N-(p-amylcinnamoyl) Anthranilic Acid
(Synonyms: 2-(3-(4-戊基苯基)丙烯酰胺基)苯甲酸,ACA) 目录号 : GC13930
A TRP channel blocker and PLA2 inhibitor
Cas No.:110683-10-8
Sample solution is provided at 25 µL, 10mM.
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N-(p-amylcinnamoyl) Anthranilic Acid (ACA) is a broad-spectrum phospholipase A2 (PLA2) inhibitor and TRP channel blocker [1] [2].
PLA2 enzymes are a family of structurally different enzymes including at least nine subfamilies. The mammalian TRP proteins including at least 27 members and can be grouped into TRPC, TRPV, TRPM, TRPA, TRPP, and TRPML six subfamilies [1].
N-(p-amylcinnamoyl) Anthranilic Acid (ACA) is a broad-spectrum PLA2 inhibitor and TRP channel blocker [1] [2]. In HEK293 cells transfected with human TRPM2, ACA (20 ?M) completely blocked H2O2-induced Ca2+ signals and ADPR-induced whole-cell currents with IC50 value of 1.7 ?M. ACA (20 ?M) also blocked human TRPM8-induced Ca2+ signals and TRPC6-mediated currents with IC50 values of 3.9 ?M and 2.3 ?M, respectively. ACA can serve as a useful tool for studying the function of TRPM2 in native cells [2]. In isolated pig cardiac ventricular myocytes, ACA reversibly inhibited ICl(Ca) with IC50 value of 4.2 ?M in a concentration-dependent way. ACA (50 ?M) also inhibited the cAMP-activated chloride current.
References:
[1].Harteneck C, Frenzel H, Kraft R. N-(p-amylcinnamoyl)anthranilic acid (ACA): a phospholipase A(2) inhibitor and TRP channel blocker. Cardiovasc Drug Rev. 2007 Spring;25(1):61-75.
[2].Kraft R, Grimm C, Frenzel H, et al. Inhibition of TRPM2 cation channels by N-(p-amylcinnamoyl)anthranilic acid. Br J Pharmacol. 2006 Jun;148(3):264-73.
[3].Gwanyanya A, Macianskiene R, Bito V, et al. Inhibition of the calcium-activated chloride current in cardiac ventricular myocytes by N-(p-amylcinnamoyl)anthranilic acid (ACA). Biochem Biophys Res Commun. 2010 Nov 19;402(3):531-6.
| Cas No. | 110683-10-8 | SDF | |
| 别名 | 2-(3-(4-戊基苯基)丙烯酰胺基)苯甲酸,ACA | ||
| 化学名 | 2-((Z)-((E)-1-hydroxy-3-(4-pentylphenyl)allylidene)amino)benzoic acid | ||
| Canonical SMILES | CCCCCC1=CC=C(/C([H])=C([H])/C(O)=N/C2=CC=CC=C2C(O)=O)C=C1 | ||
| 分子式 | C21H23NO3 | 分子量 | 337.41 |
| 溶解度 | ≥ 33.7mg/mL in DMSO | 储存条件 | Store at -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 2.9638 mL | 14.8188 mL | 29.6375 mL |
| 5 mM | 0.5928 mL | 2.9638 mL | 5.9275 mL |
| 10 mM | 0.2964 mL | 1.4819 mL | 2.9638 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet