Msr-Ratio
(Synonyms: Msr-green) 目录号 : GC61557
Msr-Ratio(Msr-green)是一种比率型甲硫氨酸亚砜还原酶(methioninesulfoxidereductase)的荧光探针(Λex=375nm,Λem=550nm)。Msr-Ratio可用于监测体外和活细胞中的甲硫氨酸亚砜还原酶活性。
Cas No.:2290635-22-0
Sample solution is provided at 25 µL, 10mM.
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Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Msr-Ratio (Msr-green) is a ratiometric fluorescent probe of methionine sulfoxide reductase (Λex=375 nm, Λem=550 nm). Msr-Ratio is used for monitoring the enzyme activity in vitro and in live cells[1].
Msr-Ratio is converted to its corresponding sulfide form by methionine sulfoxide reductase. Msr-Ratio displays favorable properties such as a nearly 400-fold fluorescence change, fast response rate (<30 min), large Stokes shift (120 nm), and green emission (550 nm)[1].There is a very weak background signal in live HL60 cells after the addition of Msr-Ratio. As the Msr-Ratio (10 μM) incubation time increased in cells from 0 to 8 h, the fluorescence signal constantly strengthened[1].
[1]. Liangwei Zhang, et al. A ratiometric fluorescent probe of methionine sulfoxide reductase with an improved response rate and emission wavelength. Chem Commun (Camb). 2019 Jan 29;55(10):1502-1505.
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 3.222 mL | 16.1098 mL | 32.2196 mL |
| 5 mM | 0.6444 mL | 3.222 mL | 6.4439 mL |
| 10 mM | 0.3222 mL | 1.611 mL | 3.222 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。