N-Arachidonoyl Taurine-d4
目录号 : GC47749
A neuropeptide with diverse biological activities
Cas No.:119959-65-8
Sample solution is provided at 25 µL, 10mM.
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Quality Control & SDS
- View current batch:
- Purity: >99.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
N-Arachidonoyl taurine-d4 is intended for use as an internal standard for the quantification of N-arachidonoyl taurine by GC- or LC-MS. N-Arachidonoyl taurine is an arachidonoyl amino acid.1 It is oxygenated by 12(S)- and 15(S)-lipoxygenase and is converted to 12-HETE-taurine (12-HETE-T) in murine resident peritoneal macrophages.2 N-Arachidonoyl taurine is an activator of the transient receptor potential vanilloid (TRPV) channels TRPV1 and TRPV4 (EC50s = 28 and 21 µM, respectively).1 It increases calcium flux in HIT-T15 pancreatic β-cells and INS-1 rat islet cells when used at a concentration of 10 µM and increases insulin secretion from 832/13 INS-1 pancreatic β-cells.3 The levels of N-arachidonoyl taurine are changed in mouse brain following administration of δ9-tetrahydrocannabinol (δ9-THC).4
1.Saghatelian, A., McKinney, M.K., Bandell, M., et al.A FAAH-regulated class of N-acyl taurines that activates TRP ion channelsBiochemistry45(30)9007-9015(2006) 2.Turman, M.V., Kingsley, P.J., Rouzer, C.A., et al.Oxidative metabolism of a fatty acid amide hydrolase-regulated lipid, arachidonoyltaurineBiochemistry47(12)3917-3925(2008) 3.Waluk, D.P., Vielfort, K., Derakhshan, S., et al.N-Acyl taurines trigger insulin secretion by increasing calcium flux in pancreatic β-cellsBiochem. Biophys. Res. Commun.430(1)54-59(2013) 4.Leishman, E., Murphy, M., Mackie, K., et al.δ9-Tetrahydrocannabinol changes the brain lipidome and transcriptome differentially in the adolescent and the adultBiochim. Biophys. Acta. Mol. Cell Biol. Lipids1863(5)479-492(2018)
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 2.4062 mL | 12.0308 mL | 24.0616 mL |
| 5 mM | 0.4812 mL | 2.4062 mL | 4.8123 mL |
| 10 mM | 0.2406 mL | 1.2031 mL | 2.4062 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。