N-hydroxylamine Dapsone
(Synonyms: 羟胺氨苯砜) 目录号 : GC52007
An active metabolite of dapsone
Cas No.:32695-27-5
Sample solution is provided at 25 µL, 10mM.
Quality Control & SDS
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- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
N-hydroxylamine Dapsone is an active metabolite of dapsone .1 It is formed via N-hydroxylation of dapsone by the cytochrome P450 (CYP) isoforms CYP2C8, CYP2C9, CYP2C18, and CYP2C19 in the liver.2 N-hydroxylamine Dapsone is cytotoxic to rat hepatocytes (LC50 = 3.6 mM).3 It induces hemolysis in isolated human erythrocytes at a concentration of 100 µM.1 N-hydroxylamine Dapsone induces phosphatidylserine (PS) exposure on the outer membrane, a marker of apoptosis and platelet activation, and increases the production of reactive oxygen species (ROS) in a concentration-dependent manner in isolated human erythrocytes. It increases thrombus weight in a rat model of surgically induced thrombosis when administered at a single dose of 50 mg/kg or at a dose of 10 mg/kg per day for four days.
1.Bian, Y., Kim, K., An, G.-J., et al.Dapsone hydroxylamine, an active metabolite of dapsone, can promote the procoagulant activity of red blood cells and thrombosisToxicol. Sci.172(2)435-444(2019) 2.Winter, H.R., Wang, Y., and Unadkat, J.D.CYP2C8/9 mediate dapsone N-hydroxylation at clinical concentrations of dapsoneDrug Metab. Dispos.28(8)865-868(2000) 3.Veggi, L.M., Pretto, L., Ochoa, E.J., et al.Dapsone induces oxidative stress and impairs antioxidant defenses in rat liverLife Sci.83(5-6)155-163(2008)
Cas No. | 32695-27-5 | SDF | Download SDF |
别名 | 羟胺氨苯砜 | ||
Canonical SMILES | O=S(C1=CC=C(C=C1)N)(C2=CC=C(NO)C=C2)=O | ||
分子式 | C12H12N2O3S | 分子量 | 264.3 |
溶解度 | DMSO: soluble | 储存条件 | -20°C |
General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效。 储备液的保存方式和期限:-80°C 储存时,请在 6 个月内使用,-20°C 储存时,请在 1 个月内使用。 为了提高溶解度,请将管子加热至37℃,然后在超声波浴中震荡一段时间。 |
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Shipping Condition | 评估样品解决方案:配备蓝冰进行发货。所有其他可用尺寸:配备RT,或根据请求配备蓝冰。 |
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1 mg | 5 mg | 10 mg |
1 mM | 3.7836 mL | 18.9179 mL | 37.8358 mL |
5 mM | 0.7567 mL | 3.7836 mL | 7.5672 mL |
10 mM | 0.3784 mL | 1.8918 mL | 3.7836 mL |
第一步:请输入基本实验信息(考虑到实验过程中的损耗,建议多配一只动物的药量) | ||||||||||
给药剂量 | mg/kg | 动物平均体重 | g | 每只动物给药体积 | ul | 动物数量 | 只 | |||
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% DMSO % % Tween 80 % saline | ||||||||||
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计算结果:
工作液浓度: mg/ml;
DMSO母液配制方法: mg 药物溶于 μL DMSO溶液(母液浓度 mg/mL,
体内配方配制方法:取 μL DMSO母液,加入 μL PEG300,混匀澄清后加入μL Tween 80,混匀澄清后加入 μL saline,混匀澄清。
1. 首先保证母液是澄清的;
2.
一定要按照顺序依次将溶剂加入,进行下一步操作之前必须保证上一步操作得到的是澄清的溶液,可采用涡旋、超声或水浴加热等物理方法助溶。
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