N6-Methyladenosine 5'-monophosphate (sodium salt)
(Synonyms: N6-甲基腺苷5′-单磷酸酯钠盐,N6-Methyl-AMP) 目录号 : GC18197N6-Methyladenosine 5'-monophosphate (sodium salt)是糖原磷酸化酶b的激活剂(Ka=22µM)和大鼠腺苷酸激酶II 的非竞争性抑制剂(Ki=4.2mM)。
Cas No.:81921-35-9
Sample solution is provided at 25 µL, 10mM.
N6-Methyladenosine 5'-monophosphate (sodium salt) is an activator of glycogen phosphorylase b (Ka=22µM)[1] and a non-competitive inhibitor of rat adenylate kinase II (Ki=4.2mM)[2]. N6-Methyladenosine 5'-monophosphate (sodium salt) is the 5'-monophosphate form of N6-methyladenosine (m6A), usually in the form of a sodium salt. N6-methyladenosine (m6A) is a methylated adenosine molecule in which the N6 position of adenine is methylated. m6A, a common form of RNA modification, mainly exists in mRNA and affects its stability, translation efficiency and gene expression regulation[3]. N6-Methyladenosine 5'-monophosphate (sodium salt) is often used as a research tool to investigate the mechanism of action of m6A modification in different biological processes. N6-Methyladenosine 5'-monophosphate (sodium salt) is primarily used in research on RNA epigenetics, particularly for studying the mechanisms of m6A modification[4][5], RNA-protein interactions[6], and its role in gene expression regulation[7].
References:
[1] Groner, Y., Grosfeld, H., & Littauer, U. Z. (1976). 5'-Capping structures of Artemia salina mRNA and the translational inhibition by cap analogs. European journal of biochemistry, 71(1), 281–293.
[2] Hai, T. T., Abo, M., & Hampton, A. (1982). Species- or isozyme-specific enzyme inhibitors. 9. Selective effects in inhibitions of rat pyruvate kinase isozymes by adenosine 5'-diphosphate derivatives. Journal of medicinal chemistry, 25(10), 1184–1188.
[3] Liu, Y., Yang, D., Liu, T., Chen, J., Yu, J., & Yi, P. (2023). N6-methyladenosine-mediated gene regulation and therapeutic implications. Trends in molecular medicine, 29(6), 454–467.
[4] Chen, Y., Wu, Y., Zhu, L., Chen, C., Xu, S., Tang, D., Jiao, Y., & Yu, W. (2022). METTL3-Mediated N6-Methyladenosine Modification of Trim59 mRNA Protects Against Sepsis-Induced Acute Respiratory Distress Syndrome. Frontiers in immunology, 13, 897487.
[5] Yu, J., Li, Y., Wang, T., & Zhong, X. (2018). Modification of N6-methyladenosine RNA methylation on heat shock protein expression. PloS one, 13(6), e0198604.
[6] Fry, N. J., Law, B. A., Ilkayeva, O. R., Holley, C. L., & Mansfield, K. D. (2017). N6-methyladenosine is required for the hypoxic stabilization of specific mRNAs. RNA (New York, N.Y.), 23(9), 1444–1455.
[7] Nagarajan, A., Janostiak, R., & Wajapeyee, N. (2019). Dot Blot Analysis for Measuring Global N6-Methyladenosine Modification of RNA. Methods in molecular biology (Clifton, N.J.), 1870, 263–271.
N6-Methyladenosine 5'-monophosphate (sodium salt)是糖原磷酸化酶b的激活剂(Ka=22µM)[1] 和大鼠腺苷酸激酶II 的非竞争性抑制剂(Ki=4.2mM)[2]。N6-Methyladenosine 5'-monophosphate (sodium salt) 是N6-甲基腺苷(m6A)的5'-单磷酸形式,通常以钠盐的形式存在。N6-甲基腺苷(m6A)是一种甲基化的腺苷分子,其中腺嘌呤的N6位置被甲基化。m6A是一种常见的RNA修饰形式,主要存在于mRNA中,影响mRNA的稳定性、翻译效率和基因表达调控[3]。N6-Methyladenosine 5'-monophosphate (sodium salt) 常被用作研究m6A修饰在不同生物过程中的作用机制的研究工具。N6-Methyladenosine 5'-monophosphate (sodium salt) 主要用于研究RNA表观遗传学,特别是m6A修饰机制[4][5]、RNA-蛋白质相互作用[6]及其在基因表达调控中的作用[7]。
Cas No. | 81921-35-9 | SDF | |
别名 | N6-甲基腺苷5′-单磷酸酯钠盐,N6-Methyl-AMP | ||
化学名 | N-methyl-5'-adenylic acid, disodium salt | ||
Canonical SMILES | CNC1=NC=NC2=C1N=CN2[C@H]3[C@H](O)[C@H](O)[C@@H](COP([O-])([O-])=O)O3.[Na+].[Na+] | ||
分子式 | C11H14N5O7P • 2Na | 分子量 | 405.2 |
溶解度 | 10mg/mL in PBS, pH 7.2 | 储存条件 | Store at -20°C |
General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效。 储备液的保存方式和期限:-80°C 储存时,请在 6 个月内使用,-20°C 储存时,请在 1 个月内使用。 为了提高溶解度,请将管子加热至37℃,然后在超声波浴中震荡一段时间。 |
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Shipping Condition | 评估样品解决方案:配备蓝冰进行发货。所有其他可用尺寸:配备RT,或根据请求配备蓝冰。 |
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1 mg | 5 mg | 10 mg |
1 mM | 2.4679 mL | 12.3396 mL | 24.6792 mL |
5 mM | 0.4936 mL | 2.4679 mL | 4.9358 mL |
10 mM | 0.2468 mL | 1.234 mL | 2.4679 mL |
第一步:请输入基本实验信息(考虑到实验过程中的损耗,建议多配一只动物的药量) | ||||||||||
给药剂量 | mg/kg | 动物平均体重 | g | 每只动物给药体积 | ul | 动物数量 | 只 | |||
第二步:请输入动物体内配方组成(配方适用于不溶于水的药物;不同批次药物配方比例不同,请联系GLPBIO为您提供正确的澄清溶液配方) | ||||||||||
% DMSO % % Tween 80 % saline | ||||||||||
计算重置 |
计算结果:
工作液浓度: mg/ml;
DMSO母液配制方法: mg 药物溶于 μL DMSO溶液(母液浓度 mg/mL,
体内配方配制方法:取 μL DMSO母液,加入 μL PEG300,混匀澄清后加入μL Tween 80,混匀澄清后加入 μL saline,混匀澄清。
1. 首先保证母液是澄清的;
2.
一定要按照顺序依次将溶剂加入,进行下一步操作之前必须保证上一步操作得到的是澄清的溶液,可采用涡旋、超声或水浴加热等物理方法助溶。
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