NADH disodium salt
(Synonyms: beta-烟酰胺腺嘌呤二核苷二钠,Disodium NADH) 目录号 : GC61830
NADH disodium salt是烟酰胺腺嘌呤二核苷酸磷酸(NADH)的一种钠盐形式,NADH是一种辅酶,在细胞能量代谢中作为再生电子供体,包括糖酵解、β氧化和柠檬酸循环。
Cas No.:606-68-8
Sample solution is provided at 25 µL, 10mM.
;)
,-1-7$$$37316672.jpg');)
;)
;)
$$$36806353.jpg');)
;33(7)%EF%BC%9A546-561$$$37156877.jpg');)
;)
;)
;)
%201124-1138.$$$35908550.jpg');)
%20766-780.$$$37100057.jpg');)
%20418-432.$$$36893736.jpg');)
%EF%BC%9A-240-255.$$$35108512.jpg');)
533-545$$$36543525.jpg');)
%201-13.$$$36600053.jpg');)
;)
NADH disodium salt is the sodium salt form of nicotinamide adenine dinucleotide phosphate (NADH), a coenzyme that serves as a regenerative electron donor in cellular energy metabolism, including glycolysis, beta oxidation, and citric acid Loop[1]. NADH can be used for the synthesis and regeneration of tetrahydrobiopterin (BH4), which is an essential cofactor for the rate-limiting enzyme in dopamine (DA) synthesis [2].
In vitro, NADH disodium salt (1mM) treated C2C12, Neuro2a and AML12 cells for 24h, significantly increased the NAD+ production of the three cells and protected the cells from genotoxicity [3]. NADH disodium salt (100µM) treated HUVEC and PIEC cells for 30 minutes had no significant effect on intracellular O2- production [4]. When treatment with NADH disodium salt (100µM) induces rodent β-cells, it cannot enhance depolarization-induced exocytosis [5].
In vivo, subcutaneous injection of NADH disodium salt (10 or 100 mg/kg) in SD rats did not affect the tissue contents of DA, 5-HT and their metabolites in the midbrain or striatum [2]. When treated with intraperitoneal injection of NADH disodium salt (1000mg/kg) in C57BL/6J mice, the NAD+ content in the blood, brain, fat and kidney increased several times, and the NAD+ content in the liver increased by more than 7 times within 4 hours [3]. Intraperitoneal injection of NADH disodium salt (5µM) into ICR mice significantly increased the urinary excretion of nicotinamide and its metabolites [6].
References:
[1] Ying W. NAD+ and NADH in cellular functions and cell death[J]. Front Biosci, 2006, 11(1): 3129-3148.
[2]Pearl S M, Antion M D, Stanwood G D, et al. Effects of NADH on dopamine release in rat striatum[J]. Synapse, 2000, 36(2): 95-101.
[3] Wu K, Li J, Zhou X, et al. NADH and NRH as potential dietary supplements or pharmacological agents for early liver injury caused by acute alcohol exposure[J]. Journal of Functional Foods, 2021, 87: 104852.
[4] Li,J. Differential NADPH-versus NADH-dependent superoxide production by phagocyte-type endothelial cell NADPH oxidase[J].Cardiovascular Research, 2001, 52(3):477.
[5] Ivarsson R, Quintens R, Dejonghe S, et al. Redox control of exocytosis: regulatory role of NADPH, thioredoxin, and glutaredoxin[J]. Diabetes, 2005, 54(7): 2132-2142.
[6]Kimura N, Fukuwatari T, Sasaki R, et al. Comparison of metabolic fates of nicotinamide, NAD+ and NADH administered orally and intraperitoneally; characterization of oral NADH[J]. Journal of nutritional science and vitaminology, 2006, 52(2): 142-148.
NADH disodium salt是烟酰胺腺嘌呤二核苷酸磷酸(NADH)的一种钠盐形式,NADH是一种辅酶,在细胞能量代谢中作为再生电子供体,包括糖酵解、β氧化和柠檬酸循环[1]。NADH可用于四氢生物蝶呤(BH4)的合成和再生,BH4又是多巴胺(DA)合成限速酶的必需辅因子[2]。
在体外,NADH disodium salt(1mM)处理C2C12, Neuro2a和AML12细胞24h,显著增加了三种细胞的NAD+的产量并保护细胞免受遗传毒性[3]。NADH disodium salt(100µM)处理HUVEC和PIEC细胞30分钟,对细胞内的O2-产量没有显著影响[4]。NADH disodium salt(100µM)处理诱导啮齿动物β细胞时,不能增强去极化诱发的胞吐作用[5]。
在体内,NADH disodium salt(10或100 mg/kg)皮下注射处理SD大鼠,不影响中脑或纹状体中DA、5-HT及其代谢产物的组织含量[2]。NADH disodium salt(1000mg/kg)腹膜内注射处理C57BL/6J小鼠,血液、脑、脂肪和肾脏中NAD+含量增加数倍,肝脏中4h内超过7倍[3]。NADH disodium salt(5µM)腹腔注射处理ICR小鼠,显著增加了体内烟酰胺及其代谢物的尿排泄[6]。
| Cell experiment [1]: | |
|
Cell lines |
C2C12, Neuro2a and AML12 cells |
|
Preparation Method |
C2C12, Neuro2a and AML12 cells were incubated with 1 mM NADH disodium salt or NRH for 24 h. |
|
Reaction Conditions |
1mM; 24 h |
|
Applications |
NADH disodium salt increases the NAD+ concentration to reduce toxicity, and can protect cells in genotoxicity environmen. |
| Animal experiment [2]: | |
|
Animal models |
Male SD rats |
|
Preparation Method |
NADH disodium salt (10 or 100 mg/kg) was freshly dissolved in sterile 0.9% saline and administered subcutaneously. Rats were decapitated 60 minutes later, and the striatum and midbrain were dissected on ice and homogenized in 1.3 or 0.65 ml of 0.1 NHClO4, respectively. |
|
Dosage form |
10 or 100mg/kg；s.c. |
|
Applications |
NADH disodium salt did not influence the tissue content of DA, 5-HT, or their metabolites in the midbrain or striatum. |
|
References: [1] Wu K, Li J, Zhou X, et al. NADH and NRH as potential dietary supplements or pharmacological agents for early liver injury caused by acute alcohol exposure[J]. Journal of Functional Foods, 2021, 87: 104852. | |
| Cas No. | 606-68-8 | SDF | |
| 别名 | beta-烟酰胺腺嘌呤二核苷二钠,Disodium NADH | ||
| Canonical SMILES | NC1=C2C(N([C@H]3[C@H](O)[C@H](O)[C@@H](COP(OP([O-])(OC[C@@H]4[C@@H](O)[C@@H](O)[C@H](N5C=C(C(N)=O)CC=C5)O4)=O)([O-])=O)O3)C=N2)=NC=N1.[Na+].[Na+] | ||
| 分子式 | C21H27N7Na2O14P2 | 分子量 | 709.4 |
| 溶解度 | Water : >100 mg/mL (140.96 mM) | 储存条件 | Store at 2-8°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
||
| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
 |
1 mg | 5 mg | 10 mg |
| 1 mM | 1.4096 mL | 7.0482 mL | 14.0964 mL |
| 5 mM | 0.2819 mL | 1.4096 mL | 2.8193 mL |
| 10 mM | 0.141 mL | 0.7048 mL | 1.4096 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Quality Control & SDS
- View current batch:
- Purity: >99.50%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet