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Myclobutanil Sale

(Synonyms: 腈菌唑) 目录号 : GC30867

An Analytical Reference Standard

Myclobutanil Chemical Structure

Cas No.:88671-89-0

规格 价格 库存 购买数量
10mM (in 1mL DMSO)
¥589.00
现货
100mg
¥536.00
现货

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Sample solution is provided at 25 µL, 10mM.

Description

Myclobutanil is an analytical reference standard categorized as a triazole fungicide.1 It has been found as a contaminant in concentrated Cannabis extracts from the California medical Cannabis market. This product is intended for research and forensic applications.

1.Raber, J.C., Elzinga, S., and Kaplan, C.Understanding dabs: Contamination concerns of cannabis concentrates and cannabinoid transfer during the act of dabbingJ. Toxicol. Sci.40(6)797-803(2015)

实验参考方法

Kinase experiment:

To further evaluate apoptosis, cell extracts are collected after 24 h of exposure to Myclobutanil, centrifuged, and analyzed with a multiplex biometric ELISA-based immunoassay containing dyed microspheres conjugated to a monoclonal antibody specific for the target protein. Apoptosis biomarkers are BCL-xL/Bak dimer and Mcl-1/Bak dimer, quantified using RBM Apoptosis Panel 3. Each experiment is performed in triplicate and apoptosis biomarker levels determined using the Bio-Plex Array Reader. The analytic concentrations are calculated using a standard curve, according to the manufacturer’s instructions[1].

Cell experiment:

The hepatoma cell line HepG2 is used in this study. The cells are grown on tissue culture plates in an incubator with a humidified atmosphere (95% air/5% CO2 v/v) at 37°C. Steatosis is induced by incubating the hepatocytes with 6 mM of a 1:1 v/v mixture of oleic (18:1) and linoleic (18:2) fatty acids (Fas) for 24 h. After a wash with PBS, cells are exposed for an additional 24 h to Myclobutanil at 0.1, 1, 10, 100 or 500 ppm. Cytotoxicity is assessed in HepG2 cells (1.0×105 cells/well in 24-well plates) by measuring the reduction of the tetrazolium dye 3-(4, 5-dimethylthiazol-2-yl)-5-(3carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTT)[1].

References:

[1]. Stellavato A, et al. Myclobutanil worsens nonalcoholic fatty liver disease: An in vitro study of toxicity and apoptosis on HepG2 cells. Toxicol Lett. 2016 Nov 16;262:100-104.

化学性质

Cas No. 88671-89-0 SDF
别名 腈菌唑
Canonical SMILES N#CC(C1=CC=C(Cl)C=C1)(CCCC)CN2N=CN=C2
分子式 C15H17ClN4 分子量 288.78
溶解度 DMSO : 103 mg/mL (356.67 mM) 储存条件 Store at -20°C
General tips 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效。
储备液的保存方式和期限:-80°C 储存时,请在 6 个月内使用,-20°C 储存时,请在 1 个月内使用。
为了提高溶解度,请将管子加热至37℃,然后在超声波浴中震荡一段时间。
Shipping Condition 评估样品解决方案:配备蓝冰进行发货。所有其他可用尺寸:配备RT,或根据请求配备蓝冰。

溶解性数据

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1 mg 5 mg 10 mg
1 mM 3.4628 mL 17.3142 mL 34.6284 mL
5 mM 0.6926 mL 3.4628 mL 6.9257 mL
10 mM 0.3463 mL 1.7314 mL 3.4628 mL
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