductase A (Msr A). Msr-blue responded to Msr A in both a time- and dose-dependent manner, and more than a 100-fold increase in the emission is observed. Msr-blue is converted to its corresponding sulfide (15′) under catalysis by either the purified Msr A or a cell lysate[1]. The 6-OHDA-treated PC12 cells as a cellular model of Parkinson's disease (PD) is employed and applied Msr-blue to probe the function of Msrs in the cells. With the aid of Msr-blue, a decline of the Msr activity in a PD model was disclosed for the first time[1].
[1]. Liangwei Zhang, et al. A specific fluorescent probe reveals compromised activity of methionine sulfoxide reductases in Parkinson's disease. Chem Sci. 2017 Apr 1;8(4):2966-2972.
| Cas No. | 2966537-39-1 | SDF | |
| Canonical SMILES | CC(C1=CC=C(C=C1O2)S(C)=O)=CC2=O | ||
| 分子式 | C11H10O3S | 分子量 | 222.26 |
| 溶解度 | DMSO : 25 mg/mL (112.48 mM; Need ultrasonic) | 储存条件 | Store at -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 4.4992 mL | 22.4962 mL | 44.9924 mL |
| 5 mM | 0.8998 mL | 4.4992 mL | 8.9985 mL |
| 10 mM | 0.4499 mL | 2.2496 mL | 4.4992 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet