NADPH tetrasodium salt
(Synonyms: 还原型辅酶Ⅱ四钠) 目录号 : GC34058
NADPH tetrasodium salt是还原型烟酰胺腺嘌呤二核苷酸磷酸（NADPH）的一种钠盐形式，NADPH是细胞内必需的辅因子，充当电子供体。
Cas No.:2646-71-1
Sample solution is provided at 25 µL, 10mM.
NADPH tetrasodium salt is a sodium salt form of reduced nicotinamide adenine dinucleotide phosphate (NADPH), which is an essential cofactor in cells and acts as an electron donor[1]. NADPH is a reduced coenzyme II that participates in many biosynthetic metabolic pathways, such as fatty acid synthesis, cholesterol synthesis, oxalic acid cycle, etc.[1]. NADPH tetrasodium salt is a metabolite produced in Escherichia coli (strain K12, MG 1655)[2].
In vitro, NADPH tetrasodium salt (100µM) treated HUVEC and PIEC cells for 30 minutes, significantly increasing the O2− production of both cells[3]. Adding NADPH tetrasodium salt (100µM) to re-concentrated pulmonary artery homogenate significantly increased the activity of soluble guanylate cyclase (sGC), increasing the basal activity by 2.2±1.0 times[4]. Treatment with NADPH tetrasodium salt (0-300µM) induces rodent β-cells and dose-dependently induces Ca2+-dependent exocytosis[5].
In vivo, intravenous injection of NADPH tetrasodium salt (16 mg/kg) in male SD rats resulted in a significant increase in +dp/dt and a significant decrease in -dp/dt within 60 minutes after administration, which enhanced the cardiac function in rats [2]. Intravenous injection of NADPH tetrasodium salt (2.5 mg/kg) treated rat and mouse stroke models, significantly reducing cerebral infarction volume, improving neurological function, and increasing survival rate [6].
References:
[1]Qian K, Tang J, Ling Y J, et al. Exogenous NADPH exerts a positive inotropic effect and enhances energy metabolism via SIRT3 in pathological cardiac hypertrophy and heart failure[J]. EBioMedicine, 2023, 98.
[2]Chou H H, Marx C J, Sauer U. Transhydrogenase promotes the robustness and evolvability of E. coli deficient in NADPH production[J]. PLoS genetics, 2015, 11(2): e1005007.
[3] Li,J. Differential NADPH-versus NADH-dependent superoxide production by phagocyte-type endothelial cell NADPH oxidase[J].Cardiovascular Research, 2001, 52(3):477.
[4] Gupte S A, Rupawalla T, Phillibert D, et al. NADPH and heme redox modulate pulmonary artery relaxation and guanylate cyclase activation by NO[J]. Am J Physiol, 1999, 277:1124-32.
[5] Ivarsson R, Quintens R, Dejonghe S, et al. Redox control of exocytosis: regulatory role of NADPH, thioredoxin, and glutaredoxin[J]. Diabetes, 2005, 54(7): 2132-2142.
[6]Mei,Li,Zhi-Peng,et al.Reduced Nicotinamide Adenine Dinucleotide Phosphate, a Pentose Phosphate Pathway Product, Might Be a Novel Drug Candidate for Ischemic Stroke.[J].Stroke A Journal of Cerebral Circulation, 2016.
NADPH tetrasodium salt是还原型烟酰胺腺嘌呤二核苷酸磷酸（NADPH）的一种钠盐形式，NADPH是细胞内必需的辅因子，充当电子供体[1]。NADPH是一种还原型辅酶Ⅱ，参与许多生物合成代谢途径，如脂肪酸合成、胆固醇合成、草酸循环等[1]。NADPH tetrasodium salt是大肠杆菌（菌株K12，MG 1655）中产生的代谢产物[2]。
在体外，NADPH tetrasodium salt（100µM）处理HUVEC和PIEC细胞30分钟，显著增加了两种细胞的O2−产量[3]。NADPH tetrasodium salt（100µM）加入到再浓缩肺动脉匀浆中，显著升高了可溶性鸟苷酸环化酶（sGC）的活性，使基础活性增加了2.2±1.0倍[4]。NADPH tetrasodium salt（0-300µM）处理诱导啮齿动物β细胞，剂量依赖性地诱导了Ca2+依赖性胞吐作用[5]。
在体内，NADPH tetrasodium salt（16mg/kg）静脉注射处理雄性SD大鼠，给药后60 min内+dp/dt显著升高，-dp/dt显著降低，增强了大鼠体内心脏功能[2]。NADPH tetrasodium salt（2.5mg/kg）静脉注射治疗大鼠和小鼠中风模型，显著减少了脑梗死体积，改善神经功能，提高存活率[6]。
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Cell experiment [1]: |
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Cell lines |
HUVEC cells、PIEC cells |
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Preparation method |
Cells were resuspended in DMEM without phenol red and incubated in 96-well flat-bottomed cell culture plates (2 × 105 cells/well) for 10 min at 37°C in a humidified CO2 incubator. Cytochrome c (final concentration 250–2000 μmol/l) and NADPH tetrasodium salt (100μmol/l) were added to the cells and incubated for 30 min. |
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Reaction Conditions |
100μmol/L; 30 min |
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Applications |
Addition of NADPH tetrasodium salt significantly increased O2− production in both cell types. |
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Animal experiment [2]: |
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Animal models |
Male SD rats |
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Preparation method |
The rats were randomly allocated to the control and experimental groups. The maximum up rate of left ventricular pressure(+dp/dtmax), and the maximum down rate of left ventricular pressure(−dp/dtmax) were recorded at baseline and after the intravenous administration of NADPH tetrasodium salt (16mg/kg). Subsequently, the rats were euthanized under anesthesia. |
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Dosage form |
16mg/kg；i.v. |
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Applications |
The results showed that +dp/dt was significantly increased while the −dp/dt was decreased within 60 min after NADPH tetrasodium salt administration, suggesting that NADPH tetrasodium salt can enhance the cardiac function of rats in vivo. |
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References: [1] Li,J. Differential NADPH- versus NADH-dependent superoxide production by phagocyte-type endothelial cell NADPH oxidase[J].Cardiovascular Research, 2001, 52(3):477. [2] Qian K, Tang J, Ling Y J, et al. Exogenous NADPH exerts a positive inotropic effect and enhances energy metabolism via SIRT3 in pathological cardiac hypertrophy and heart failure[J]. EBioMedicine, 2023, 98. |
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| Cas No. | 2646-71-1 | SDF | |
| 别名 | 还原型辅酶Ⅱ四钠 | ||
| Canonical SMILES | [O-]P(OP([O-])(OC[C@@H]1[C@@H](O)[C@@H](OP([O-])([O-])=O)[C@H](N2C3=NC=NC(N)=C3N=C2)O1)=O)(OC[C@@H]4[C@@H](O)[C@@H](O)[C@H](N5C=C(C(N)=O)CC=C5)O4)=O.[Na+].[Na+].[Na+].[Na+] | ||
| 分子式 | C21H26N7Na4O17P3 | 分子量 | 833.35 |
| 溶解度 | Water : ≥ 35 mg/mL (42.00 mM); < 1 mg/mL in DMSO (ultrasonic;warming;heat to 60°C) (insoluble or slightly soluble) | 储存条件 | Store at -20°C, stored under nitrogen |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 1.2 mL | 5.9999 mL | 11.9998 mL |
| 5 mM | 0.24 mL | 1.2 mL | 2.4 mL |
| 10 mM | 0.12 mL | 0.6 mL | 1.2 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
