Naphthoresorcinol
(Synonyms: 1,3-二羟基萘; 1,3-Dihydroxynaphthalene) 目录号 : GC61712Naphthoresorcinol(1,3-Dihydroxynaphthalene)是一种荧光染料(Λex=330nm,Λem=380nm),可以与NPPD(一种示踪剂)和浓HCl反应并显示出红色。Naphthoresorcinol也可以用作背景电解质(BGE)来测定碳水化合物。
Cas No.:132-86-5
Sample solution is provided at 25 µL, 10mM.
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Naphthoresorcinol (1,3-Dihydroxynaphthalene) is a fluorescent dye (Λex=330 nm, Λem=380 nm) that can react with the NPPD (a tracer) and concentrated HCl and develop a red color. Naphthoresorcinol could be used as a background electrolyte (BGE) to determine the carbohydrates[1][2][3].
[1]. Vyas S, et, al. Fluorescence and light scattering studies on the interaction of 1,3-dihydroxynaphthalene with ionic and non-ionic surfactants. Polymer Photochemistry. 1984; 4(4):245-253. [2]. Suzuki S, et, al. Development of a field kit for use by non-scientists for chemical tracking using 5-(4-nitrophenyl)-2,4-pentadien-1-al. Forensic Sci Int. 2013 May 10;228(1-3):e25-7. [3]. Lee YH, et, al. Determination of carbohydrates by high-performance capillary electrophoresis with indirect absorbance detection. J Chromatogr B Biomed Appl. 1996 May 31;681(1):87-97.
Cas No. | 132-86-5 | SDF | |
别名 | 1,3-二羟基萘; 1,3-Dihydroxynaphthalene | ||
Canonical SMILES | OC1=C2C=CC=CC2=CC(O)=C1 | ||
分子式 | C10H8O2 | 分子量 | 160.17 |
溶解度 | DMSO : 100 mg/mL (624.34 mM; Need ultrasonic) | 储存条件 | Store at -20°C |
General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效。 储备液的保存方式和期限:-80°C 储存时,请在 6 个月内使用,-20°C 储存时,请在 1 个月内使用。 为了提高溶解度,请将管子加热至37℃,然后在超声波浴中震荡一段时间。 |
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Shipping Condition | 评估样品解决方案:配备蓝冰进行发货。所有其他可用尺寸:配备RT,或根据请求配备蓝冰。 |
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1 mg | 5 mg | 10 mg | |
1 mM | 6.2434 mL | 31.2168 mL | 62.4337 mL |
5 mM | 1.2487 mL | 6.2434 mL | 12.4867 mL |
10 mM | 0.6243 mL | 3.1217 mL | 6.2434 mL |
第一步:请输入基本实验信息(考虑到实验过程中的损耗,建议多配一只动物的药量) | ||||||||||
给药剂量 | mg/kg | 动物平均体重 | g | 每只动物给药体积 | ul | 动物数量 | 只 | |||
第二步:请输入动物体内配方组成(配方适用于不溶于水的药物;不同批次药物配方比例不同,请联系GLPBIO为您提供正确的澄清溶液配方) | ||||||||||
% DMSO % % Tween 80 % saline | ||||||||||
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工作液浓度: mg/ml;
DMSO母液配制方法: mg 药物溶于 μL DMSO溶液(母液浓度 mg/mL,
体内配方配制方法:取 μL DMSO母液,加入 μL PEG300,混匀澄清后加入μL Tween 80,混匀澄清后加入 μL saline,混匀澄清。
1. 首先保证母液是澄清的;
2.
一定要按照顺序依次将溶剂加入,进行下一步操作之前必须保证上一步操作得到的是澄清的溶液,可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Mutagenicity of products formed by ozonation of Naphthoresorcinol in aqueous solutions
Mutat Res 1987 Nov;189(3):217-22.PMID:2959862DOI:10.1016/0165-1218(87)90055-3.
The mutagenicity of products formed by ozonation of Naphthoresorcinol in aqueous solution was assayed with Salmonella typhimurium strains TA97, TA98, TA100, TA102 and TA104 in the presence and absence of S9 mix from phenobarbital- and 5,6-benzoflavone-induced rat liver. Ozonated Naphthoresorcinol was mutagenic in TA97, TA98, TA100 and TA104 without S9 mix. By the addition of S9 mix, the mutagenic activity of ozonated Naphthoresorcinol was markedly suppressed in TA98 and TA100, but became positive in TA102. High-performance liquid chromatography (HPLC) after derivatization to 2,4-dinitrophenylhydrazones demonstrated the formation of glyoxal as an ozonation product of Naphthoresorcinol. Ion chromatographic technique also demonstrated the formation of o-phthalic acid, muconic acid, maleic acid, mesoxalic acid, glyoxylic acid and oxalic acid as ozonation products. The mutagenicity assays of these identified products with five Salmonella showed that glyoxal and glyoxylic acid were directly mutagenic; the former in TA100, TA102 and TA104, the latter in TA97, TA100 and TA104. In the presence of S9 mix, glyoxylic acid gave a positive response of mutagenicity for TA102. The experimental evidence supported that glyoxal and glyoxylic acid may contribute to the mutagenicity of ozonated Naphthoresorcinol.
Mutagenicity on chlorination of products formed by ozonation of Naphthoresorcinol in water
Mutat Res 1989 Jul;226(3):151-5.PMID:2664498DOI:10.1016/0165-7992(89)90012-2.
The mutagenicity of products formed by chlorination after ozonation of Naphthoresorcinol in aqueous solution was assayed with Salmonella typhimurium strains TA98 and TA100 in the presence and absence of S9 mix from phenobarbital- and 5,6-benzoflavone-induced rat liver. Ozonated and subsequently chlorinated Naphthoresorcinol was directly mutagenic, as was ozonated Naphthoresorcinol, in both strains tested. The mutagenic activity at chlorination with 8 equivalents of chlorine per mole of Naphthoresorcinol after ozonation was markedly higher than that at only ozonation. Of the identified ozonation products of Naphthoresorcinol, muconic acid, after chlorination with 2 or 4 equivalents of chlorine per mole of the compound, induced direct mutagenicity against TA98 and TA100. The chlorination of glyoxal with 0.5 and 1 chlorine equivalents per mole of the compound was shown to produce direct mutagenicity toward TA98. The identification of the chlorination products of these compounds is also discussed.
Multifunctional lanthanide metal-organic framework based ratiometric fluorescence visual detection platform for alkaline phosphatase activity
Mikrochim Acta 2021 Jun 24;188(7):236.PMID:34165637DOI:10.1007/s00604-021-04880-4.
A turn-on/off ratiometric fluorescence detection platform based on multifunctional lanthanide metal-organic framework (Ln-MOF) and an enzymatic cascade reaction is proposed for alkaline phosphatase (ALP) activity assay. L-phosphotyrosine is hydrolyzed to levodopa (L-dopa) by two steps of enzymatic reaction. L-dopa further reacts with Naphthoresorcinol to produce carboxyazamonardine with strong emission at 490 nm. In this process, multifunctional Ln-MOF (Cu@Eu-BTC, BTC is the 1,3,5-benzenetricarboxylic acid) acts not only as a nanozyme to catalyze the fluorogenic reaction between L-dopa and Naphthoresorcinol but also as a fluorescence internal standard. The emission of Cu@Eu-BTC at 620 nm is quenched by phosphate anions, and the dual-response ratiometric fluorescence (F490/F620) can be achieved. A good linear relationship was obtained between Δ(F490/F620) and ALP activity in the range 0.3-24 U L-1 with the detection limit of 0.02 U L-1. In addition, a portable assay tube was designed for visual and point-of-care testing of ALP activity by color variation (ratiometric chromaticity). Both the ratiometric fluorescence detection and the visual detection methods were successfully applied to monitor ALP activity in human serum samples with recovery between 95.5%-109.0% and 94.0%-110.1%, and relative standard deviation less than 8.1% and 9.5%, respectively. As far as we know, this is the first report of ALP activity assay assisted by multifunctional Ln-MOF.Graphical abstract.
Development of a field kit for use by non-scientists for chemical tracking using 5-(4-nitrophenyl)-2,4-pentadien-1-al
Forensic Sci Int 2013 May 10;228(1-3):e25-7.PMID:23522523DOI:10.1016/j.forsciint.2013.02.043.
5-(4-Nitrophenyl)-2,4-pentadien-1-al (NPPD) can be used for chemical tracking in crime scene investigations. A color test kit for NPPD was developed for use by non-scientists, such as police officers, in the field. However, this kit had problems, including contact with concentrated HCl, and instability of the reagent (Naphthoresorcinol methanol solution) used in the first step of color development. To overcome these problems, in the present study, a field kit was developed with the concentrated HCl sealed in a vial so it did not contact the operator. A glass tube with two compartments was used to separate the Naphthoresorcinol and methanol before use. When the color test was conducted, a cotton swab was inserted into the tube. Before insertion, the cotton was used to collect a sample from a suspect that had been in contact with a surface sprayed with a 1% NPPD methanol solution. Insertion of the cotton swab broke the thin glass that separated the methanol and Naphthoresorcinol, and any NPPD on the swab reacted with the Naphthoresorcinol methanol solution. The cotton swab was then pushed further to break the glass separating the concentrated HCl. A red color then developed if NPPD was present on the cotton swab. For testing the kit, NPPD was sprayed in an area where a crime was expected to occur. This kit will be useful for detecting a contact with or near a crime scene, because samples do not require analysis in a forensic science laboratory. Instead, the results can be confirmed at the scene of crime.
Mutagenicity of dihydroxybenzenes and dihydroxynaphthalenes for Ames Salmonella tester strains
Mutat Res 1996 Dec 20;371(3-4):293-9.PMID:9008731DOI:10.1016/s0165-1218(96)90118-4.
The mutagenicity of 3 dihydroxybenzene (DHB) and 9 dihydroxynaphthalene (DHN) isomers was examined by using 5 different Ames Salmonella mutagenicity tester strains in the presence and absence of phenobarbital and 5,6-benzoflavone-treated rat liver S9-mix. Of the 3 DHB isomers, 1,4-DHB (hydroquinone) was mutagenic, and of the 9 DHN isomers, 1,3-DHN (Naphthoresorcinol), 1,4-DHN (hydronaphthoquinone), 1,6-DHN and 1,7-DHN were mutagenic. Mutagenicity of all the compounds tested was observed in the absence of S9-mix, while 1,4-DHN and 1,6-DHN were also mutagenic in the presence of S9-mix. The mutagenicity of 1,4-DHB and 1,4-DHN for TA104, which is a strain sensitive to oxidative mutagens, was almost completely or partially inhibited by superoxide dismutase (SOD) and/or catalase, indicating the involvement of activated oxygen species in mutagenesis. Furthermore, from the finding that the 4 DHNs were mutagenic for TA2637, the strain sensitive to frameshift mutagens, it is possible that the mutagenicity of DHNs for S. typhimurium was also attributable to DNA adducts that form with quinones and/or semiquinones through oxidation of DHNs. The mutagenicity of 1,3-DHN, which showed the largest number of revertants in strains TA100, TA98, TA2637 and TA104, was greatly decreased, when their pKM101 plasmid-deficient strains, TA1535, TA1538, TA1537 and TA2659 were used. This observation suggests that an SOS repair system was involved in the mutagenesis of 1,3-DHN for S. typhimurium.